Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 682,315 G→T 10.3% P321T (CCG→ACG)  PP_0584 ← methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947682,3150GT10.3% 110.7 / 3.1 39P321T (CCG→ACG) PP_0584methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base G (18/17);  new base T (2/2);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

CTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAGGTCCAG  >  NC_002947/682166‑682457
                                                                                                                                                     |                                                                                                                                              
cTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCgg                                                                                                                                                >  1:675441/1‑150 (MQ=255)
cTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCgg                                                                                                                                                >  1:1145025/1‑150 (MQ=255)
    aTCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGgcgc                                                                                                                                            >  1:723435/1‑150 (MQ=255)
          cTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATc                                                                                                                                      >  1:1069804/1‑150 (MQ=255)
          cTGCGAATGGCACCGAGGAACTGGTTGAACCAGATGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATc                                                                                                                                      >  1:699135/1‑150 (MQ=255)
               aaTGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGTGCGCCCGATCaacag                                                                                                                                 <  1:94130/150‑5 (MQ=255)
                         aGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGcc                                                                                                                       >  1:486932/1‑150 (MQ=255)
                                ggTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGa                                                                                                                <  2:604006/150‑1 (MQ=255)
                                 gTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGac                                                                                                               >  1:331300/1‑150 (MQ=255)
                                     aaCCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGaccacc                                                                                                           >  1:748009/1‑150 (MQ=255)
                                            tGGCCAGTTGCGCGTTTTCGTCGCGGCCGCGGAGTTCCAGGTTGCGGGTCAGGTCACTCTCACCTTGGGAAATGTCTTCCAGGCCATTGGTGACGCTGTTGACCGGGCGCACGATCAGTTTGGCTAATGCCGCACCGCCCACCGCGAACa                                                                                                    <  2:539363/150‑1 (MQ=255)
                                                               gtcgCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGc                                                                                 >  2:361589/1‑150 (MQ=255)
                                                                                   ggTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGGGAGGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGACGCACCGACCACCGCGAGCGGCGCGGCCAGCACAACAGCGATGACACTGATCAGCCAgg                                                             >  2:191898/1‑150 (MQ=255)
                                                                                                gtcaCCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAg                                                              >  2:358666/1‑136 (MQ=255)
                                                                                                gtcaCCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAg                                                              <  1:358666/136‑1 (MQ=255)
                                                                                                gtcaCCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGggtggt                                                >  1:212501/1‑150 (MQ=255)
                                                                                                 tcaCCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAATAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGggtggtg                                               <  2:1069804/150‑1 (MQ=255)
                                                                                                    cccTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTc                                            <  2:331300/150‑1 (MQ=255)
                                                                                                     ccTCACCTTGGGCAATGTCTTCCAGGCCATTGGTCTCGCTGTTGATCGGCCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCt                                           <  2:699135/150‑1 (MQ=255)
                                                                                                     ccTCACCGTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCt                                           <  2:1143416/150‑1 (MQ=255)
                                                                                                       tCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACCGCGATGACCCCGATAAGCCAGGTGAGCCGGGTGGTGGTCTGc                                         >  2:325504/1‑150 (MQ=255)
                                                                                                         aCCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCAt                                       <  1:361589/150‑1 (MQ=255)
                                                                                                           cTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGa                                                                      <  2:199616/117‑1 (MQ=255)
                                                                                                           cTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGa                                                                      >  1:199616/1‑117 (MQ=255)
                                                                                                           cTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCa                                     <  2:1097973/150‑1 (MQ=255)
                                                                                                           cTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATAGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCa                                     <  1:325504/150‑1 (MQ=255)
                                                                                                            ttGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCAc                                    >  2:1087410/1‑150 (MQ=255)
                                                                                                                  aaTGTCTTCCCGGCCATTGGTGACGCTGTTCATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCt                              <  2:672782/150‑1 (MQ=255)
                                                                                                                     gTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCgg                                                     >  1:518308/1‑124 (MQ=255)
                                                                                                                     gTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCgg                                                     <  2:518308/124‑1 (MQ=255)
                                                                                                                        ttCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTc                        >  2:727346/1‑150 (MQ=255)
                                                                                                                            aGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGc                                         <  2:1108217/129‑1 (MQ=255)
                                                                                                                            aGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGc                                         >  1:1108217/1‑129 (MQ=255)
                                                                                                                                        aCGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCCCCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAg        <  2:723435/150‑1 (MQ=255)
                                                                                                                                         ccaaactGATCGTGCGCCCGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAgg       >  1:483969/7‑150 (MQ=255)
                                                                                                                                          gCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCa                                        <  2:1136842/116‑1 (MQ=255)
                                                                                                                                          gCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCa                                        >  1:1136842/1‑116 (MQ=255)
                                                                                                                                          gCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAGGt      >  2:267523/1‑150 (MQ=255)
                                                                                                                                          gCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAGGt      <  1:1087410/150‑1 (MQ=255)
                                                                                                                                             actGATCGTGCGCCCGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAGGTcca   >  1:180699/3‑150 (MQ=255)
                                                                                                                                              ctGATCGTGCGCCCGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAGGTccag  <  2:483969/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
CTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAGGTCCAG  >  NC_002947/682166‑682457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑