Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 571,620 G→A 10.5% intergenic (+43/+48) ssb → / ← PP_0486 single‑stranded DNA‑binding protein/GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947571,6200GA10.5% 152.0 / 7.8 57intergenic (+43/+48)ssb/PP_0486single‑stranded DNA‑binding protein/GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (29/22);  new base A (2/4);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

CCCAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGATGCGCA  >  NC_002947/571477‑571768
                                                                                                                                               |                                                                                                                                                    
cccAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGccc                                                                                                                                                >  2:1086568/1‑150 (MQ=255)
 ccAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCt                                                                                                                                               >  1:794040/1‑150 (MQ=255)
 ccAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCt                                                                                                                                               >  2:632425/1‑150 (MQ=255)
  cAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTg                                                                                                                                              <  2:1151298/150‑1 (MQ=255)
   aGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAACTGCCCTgg                                                                                                                                             <  2:936063/150‑1 (MQ=255)
       cccGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGttt                                                                                                                                         <  2:837464/150‑1 (MQ=255)
            aaCAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTa                                                                                                                                    >  1:381966/1‑150 (MQ=255)
              cagtgcccTCAGCGGCCAGCGCCGCAACAGCCTGCGGCGCAGCCGGCGGCTGACTTCGACAGCTGCGATGACGATATTCCGTTCTGATCAGAGGGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATg                                                                                                                                  <  2:27888/145‑1 (MQ=255)
                ggCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATggg                                                                                                                                <  2:783973/150‑1 (MQ=255)
                   cccTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgcag                                                                                                                             >  2:835644/1‑150 (MQ=255)
                   cccTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgcag                                                                                                                             <  1:763805/150‑1 (MQ=255)
                     cTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgcagca                                                                                                                           >  2:605403/1‑150 (MQ=255)
                             ccagcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTAt                                                                                                                   >  2:684930/1‑150 (MQ=255)
                                    cgcAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCAt                                                                                                            >  2:639789/1‑150 (MQ=255)
                                           gCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCATGGCAGTTTCCCAGGGGCGTTTTATGGGCTGCACCTCGTATCAGGCATCCCTTTc                                                                                                     >  2:253512/1‑150 (MQ=255)
                                              tGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCAt                                                                                                            >  1:396788/1‑140 (MQ=255)
                                              tGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCAt                                                                                                            <  2:396788/140‑1 (MQ=255)
                                               gcgcCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTa                                                                                                 <  1:684930/150‑1 (MQ=255)
                                                      aGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGaacaac                                                                                          >  2:907229/1‑150 (MQ=255)
                                                                tGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCg                                                                                <  1:639789/150‑1 (MQ=255)
                                                                                 aTGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCtt                                                               <  1:656127/150‑1 (MQ=255)
                                                                                  tGACCATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGAACAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTg                                                              <  2:375561/150‑1 (MQ=255)
                                                                                     cGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCa                                                           >  2:1119017/1‑150 (MQ=255)
                                                                                      gATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATg                                                                                                                                  <  1:769983/78‑1 (MQ=255)
                                                                                      gATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATg                                                                                                                                  >  2:769983/1‑78 (MQ=255)
                                                                                        tatTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCt                                                        >  2:1145108/1‑150 (MQ=255)
                                                                                         atTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCtt                                                       <  1:702237/150‑1 (MQ=255)
                                                                                          ttCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTg                                                      >  2:194830/1‑150 (MQ=255)
                                                                                               ttCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAg                                                 <  1:605403/150‑1 (MQ=255)
                                                                                                tCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGACCAACCGGGGCTCCGGCGAATCCAACAGGCTTGCCAGCTTGGTCAgg                                                <  2:381966/150‑1 (MQ=255)
                                                                                                    aTCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCaa                                            >  2:1071984/1‑150 (MQ=255)
                                                                                                           cGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAg                                     <  1:1071984/150‑1 (MQ=255)
                                                                                                             gTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGtt                                   <  2:431750/150‑1 (MQ=255)
                                                                                                              tAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTc                                  <  1:835644/150‑1 (MQ=255)
                                                                                                                gggCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACcgc                                                                                                                       >  2:1140662/1‑63 (MQ=255)
                                                                                                                gggCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACcgc                                                                                                                       <  1:1140662/63‑1 (MQ=255)
                                                                                                                  gCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTgg                              >  1:18954/1‑150 (MQ=255)
                                                                                                                         gCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATTGTTATCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTgg                       >  2:517145/1‑150 (MQ=255)
                                                                                                                          cAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTggg                      >  1:1037730/1‑150 (MQ=255)
                                                                                                                          cAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTggg                      >  1:1031519/1‑150 (MQ=255)
                                                                                                                          cAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTggg                      >  1:660565/1‑150 (MQ=255)
                                                                                                                           aaGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGt                     <  1:632425/150‑1 (MQ=255)
                                                                                                                             gACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGc                                                             >  2:949666/1‑108 (MQ=255)
                                                                                                                             gACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGc                                                             <  1:949666/108‑1 (MQ=255)
                                                                                                                                cAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCAt                >  2:1018442/1‑150 (MQ=255)
                                                                                                                                 aGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTgg                              <  1:1179091/135‑1 (MQ=255)
                                                                                                                                 aGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTgg                              >  2:1179091/1‑135 (MQ=255)
                                                                                                                                 aGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATc               >  2:625661/1‑150 (MQ=255)
                                                                                                                                     aCCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAggg           >  2:537512/1‑150 (MQ=255)
                                                                                                                                     aCCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAggg           >  1:921822/1‑150 (MQ=255)
                                                                                                                                     aCCCAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTcc                                                                                 <  2:586698/80‑1 (MQ=255)
                                                                                                                                     aCCCAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTcc                                                                                 >  1:586698/1‑80 (MQ=39)
                                                                                                                                      cccAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGc          <  1:205543/150‑1 (MQ=255)
                                                                                                                                      cccAGGGCAACTGCCCTGGGTTTCTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTAGCGGCTGGGTGCCATCAGGGc          >  2:473847/1‑150 (MQ=255)
                                                                                                                                        cAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGa        >  1:313437/1‑150 (MQ=255)
                                                                                                                                          gggCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTg                               <  1:1157994/125‑1 (MQ=255)
                                                                                                                                              aGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGATGCGca  <  1:165311/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CCCAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGATGCGCA  >  NC_002947/571477‑571768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑