Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 648,250 A→T 7.6% intergenic (+16/+75) acoR → / ← accC acetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947648,2500AT 7.6% 150.2 / 4.8 53intergenic (+16/+75)acoR/accCacetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base A (22/26);  new base T (2/2);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

GCGCTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACG  >  NC_002947/648115‑648396
                                                                                                                                       |                                                                                                                                                  
gcgcTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGAtt                                                                                                                                      >  1:555848/1‑150 (MQ=255)
 cgcTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTc                                                                                                                                     >  2:703544/1‑150 (MQ=255)
 cgcTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTc                                                                                                                                     <  1:436992/150‑1 (MQ=255)
  gcTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGc                                                                                                                                              >  2:638264/1‑140 (MQ=255)
  gcTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGc                                                                                                                                              <  1:638264/140‑1 (MQ=255)
   cTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTg                                                                                                                                   <  2:162991/150‑1 (MQ=255)
   cTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTg                                                                                                                                   <  1:641278/150‑1 (MQ=255)
      aTCCGCCATCGCTGGAAGGCGGCTGATGCGGCACGGGCTTTGGGGATGTCGCGGGCGACGCTTTATCGGCGAGTGCATGGGCACCGCATCGACATGCCGAGGATGCAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTggca                                                                                                                                <  1:789321/150‑1 (MQ=255)
       tCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACGTGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTggcag                                                                                                                               >  1:1146703/1‑150 (MQ=255)
            cATCGCTGGAAGCCGGCTGATGCCGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGCGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAAt                                                                                                                          <  1:549334/150‑1 (MQ=255)
                gCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCATATTCTGGCAGGCAATaaaa                                                                                                                      <  2:1043256/150‑1 (MQ=255)
                     aaGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAAccc                                                                                                                 >  1:763540/1‑150 (MQ=255)
                       gCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCg                                                                                                               >  2:308119/1‑150 (MQ=255)
                          ggCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGca                                                                                                            >  2:42868/1‑150 (MQ=255)
                           gCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGcac                                                                                                           >  1:1131963/1‑150 (MQ=255)
                             tGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAg                                                                                                         <  1:703544/150‑1 (MQ=255)
                                   ggCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCAACGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTAAAAAAAAAAAAAAAACCCCGGCACAGGGCCgg                                                                                                   <  2:555848/150‑1 (MQ=255)
                                        gggCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTc                                                                                              >  2:505755/1‑150 (MQ=255)
                                          gCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCGCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTg                                                                                            <  2:257441/150‑1 (MQ=255)
                                          gCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACCGCGCCGGGGTTCTg                                                                                            >  2:821950/1‑150 (MQ=255)
                                           cTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTgg                                                                                           <  2:763540/150‑1 (MQ=255)
                                            tttGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAAcccc                                                                                                                <  1:546168/128‑1 (MQ=255)
                                            tttGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAAcccc                                                                                                                >  2:546168/1‑128 (MQ=255)
                                            tttGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCt                                                                                             <  1:956347/147‑1 (MQ=255)
                                            tttGGGCACTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCt                                                                                             >  2:956347/1‑147 (MQ=255)
                                               gggCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACa                                                                                       <  2:334945/150‑1 (MQ=255)
                                                                  cTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGcccc                                                                                                                                           >  1:320660/1‑79 (MQ=255)
                                                                  cTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGcccc                                                                                                                                           <  2:320660/79‑1 (MQ=255)
                                                                       tCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGGTGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCCGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGt                                                               >  1:978563/1‑150 (MQ=255)
                                                                                gCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCg                                                                                                    <  2:817833/104‑1 (MQ=255)
                                                                                gCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCg                                                                                                    >  1:817833/1‑104 (MQ=255)
                                                                                    gAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTtgtg                                                  >  1:406616/1‑150 (MQ=255)
                                                                                         ccGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCCCAGGGCCGGGGTTCTGGTCCATCCCACTACACGATCACTCCTGGTTCGCCAGTTTGTGCTCGa                                             <  2:409985/150‑1 (MQ=255)
                                                                                              tCGACGTGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAg                                        <  2:1146703/150‑1 (MQ=255)
                                                                                              tCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAg                                        <  2:406616/150‑1 (MQ=255)
                                                                                               cGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGt                                       <  1:256684/150‑1 (MQ=255)
                                                                                               cGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGt                                       <  1:505755/150‑1 (MQ=255)
                                                                                               cGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGt                                       <  1:513507/150‑1 (MQ=255)
                                                                                                  cATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGaa                                    <  2:280751/150‑1 (MQ=255)
                                                                                                   aTGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGCCGCGGCCACAGCTTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAAt                                   <  2:1040991/150‑1 (MQ=255)
                                                                                                     gCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGt                                 <  2:1131963/150‑1 (MQ=255)
                                                                                                        gcgGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGa                              <  1:42868/150‑1 (MQ=255)
                                                                                                            aTGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGACTGTTGAcgcc                          >  2:370928/1‑150 (MQ=255)
                                                                                                                   ggTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTg                   <  1:299683/150‑1 (MQ=255)
                                                                                                                         aatcTGGGGCCTCGTCGCGGCCCCAGGTTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGttt                                                     <  1:676058/107‑1 (MQ=255)
                                                                                                                         aatcTGGGGCCGCGTCGCGGCCCCAGGTTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGttt                                                     >  2:676058/4‑110 (MQ=255)
                                                                                                                          cccTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGc            >  1:784916/1‑150 (MQ=255)
                                                                                                                           ccTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGcc           >  2:297042/1‑150 (MQ=255)
                                                                                                                            cTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCt          >  2:853232/1‑150 (MQ=255)
                                                                                                                             tGGGGCCGCGTCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTgg                                                                                           >  1:870427/1‑68 (MQ=255)
                                                                                                                             tGGGGCCGCGTCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTgg                                                                                           <  2:870427/68‑1 (MQ=255)
                                                                                                                                  ccGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGGTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTtcatcc    >  1:9230/1‑149 (MQ=255)
                                                                                                                                    gcgACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACg  >  1:1056215/1‑150 (MQ=255)
                                                                                                                                       |                                                                                                                                                  
GCGCTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACG  >  NC_002947/648115‑648396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑