Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 644,549 C→G 8.2% E157D (GAG→GAC)  acoA ← acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947644,5490CG 8.2% 134.4 / 2.8 47E157D (GAG→GAC) acoAacetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (21/21);  new base G (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

ATGGTGACGCCTGGCATGCCGAAGCCGGCAGCGCGGTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTC  >  NC_002947/644400‑644698
                                                                                                                                                     |                                                                                                                                                     
aTGGTGACGCCTGGCATGCCGAAGCCGGCAGCGCGGTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGcc                                                                                                                                                       >  2:813508/1‑150 (MQ=255)
     gACGCCTGGCATGCCGAAGCCGGCAGCGCGGTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGaa                                                                                                                                                  <  1:1169495/150‑1 (MQ=255)
     gACGCCTGGCATGCCGAAGCCGGCAGCGCGGTCGACAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGaa                                                                                                                                                  <  2:611077/150‑1 (MQ=255)
       cGCCTGGCATGCCGAAGCCGGCAGCGCGGTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGANCATCCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATa                                                                                                                                                <  1:198487/150‑1 (MQ=255)
        gCCTGGCATGCCGAAGCCGGCAGCGCGGTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTc                                                                                                                                                     <  2:420362/144‑1 (MQ=255)
        gCCTGGCATGCCGAAGCCGGCAGCGCGGTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTc                                                                                                                                                     >  1:420362/1‑144 (MQ=255)
                 gCCGAAGCCGGCAGCGCGGTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTc                                                                                                                                      >  1:892990/1‑150 (MQ=255)
                  ccGAAGCCGGCAGCGCGGTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATa                                                                                                                                                >  1:514639/1‑139 (MQ=255)
                  ccGAAGCCGGCAGCGCGGTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATa                                                                                                                                                <  2:514639/139‑1 (MQ=255)
                    gAAGCCGGCAGCGCGGTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGtt                                                                                                                                   <  1:707395/150‑1 (MQ=255)
                     aaGCCGGCAGCGCGGTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTg                                                                                                                                  <  2:1170490/150‑1 (MQ=255)
                               cgcgGTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCAt                                                                                                                        >  1:506857/1‑150 (MQ=255)
                                  ggTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGACCGACGCCATGTTCATTGCCTCGAATACGGCGCCCTCGTGTTAGGCCCCATCGc                                                                                                                     >  1:1151605/1‑150 (MQ=255)
                                      ggCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGtt                                                                                                                                   >  2:318941/1‑132 (MQ=255)
                                      ggCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGtt                                                                                                                                   <  1:318941/132‑1 (MQ=255)
                                           tgtgGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAgg                                                                                                            <  2:506857/150‑1 (MQ=255)
                                            gtgGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGc                                                                                                           <  1:839085/150‑1 (MQ=255)
                                            gtgGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGc                                                                                                           <  2:892990/150‑1 (MQ=255)
                                              gggcGCAGGCTAGGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGTAGGGCAGGTTCATGATCGACGCCATGTTCATGGCGTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTa                                                                                                         <  1:221912/147‑1 (MQ=255)
                                               gTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTAc                                                                                                        <  1:1033091/150‑1 (MQ=255)
                                                    aGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGaca                                                                                                   >  1:888291/1‑150 (MQ=255)
                                                           ggACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATAGCTCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATc                                                                                            >  1:1168531/1‑150 (MQ=255)
                                                            gACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAgg                                                                                                            <  2:88842/133‑1 (MQ=255)
                                                            gACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAgg                                                                                                            >  1:88842/1‑133 (MQ=255)
                                                                 gTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGc                                                                                                                             <  1:818278/111‑1 (MQ=255)
                                                                 gTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGc                                                                                                                             >  2:818278/1‑111 (MQ=255)
                                                                     gAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCg                                                                                                                   <  2:179645/117‑1 (MQ=255)
                                                                     gAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCg                                                                                                                   >  1:179645/1‑117 (MQ=255)
                                                                       ggCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAg                                                                                <  1:813508/150‑1 (MQ=255)
                                                                              gCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCTCGGCCCTTGAGCTTGgcc                                                                         >  1:295084/1‑150 (MQ=255)
                                                                              gCCTCGGCGTAGCCGTTGTTCTCGGCCACGAACATCCAGACGCGCTTCCTTATCCACGCCATGTTCATGGCTTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGgcc                                                                         >  1:1114699/1‑150 (MQ=255)
                                                                                 tCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGgccgcc                                                                      >  1:246346/1‑150 (MQ=255)
                                                                                   ggCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGcc                                                                                                                    <  1:536068/102‑1 (MQ=255)
                                                                                   ggCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGcc                                                                                                                    >  2:536068/1‑102 (MQ=255)
                                                                                          ccGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCgccgcc                                                             >  1:1003307/1‑150 (MQ=255)
                                                                                               gttCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGc                                                        >  2:510256/1‑150 (MQ=255)
                                                                                                       ccACGAAGATGCAGGGGAGGTTCATGATGGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAgtg                                                <  2:736429/150‑1 (MQ=255)
                                                                                                        gaagAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAgtgg                                               >  2:1038799/4‑150 (MQ=255)
                                                                                                         aCGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAgtggt                                              >  1:101002/1‑150 (MQ=255)
                                                                                                             aGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAATGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAGTGGTgcgc                                          <  2:128215/150‑1 (MQ=255)
                                                                                                             aGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAGTGGTgcgc                                          <  2:246346/150‑1 (MQ=255)
                                                                                                              gATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCGTCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAGTGGTgcgcc                                         <  1:510256/150‑1 (MQ=255)
                                                                                                                              gtattCGAGGCCATGAACATGGCGTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAGTGGTGCGCCGGCGCCGACGATACCg                         >  2:888044/5‑150 (MQ=255)
                                                                                                                               tGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAGTGGTGCGCCGGCGCCGACGATACCGt                        >  2:491633/1‑150 (MQ=255)
                                                                                                                                  tCGAGGCCATGAACATGGCGTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAGTGGTGCGCCGGCGCCGACGATACCGTTgg                     >  1:341409/1‑150 (MQ=255)
                                                                                                                                          atgaacATGGCGTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGc             <  2:341409/145‑1 (MQ=255)
                                                                                                                                          aTGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGc             >  2:719727/1‑150 (MQ=255)
                                                                                                                                              tCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGc         >  1:520296/1‑150 (MQ=255)
                                                                                                                                                    ccTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCtttt   >  2:309237/1‑150 (MQ=255)
                                                                                                                                                     cTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTc  <  2:1003307/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGGTGACGCCTGGCATGCCGAAGCCGGCAGCGCGGTCGGCAATGTGGTCGCAGGCTACGGACCAGTTGGAGGCAGTGGCCTCGGCGTAGCCGTTGTTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCCACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTC  >  NC_002947/644400‑644698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑