Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 70,759 G→C 7.6% G382G (GGC→GGG)  glyS ← glycine‑tRNA ligase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294770,7590GC 7.6% 150.7 / 2.7 49G382G (GGC→GGG) glySglycine‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (24/21);  new base C (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TGCTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTC  >  NC_002947/70622‑70906
                                                                                                                                          |                                                                                                                                                   
tGCTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGCGACGCCCAGCGCGCTg                                                                                                                                          >  1:453612/1‑150 (MQ=255)
  cTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGc                                                                                                                                       >  2:829779/1‑150 (MQ=255)
  cTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGc                                                                                                                                       >  2:829976/1‑150 (MQ=255)
  cTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGc                                                                                                                                       >  1:408696/1‑150 (MQ=255)
      ttCAGGTCCAGGACTACGTCTTCCGGTTCACCGTCGGTGGGCGTGTAGTAGTAAACGGCAACGCCCTGCGTCTCAGGGAATTCGCGGACCATCGCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGGCAGCGCGCTGGtcgtcg                                                                                                                                   >  2:961888/1‑150 (MQ=255)
       tCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGCGACGCCCAGCGCGCTGGCCGtgc                                                                                                                                   <  2:453612/150‑3 (MQ=255)
        cagtgccaggtcTACGTCTCCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATGCGCCGACCATCTCGGTGGCCANGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTcgcc                                                                                                                                 <  1:623187/150‑1 (MQ=255)
          gggccagggcTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTcgccgc                                                                                                                               >  2:1106757/1‑150 (MQ=255)
            gccagggcTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGc                                                                                                                                       <  2:540187/140‑1 (MQ=255)
            gccagggcTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGc                                                                                                                                       >  1:540187/1‑140 (MQ=255)
                  gcTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGc                                                                                                                       >  2:912754/1‑150 (MQ=255)
                                   aCCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGACCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGACCAATGAAgccggc                                                                                                      >  2:431498/1‑150 (MQ=255)
                                                gcgTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGCGACGCCCAGCGCGCTGGCCGt                                                                                                                                     <  2:245386/107‑1 (MQ=255)
                                                gcgTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGCGACGCCCAGCGCGCTGGCCGt                                                                                                                                     >  1:245386/1‑107 (MQ=255)
                                                gcgTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAaca                                                                                         >  2:211370/1‑150 (MQ=255)
                                                  gtagtagtaACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGGCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACg                                                                                       >  2:362104/1‑150 (MQ=255)
                                                          aaCCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCt                                                                               >  1:902945/1‑150 (MQ=255)
                                                           aCCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCt                                                                                                                                           <  1:601561/89‑1 (MQ=255)
                                                           aCCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCt                                                                                                                                           >  2:601561/1‑89 (MQ=255)
                                                              ggCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCCCTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAACAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTc                                                                           <  1:1149976/150‑1 (MQ=255)
                                                              ggCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTc                                                                           <  1:829976/150‑1 (MQ=255)
                                                              ggCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTc                                                                           <  1:912754/150‑1 (MQ=255)
                                                              ggCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTc                                                                           <  1:829779/150‑1 (MQ=255)
                                                                     cccTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTAAACg                                                                    <  2:1121672/150‑1 (MQ=255)
                                                                      ccTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACgg                                                                   >  1:820017/1‑150 (MQ=255)
                                                                        tGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGa                                                                                                                    <  2:115869/99‑1 (MQ=255)
                                                                        tGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGa                                                                                                                    >  1:115869/1‑99 (MQ=255)
                                                                            tttCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGa                                                             <  2:902945/150‑1 (MQ=255)
                                                                             ttCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCg                                                                                   >  1:1067294/1‑127 (MQ=255)
                                                                             ttCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCg                                                                                   <  2:1067294/127‑1 (MQ=255)
                                                                                aGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCg                                                                                   <  1:959379/124‑1 (MQ=255)
                                                                                aGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCg                                                                                   >  2:959379/1‑124 (MQ=255)
                                                                                    aaTTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGcc                                                     >  2:969578/1‑150 (MQ=255)
                                                                                      ttCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTg                                                   <  2:408696/150‑1 (MQ=255)
                                                                                         gCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGaa                                                <  2:486528/150‑1 (MQ=255)
                                                                                              ccATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAAcacca                                           >  2:174021/1‑150 (MQ=255)
                                                                                                     ggTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGACGGCGGCCCGCAAGGCCAAGCGTTATTCCTGGTCGGGGACGGTGCCGTGCTGAGCCTGGAACACCACGTTCtt                                    >  1:871799/1‑150 (MQ=255)
                                                                                                      gTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTg                                   <  1:211370/150‑1 (MQ=255)
                                                                                                         gCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCGCGTTCTTGAgg                                <  1:969578/150‑1 (MQ=255)
                                                                                                             ggTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCt                            >  2:725232/1‑150 (MQ=255)
                                                                                                              gTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTc                           >  2:448083/1‑150 (MQ=255)
                                                                                                                     ttCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAg                    >  1:365243/1‑150 (MQ=255)
                                                                                                                        gACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGaa                     <  2:1205247/146‑1 (MQ=255)
                                                                                                                        gACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGaa                     >  1:1205247/1‑146 (MQ=255)
                                                                                                                        caCAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCAAATTTTCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGtt                 <  1:316905/149‑1 (MQ=255)
                                                                                                                         aCAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGttt                >  1:786690/1‑150 (MQ=255)
                                                                                                                              agGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGt           <  1:638536/150‑1 (MQ=255)
                                                                                                                                gCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTgg         <  1:1106757/150‑1 (MQ=255)
                                                                                                                                        cGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGcttc  <  1:362104/150‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
TGCTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTC  >  NC_002947/70622‑70906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑