Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	671,784	T→A	8.2%	L279Q (CTG→CAG)	PP_0573 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	671,784	0	T	A	8.2%	176.8 / 2.6	59	L279Q (CTG→CAG)	PP_0573	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/26);  new base A (2/3);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCAGCTGACCGTGCATGCGCGGCGCAAGCAGCTCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACGATGGCC  >  NC_002947/671640‑671924                                                                                                                                                 |                                                                                                                                             cccAGCTGACCGTGCATGCGCGGCGCAAGCAGCTCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCtgctgc                                                                                                                                         >  2:454537/1‑150 (MQ=255)     gCTGACCGTGCATGCGCGGCGCAAGCAGCTCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGccccc                                                                                                                                     >  1:1059934/1‑150 (MQ=255)      cTGACCGTGCATGCGCGGCGCAAGCAGCTCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCt                                                                                                                                    <  2:483972/150‑1 (MQ=255)        gACCGTGCATGCGCGGCGCAAGCAGCTCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTgg                                                                                                                                  <  1:686180/150‑1 (MQ=255)        gACCGTGCATGCGCGGCGCAAGCAGCTCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTgg                                                                                                                                  <  1:454537/150‑1 (MQ=255)           cGTGCATGCGCGGCGCAAGCAGCTCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCt                                                                                                                                    >  1:1133163/1‑145 (MQ=255)           cGTGCATGCGCGGCGCAAGCAGCTCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCt                                                                                                                                    <  2:1133163/145‑1 (MQ=255)              gCATGCGCGGCGCAAGCAGCTCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGa                                                                                                                            >  1:1037710/1‑150 (MQ=255)                            agcagcTCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTAcc                                                                                                              >  2:1062199/1‑150 (MQ=255)                             gcagcTCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCa                                                                                                             >  2:1099068/1‑150 (MQ=255)                                 cTCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCCTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCCATGACCGGGTCTATTACCAgcgc                                                                                                         >  2:292625/1‑150 (MQ=255)                                  tCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGcc                                                                                                        >  1:820834/1‑150 (MQ=255)                                     gcgcCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCg                                                                                                     >  2:1087842/1‑150 (MQ=255)                                      cgccgcTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGa                                                                                                    <  1:856322/150‑1 (MQ=255)                                           cTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACa                                                                                               <  1:277741/150‑1 (MQ=255)                                            tgtgACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAg                                                                                              >  2:713862/1‑150 (MQ=255)                                             gtgACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGc                                                                                             <  1:1087842/150‑1 (MQ=255)                                                aCCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGcccc                                                                                                                                      >  1:427751/1‑106 (MQ=255)                                                aCCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGcccc                                                                                                                                      <  2:427751/106‑1 (MQ=255)                                                  cGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTa                                                                                        <  1:657690/150‑1 (MQ=255)                                                   gTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTgg                                                                                                                                  <  2:770009/107‑1 (MQ=255)                                                   gTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTgg                                                                                                                                  >  1:770009/1‑107 (MQ=255)                                                   gTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCgg                                                                                                                        >  2:1174145/1‑117 (MQ=255)                                                   gTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCgg                                                                                                                        <  1:1174145/117‑1 (MQ=255)                                                        cGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGa                                                                                  >  2:807693/1‑150 (MQ=255)                                                         gACTCCAGCCGTGATGATGAACAGGACCTGATCACTGTGGCCAGCTGTGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGAc                                                                                 <  2:1037710/150‑1 (MQ=255)                                                                     gATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCgg                                                                         <  1:489434/146‑1 (MQ=255)                                                                     gATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCgg                                                                         >  2:489434/1‑146 (MQ=255)                                                                        gctgcACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCaa                                                                  <  1:897598/150‑1 (MQ=255)                                                                        gctgcACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCaa                                                                  >  1:757459/1‑150 (MQ=255)                                                                          tgcACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAAct                                                                <  2:820834/150‑1 (MQ=255)                                                                             acaGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAActgct                                                             <  1:1062199/150‑1 (MQ=255)                                                                              caGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAActgctg                                                            <  1:1099068/150‑1 (MQ=255)                                                                                    cTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCg                                                      >  1:5233/1‑150 (MQ=255)                                                                                     tGATCACCGTAGCCAGCAATGCACAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCgg                                                     >  2:833614/1‑150 (MQ=255)                                                                                           ccGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTcgc                                               >  1:122572/1‑150 (MQ=255)                                                                                           ccGTAGCCACCCATGCCCAGACCGGCCATGGCTAACGCGTCACGTCGAGCTGGCTGCTGCCCCCTGGCAATTACCGTGTCTATTACCAGCACCTCGCTTACAGCGAGTACCTTGACGACGTCCGTAGCCAACTGCTGCTGTCGGCGTcgc                                               <  2:241189/150‑1 (MQ=255)                                                                                            cGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTcgcg                                              >  2:1105989/1‑150 (MQ=255)                                                                                              tAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCCGCCTGCGGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGCt                                            <  1:1168175/150‑1 (MQ=255)                                                                                                   aGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTNCTGCCCCCTGGCAATGACCGGGTCTAATACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAGCTGCTGCTGTCGGCGTCGCGCAACCGc                                       >  1:358830/1‑150 (MQ=255)                                                                                                   aGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGCTACCGc                                       >  2:1109790/1‑150 (MQ=255)                                                                                                   aGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGATAGCCAACTGCTGCTGTCg                                                      <  1:114393/135‑1 (MQ=255)                                                                                                   aGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGATAGCCAACTGCTGCTGTCg                                                      >  2:114393/1‑135 (MQ=255)                                                                                                     cAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGCTACCGCAg                                     >  1:227165/1‑150 (MQ=255)                                                                                                           ccAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGCTACCGCAGCGAGcc                               >  1:908205/1‑150 (MQ=255)                                                                                                                      aTGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGAc                                                                                 >  2:839308/1‑89 (MQ=255)                                                                                                                      aTGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGAc                                                                                 <  1:839308/89‑1 (MQ=255)                                                                                                                      aTGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGGTACCGCAGCGAGCCCGGCACGCGCa                    >  1:469748/1‑150 (MQ=255)                                                                                                                       aatagacccggtcattGCCAGGGGGCAGCAGCCAGCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAActgctgc                                                           >  1:17258‑M1/17‑110 (MQ=255)                                                                                                                       aatagacccggtcattGCCAGGGGGCAGCAGCCAGCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAActgctgc                                                           <  2:17258‑M1/94‑1 (MQ=255)                                                                                                                           gAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGt               <  2:704871/150‑1 (MQ=255)                                                                                                                            acccggtcattGCCAGGGGGCAGCAGCCAGCTGGCAATGCCCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGCTACCGCAGCGAGCCCGGCAcgc                       >  1:601592‑M1/12‑141 (MQ=255)                                                                                                                            acccggtcattGCCAGGGGGCAGCAGCCAGCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGCTACCGCAGCGAGCCCGGCAcgc                       <  2:601592‑M1/130‑1 (MQ=255)                                                                                                                                cgTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGa          <  1:943673/150‑1 (MQ=255)                                                                                                                                  tcattGCCAGGGGGCAGCAGCCAGCTGGCAATGAGCGGGTCTATTACCAGCGCAGCGATTACAGCCAGTAGCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTTGCGCTACCGCAGCGGGCCCGGCACGCGCATACGTTTGGACg        <  2:367181‑M1/145‑1 (MQ=255)                                                                                                                                  tCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTa                                                                       >  2:717393/1‑87 (MQ=255)                                                                                                                                  tCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTa                                                                       <  1:717393/87‑1 (MQ=255)                                                                                                                                  tCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACg        >  1:1139494/1‑150 (MQ=255)                                                                                                                                    aCGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACGAt      <  2:594121/150‑1 (MQ=255)                                                                                                                                        CCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACGATGGcc  <  1:865454‑M1/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                             CCCAGCTGACCGTGCATGCGCGGCGCAAGCAGCTCAGCGCCGCTGTGACCGTGGCCGACTCCAGCCGTGATGCTGCACAGGCCCTGATCACCGTAGCCAGCAATGCCCAGACCCGCCATGGCGAACGCGTCACGGCCAGCTGGCTGCTGCCCCCTGGCAATGACCGGGTCTATTACCAGCGCCTCGATTACAGCCAGTACCTGGACGCCGCCGGTAGCCAACTGCTGCTGTCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACGATGGCC  >  NC_002947/671640‑671924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑