Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 587,495 A→G 8.5% N182D (AAC→GAC)  PP_0497 → Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947587,4950AG 8.5% 132.5 / 2.6 47N182D (AAC→GAC) PP_0497Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (20/23);  new base G (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

GCCGTTTTCTGTTCGAGCGCCAGGAACGGCAGGAGCGCTCCAGCGCCACCGATCAGCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAGCGGCGACCGGCGCAACCCAGCCTGTACCCGGGTGGTGTGGGAATACCTGGGCGAGGAGATCAA  >  NC_002947/587352‑587640
                                                                                                                                               |                                                                                                                                                 
gCCGTTTTCTGTTCGAGCGCCAGGAACGGCAGGAGCGCTCCAGCGCCACCGATCAGCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGaaa                                                                                                                                             >  2:989670/1‑150 (MQ=255)
     tttCTGTTCGAGCGCCAGGAACGGCAGGAGCGCTCCAGCGCCACCGATCAGCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGc                                                                                                                                        <  2:703082/150‑1 (MQ=255)
     tttCTGTTCGAGCGCCAGGAACGGCAGGAGCGCTCCAGCGCCACCGATCAGCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGc                                                                                                                                        <  2:758821/150‑1 (MQ=255)
           ttCGAGCGCCAGGAACGGCAGGAGCGCTCCACCGCAAAGGCACGTTAATGATTGTGACGTTGCCGCTAATTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGAcc                                                                                                                                  >  1:511069/1‑150 (MQ=255)
                        aaCGGCAGGAGCGCTCCAGCGCCACCGATCAGCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCa                                                                                                                     <  2:1197681/150‑1 (MQ=255)
                                    gcTCCAGCGCCACCGATCAGCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCGACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGcc                                                                                                         >  1:611631/1‑150 (MQ=255)
                                      tCCAGCGCCACCGATCAGCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGc                                                                                                       >  1:446810/1‑150 (MQ=255)
                                          gcgcCACCGATCAGCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTc                                                                                                                >  1:583191/1‑137 (MQ=255)
                                          gcgcCACCGATCAGCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTc                                                                                                                <  2:583191/137‑1 (MQ=255)
                                               aCCGATCAGCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCgg                                                                                              >  1:212484/1‑150 (MQ=255)
                                                 cGATCAGCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCt                                                                                            <  2:356033/150‑1 (MQ=255)
                                                      aGCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATg                                                                                       >  1:446695/1‑150 (MQ=255)
                                                       gCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCGACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATgg                                                                                      <  2:611631/150‑1 (MQ=255)
                                                          tGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGCTAGGATGTTTCCGATGGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCtt                                                                                   <  2:739151/150‑1 (MQ=255)
                                                          tGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAg                                                                                                           <  2:805575/126‑1 (MQ=255)
                                                          tGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAg                                                                                                           >  1:805575/1‑126 (MQ=255)
                                                          tGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCtt                                                                                   >  2:1182936/1‑150 (MQ=255)
                                                            ccGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCAAGACCATCCTCATGGCCAGCCGCAAAAGCCGCAGTGTCCGGCTGAATGGCTTGa                                                                                 >  2:800875/1‑150 (MQ=255)
                                                             cGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATcc                                                                                                                              >  2:855952/1‑104 (MQ=255)
                                                             cGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATcc                                                                                                                              <  1:855952/104‑1 (MQ=255)
                                                              gAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAg                                                                               >  1:1093948/1‑150 (MQ=255)
                                                                      cTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGt                                                                       >  1:608910/1‑150 (MQ=255)
                                                                        gCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTAt                                                                     <  2:446810/150‑1 (MQ=255)
                                                                              aCCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAgcg                                                               <  1:1170666/150‑1 (MQ=255)
                                                                                       cggcgTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGCATGGCTTGAAGGCCGGCGCATCCAGCGGCGAcagac                                                      <  2:923877/150‑5 (MQ=255)
                                                                                        ggcgTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAGCGGCGACCGgcg                                                     >  1:1142681/1‑150 (MQ=255)
                                                                                             tCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAGCGGCGACCGGCGCAAcc                                                <  2:1181377/150‑1 (MQ=255)
                                                                                               cAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCt                                                                                    >  2:651859/1‑112 (MQ=255)
                                                                                               cAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCt                                                                                    <  1:651859/112‑1 (MQ=255)
                                                                                                      aGGACCGGATGTTCCTCATCGACTACGAGTCGCCGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAGCGGCGACCGGCGCAACCCAGCCTGTa                                       >  1:886746/1‑150 (MQ=255)
                                                                                                           cGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCGACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCtt                                                                                   >  1:824173/1‑101 (MQ=255)
                                                                                                           cGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCGACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCtt                                                                                   <  2:824173/101‑1 (MQ=255)
                                                                                                           cGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAGCGGCGACCGGCGCAACCCAGCCTGTACCCgg                                  <  1:118846/150‑1 (MQ=255)
                                                                                                            ggATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAGCGGCGACCGGCGCAACCCCACCTGTACCCggg                                 >  1:185396/1‑150 (MQ=255)
                                                                                                                        tCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCa                                                                  >  2:597837/1‑105 (MQ=255)
                                                                                                                        tCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCa                                                                  <  1:597837/105‑1 (MQ=255)
                                                                                                                          gACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAGCGGCGACCGGCGCAACCCAGCCTGTACCCGGGTGGTGTGGGAATAc                   <  2:212484/150‑1 (MQ=255)
                                                                                                                               cGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAGCGGCGACCGGCGCAACCCAGCCTGTACCCGGGTGGTGTGGGAATACCTggg              <  1:1182936/150‑1 (MQ=255)
                                                                                                                                gAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGTCGTATCCAGCGGCGACCGGCGCAACCCAGCCTGTACCCGGGTGGTGTGGGAATACCTGGGc             <  2:511069/150‑1 (MQ=255)
                                                                                                                                gAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAGCGGCGACCGGCGCAACCCAGCCTGTACCCGGGTGGTGTGGGAATACCTGGGc             <  2:1093948/150‑1 (MQ=255)
                                                                                                                                  gTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAGCGGCGACCGGCGCAACCCAGCCTGTACCCGGGTGGTGTGGGAATACCTGGGCga           <  1:800875/150‑1 (MQ=255)
                                                                                                                                   tCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTa                                                                      >  2:819214/1‑90 (MQ=255)
                                                                                                                                   tCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTa                                                                      <  1:819214/90‑1 (MQ=255)
                                                                                                                                     gCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAGCGGCGACCGGCGCAACCCAGCCTGTACCCGGGTGGTGTGGGAATACCTGGGCgagga        <  2:426799/150‑1 (MQ=255)
                                                                                                                                           gTTCAACGAAATGAGCCAGACCATCCTCATCCCCa                                                                                                                     >  1:514964/1‑35 (MQ=255)
                                                                                                                                           gTTCAACGAAATGAGCCAGACCATCCTCATCCCCa                                                                                                                     <  2:514964/35‑1 (MQ=255)
                                                                                                                                           gTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAGCGGCGACCGGCGCAACCCAGCCTGTACCCGGGTGGTGTGGGAATACCTGCGCGAGgagacgaa  >  2:558574/1‑146 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GCCGTTTTCTGTTCGAGCGCCAGGAACGGCAGGAGCGCTCCAGCGCCACCGATCAGCATGCCGAGGTACGCTGCCGCTACCTGGGGGCGGCGTTCCAGCTGCAGGACCGGATGTTCCTCATCGACTACGAGTCGCTGACGTTCAACGAAATGAGCCAGACCATCCTCATCCCCAGCTTCAAAAGCCGCATTACCCGGCTGAATGGCTTGAAGGCCGGCGTATCCAGCGGCGACCGGCGCAACCCAGCCTGTACCCGGGTGGTGTGGGAATACCTGGGCGAGGAGATCAA  >  NC_002947/587352‑587640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑