Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 682,320 A→C 10.6% V319G (GTG→GGG)  PP_0584 ← methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947682,3200AC10.6% 86.0 / 5.3 38V319G (GTG→GGG) PP_0584methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base A (17/17);  new base C (2/2);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

CTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAGGTCCAG  >  NC_002947/682176‑682457
                                                                                                                                                |                                                                                                                                         
cTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATc                                                                                                                                      >  1:1069804/1‑150 (MQ=255)
cTGCGAATGGCACCGAGGAACTGGTTGAACCAGATGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATc                                                                                                                                      >  1:699135/1‑150 (MQ=255)
     aaTGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGTGCGCCCGATCaacag                                                                                                                                 <  1:94130/150‑5 (MQ=255)
               aGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGcc                                                                                                                       >  1:486932/1‑150 (MQ=255)
                      ggTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGa                                                                                                                <  2:604006/150‑1 (MQ=255)
                       gTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGac                                                                                                               >  1:331300/1‑150 (MQ=255)
                           aaCCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGaccacc                                                                                                           >  1:748009/1‑150 (MQ=255)
                                  tGGCCAGTTGCGCGTTTTCGTCGCGGCCGCGGAGTTCCAGGTTGCGGGTCAGGTCACTCTCACCTTGGGAAATGTCTTCCAGGCCATTGGTGACGCTGTTGACCGGGCGCACGATCAGTTTGGCTAATGCCGCACCGCCCACCGCGAACa                                                                                                    <  2:539363/150‑1 (MQ=255)
                                                     gtcgCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGc                                                                                 >  2:361589/1‑150 (MQ=255)
                                                                         ggTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGGGAGGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGACGCACCGACCACCGCGAGCGGCGCGGCCAGCACAACAGCGATGACACTGATCAGCCAgg                                                             >  2:191898/1‑150 (MQ=255)
                                                                                      gtcaCCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAg                                                              >  2:358666/1‑136 (MQ=255)
                                                                                      gtcaCCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAg                                                              <  1:358666/136‑1 (MQ=255)
                                                                                      gtcaCCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGggtggt                                                >  1:212501/1‑150 (MQ=255)
                                                                                       tcaCCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAATAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGggtggtg                                               <  2:1069804/150‑1 (MQ=255)
                                                                                          cccTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTc                                            <  2:331300/150‑1 (MQ=255)
                                                                                           ccTCACCTTGGGCAATGTCTTCCAGGCCATTGGTCTCGCTGTTGATCGGCCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCt                                           <  2:699135/150‑1 (MQ=255)
                                                                                           ccTCACCGTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCt                                           <  2:1143416/150‑1 (MQ=255)
                                                                                             tCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACCGCGATGACCCCGATAAGCCAGGTGAGCCGGGTGGTGGTCTGc                                         >  2:325504/1‑150 (MQ=255)
                                                                                               aCCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCAt                                       <  1:361589/150‑1 (MQ=255)
                                                                                                 cTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGa                                                                      >  1:199616/1‑117 (MQ=255)
                                                                                                 cTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGa                                                                      <  2:199616/117‑1 (MQ=255)
                                                                                                 cTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCa                                     <  2:1097973/150‑1 (MQ=255)
                                                                                                 cTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATAGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCa                                     <  1:325504/150‑1 (MQ=255)
                                                                                                  ttGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCAc                                    >  2:1087410/1‑150 (MQ=255)
                                                                                                        aaTGTCTTCCCGGCCATTGGTGACGCTGTTCATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCt                              <  2:672782/150‑1 (MQ=255)
                                                                                                           gTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCgg                                                     >  1:518308/1‑124 (MQ=255)
                                                                                                           gTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCgg                                                     <  2:518308/124‑1 (MQ=255)
                                                                                                              ttCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTc                        >  2:727346/1‑150 (MQ=255)
                                                                                                                  aGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGc                                         <  2:1108217/129‑1 (MQ=255)
                                                                                                                  aGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGc                                         >  1:1108217/1‑129 (MQ=255)
                                                                                                                              aCGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCCCCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAg        <  2:723435/150‑1 (MQ=255)
                                                                                                                               ccaaactGATCGTGCGCCCGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAgg       >  1:483969/7‑150 (MQ=255)
                                                                                                                                gCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCa                                        <  2:1136842/116‑1 (MQ=255)
                                                                                                                                gCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCa                                        >  1:1136842/1‑116 (MQ=255)
                                                                                                                                gCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAGGt      >  2:267523/1‑150 (MQ=255)
                                                                                                                                gCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAGGt      <  1:1087410/150‑1 (MQ=255)
                                                                                                                                   actGATCGTGCGCCCGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAGGTcca   >  1:180699/3‑150 (MQ=255)
                                                                                                                                    ctGATCGTGCGCCCGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAGGTccag  <  2:483969/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                         
CTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCGCACCGACCACCGCGAACAGCGCGGCCAACACCACAGCGATGACACCGATCAGCCAGGTGAGCCGGGTGGTGGTCTGCATCACTTCGCTTTGTTCGATCAGGCCGATGAAGGTCCAG  >  NC_002947/682176‑682457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑