Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	584,247	A→C	7.2%	intergenic (+85/+22)	PP_t75 → / ← ansA	tRNA‑Sec/type 1 L‑asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	584,247	0	A	C	7.2%	178.5 / 3.6	64	intergenic (+85/+22)	PP_t75/ansA	tRNA‑Sec/type 1 L‑asparaginase
Reads supporting (aligned to +/- strand):  ref base A (25/34);  new base C (3/2);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CTTCAAACCCGGTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGTCATGCCACCGCCACT  >  NC_002947/584099‑584391                                                                                                                                                     |                                                                                                                                                 cTTCAAACCCGGTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGaa                                                                                                                                                 >  2:1111696/1‑150 (MQ=255)     aaaCCCGGTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAgg                                                                                                                                             >  1:1017856/1‑150 (MQ=255)     aaaCCCGGTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAgg                                                                                                                                             <  1:1111696/150‑1 (MQ=255)      aaCCCGGTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGc                                                                                                                                            >  1:1044724/1‑150 (MQ=255)        cccGGTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGccc                                                                                                                                          <  1:1123185/150‑1 (MQ=255)               ggggACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAggg                                                                                                                                   <  1:1176965/150‑1 (MQ=255)                     ggCAGCCGTTCCCGGGCAGGTTCGACGCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTCCCCGCGAAGAGGCCCTCAAGGGCGCTGa                                                                                                                             <  2:569205/150‑1 (MQ=255)                         gcggTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCaa                                                                                                                         >  1:1205479/4‑150 (MQ=255)                         gCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCCCCCTTTAGGGGCTCTTCGCGCGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCaa                                                                                                                         <  1:648281/150‑1 (MQ=255)                         gCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCaa                                                                                                                         <  2:1193253/150‑1 (MQ=255)                                 cGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTc                                                                                                                 <  1:470075/150‑1 (MQ=255)                                     cAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCca                                                                                                             <  1:892911/150‑1 (MQ=255)                                       ggTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGCGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCcaca                                                                                                           <  1:333868/150‑1 (MQ=255)                                         ttCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGcc                                                                                                                      <  2:1178700/137‑1 (MQ=255)                                         ttCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGGGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGcc                                                                                                                      >  1:1178700/1‑137 (MQ=255)                                           cGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACaaa                                                                                                       <  2:1044724/150‑1 (MQ=255)                                             ccTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCGGTTGGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATc                                                                                                     <  2:790597/149‑1 (MQ=255)                                              cTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATcc                                                                                                    >  2:766569/1‑150 (MQ=255)                                               tCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCa                                                                                                   >  1:1018383/1‑150 (MQ=255)                                               tCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCa                                                                                                   <  2:829919/150‑1 (MQ=255)                                               tCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCa                                                                                                   <  2:981031/150‑1 (MQ=255)                                                         ttCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCa                                                                                         <  2:606017/150‑1 (MQ=255)                                                         ttCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCa                                                                                         >  2:658164/1‑150 (MQ=255)                                                              ccATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGt                                                                                    >  1:404097/1‑150 (MQ=255)                                                               cATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGt                                                                                    <  2:1029306/149‑1 (MQ=255)                                                               cATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGt                                                                                    >  1:1029306/1‑149 (MQ=255)                                                                        aCCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTc                                                                                                                                        >  1:1207503/1‑88 (MQ=255)                                                                        aCCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTc                                                                                                                                        <  2:1207503/88‑1 (MQ=255)                                                                        aCCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTc                                                                          <  1:658164/150‑1 (MQ=255)                                                                        aCCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGATTACCCGCGCAGAAGCCCACAAGGGCGCTGCTAAAGCCCGCTCGCCACACACCTCCAGGACAAACCAGCGGACTGATGCGTc                                                                          >  2:320519/1‑150 (MQ=255)                                                                           cTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGc                                                                                                                       >  2:982292/1‑102 (MQ=255)                                                                           cTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGc                                                                                                                       <  1:982292/102‑1 (MQ=255)                                                                            tttAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCa                                                                      >  2:602171/1‑150 (MQ=255)                                                                                   gCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAg                                                                                        >  2:79654/1‑125 (MQ=255)                                                                                   gCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAg                                                                                        <  1:79654/125‑1 (MQ=255)                                                                                          gcgGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGcc                                                        <  1:602171/150‑1 (MQ=255)                                                                                              gTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCa                                                                                         >  2:332083/1‑113 (MQ=255)                                                                                              gTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCa                                                                                         <  1:332083/113‑1 (MQ=255)                                                                                                gAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAg                                                  >  1:1126267/1‑150 (MQ=255)                                                                                                  aCCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAg                                                     <  2:949006/145‑1 (MQ=255)                                                                                                  aCCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAg                                                     >  1:949006/1‑145 (MQ=255)                                                                                                   cccGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGTGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCa                                                      <  2:645854/143‑1 (MQ=255)                                                                                                   cccGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGTGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCa                                                      >  1:645854/1‑143 (MQ=255)                                                                                                            acacAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCtt                                      <  2:458833/150‑1 (MQ=255)                                                                                                             cacaGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTg                                     >  1:465124/1‑150 (MQ=255)                                                                                                                aGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGccc                                  >  1:473903/1‑150 (MQ=255)                                                                                                                  gAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAg                                >  2:457034/1‑150 (MQ=255)                                                                                                                   aaaCGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAgg                               <  2:404097/150‑1 (MQ=255)                                                                                                                   aaaCGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAgg                               <  1:1077650/150‑1 (MQ=255)                                                                                                                    aaCGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAGGCCAGGACAAACCAGCGGTCTGCTGCGTAAGAATCAAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAggg                              >  1:79438/1‑150 (MQ=255)                                                                                                                       gCAATCGTCGTAGGCTCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGAGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGgctg                           <  1:422664/150‑1 (MQ=255)                                                                                                                       gCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGgctg                           >  2:803880/1‑150 (MQ=255)                                                                                                                           tcgtcgTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTc                       <  1:766569/150‑1 (MQ=255)                                                                                                                              tcgtAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCAcc                    >  2:255232/1‑149 (MQ=255)                                                                                                                                 tcttcgcgGGTAAACCCGCGCAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAAcc                                                               >  1:37744/5‑104 (MQ=255)                                                                                                                                 tcttcgcgGGTAAACCCGCGCAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAAcc                                                               <  2:37668/100‑1 (MQ=255)                                                                                                                                 tcttcgcgGGTAAACCCGCGCAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAAcc                                                               <  2:37744/100‑1 (MQ=255)                                                                                                                                 tcttcgcgGGTAAACCCGCGCAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAAcc                                                               >  1:37668/5‑104 (MQ=255)                                                                                                                                    tcgcgGGTAAACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGTCAt              >  2:920448/2‑150 (MQ=255)                                                                                                                                    gcgcGTGTTGCCCCGCGAAGAAGCTCCACATAGCTCTGATCAAGCCCCCTCGCCACACAAATCCCGGACAAACCAGCGATCTGCCGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCACCATGCCCACGGCTGCTGCACGGATCAt              <  2:79438/150‑1 (MQ=255)                                                                                                                                      gcgGGTAAACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAg                                                     <  2:873254/109‑1 (MQ=255)                                                                                                                                              aCCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGTCATGCCACCGCCa    <  2:788818/150‑1 (MQ=255)                                                                                                                                              aCCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGTCATGCCACCGCCa    <  1:457034/150‑1 (MQ=255)                                                                                                                                                ccGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGTCATGCCACCGCCACt  >  2:234145/1‑150 (MQ=255)                                                                                                                                                ccGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGTCATGCCACCGCCACt  <  2:465124/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CTTCAAACCCGGTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGTCATGCCACCGCCACT  >  NC_002947/584099‑584391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑