| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 912,426 | G→T | 8.1% | Q26H (CAG→CAT) | fruK → | 1‑phosphofructokinase monomer |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 912,426 | 0 | G | T | 8.1% | 155.7 / 2.8 | 49 | Q26H (CAG→CAT) | fruK | 1‑phosphofructokinase monomer |
| Reads supporting (aligned to +/- strand): ref base G (17/28); new base T (2/2); total (19/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
CTGGCGCGCGAGGCCCTGCAACAGGACAGCGCCGCAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTGGGGCACAGCGTAACCGTGGGCGGTTTCCTGGGGGGCGACAATCTGCAGCCCTTCGAGGCGCTGATCGACG > NC_002947/912283‑912571 | cTGGCGCGCGAGGCCCTGCAACAGGACAGCGCCGCAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACACTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAAc < 2:674407/150‑1 (MQ=255) cgcgAGGCCCTGCAACAGGACAGCGCCGCAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGc < 1:152452/150‑1 (MQ=255) gcgAGGCCCTGCAACAGGACAGCGCCGCAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGcc > 1:497582/1‑150 (MQ=255) gAGGCCCTGCAACAGGACAGCGCCGCAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAg < 2:453409/150‑1 (MQ=255) ccTGCAACAGGACAGCGCCGCAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCgca > 2:132190/1‑150 (MQ=255) ccTGCAACAGGACAGCGCCGCAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCgca > 1:7191/1‑150 (MQ=255) gCAACAGGACAGCGCCGCAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCgcagca > 1:52641/1‑150 (MQ=255) aaCAGGACAGCGCCGCAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGcaca > 2:975928/1‑150 (MQ=255) aCAGGACAGCGCCGCAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAg < 2:1030599/150‑1 (MQ=255) cAGGACAGCGCCGCAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGt < 1:747816/150‑1 (MQ=255) cAGCGCCGCAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCAcgc < 1:1128269/150‑1 (MQ=255) cgcAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAgg < 1:923380/150‑1 (MQ=255) ttCGCGCGCTGGTGGAGCGTTACTGCACCGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGc < 1:907594/150‑1 (MQ=255) gcgcTGGTGGAGCGTTACTGAATGGCCAATATCCTCACTCTCACCCTGAACCCTGCTCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAAc < 2:415070/150‑1 (MQ=255) tggtggAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGGCCAGGGCAGGTCAACCGTAGCCCCGCGCAGCACAGTCACGCCGCAGGCGAGGGCCTTAACGTTg < 2:777139/150‑1 (MQ=255) ggtggAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATTTCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGc < 2:658133/150‑1 (MQ=255) gtggAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGcc > 2:771328/1‑150 (MQ=255) ggAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCa < 2:497582/150‑1 (MQ=255) ttACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACt < 1:132190/150‑1 (MQ=255) aCTGAATGGCCAAGTCCCTCAGTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGAGCAGCACAGTCACCCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTgg < 1:394385/150‑1 (MQ=255) tGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTCGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGcc < 1:751556/150‑1 (MQ=255) tGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGAcc < 1:771328/150‑1 (MQ=255) cgCCAAGATCCTCACTCTCACCCTGCACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCCCAGTCACGCCGAAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCt < 2:471788/149‑1 (MQ=255) ccAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTgg < 2:1094921/150‑1 (MQ=255) tCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTGGGGCACAGCg > 2:353747/1‑150 (MQ=255) ccTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTGGGGCACAGCGTAACCGTggg > 2:1100358/1‑150 (MQ=255) ccTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTGGGGCACAGCGTAACCGTGGGCGGTTTc < 1:471775/150‑1 (MQ=255) tGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGccc > 1:1191529/1‑104 (MQ=255) tGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGccc < 2:1191529/104‑1 (MQ=255) ggATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTGGGGCACAGCGTAACCGTGGGCGGTTTCCTgggggg < 1:1100358/150‑1 (MQ=255) tatCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTGGGGCACAGCGTAACCGTGGGCGGTTTCCTGGGGGGCGa < 2:863119/150‑1 (MQ=255) cACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTGGGGcaca > 2:34420/1‑116 (MQ=255) cACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTGGGGcaca < 1:34420/116‑1 (MQ=255) aCCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGc < 2:910130/101‑1 (MQ=255) aCCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGc > 1:910130/1‑101 (MQ=255) aCCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTGGGGCGCAGCGTAACCGTGGGCGGTTTCCTGGGGGGCGACAAt < 1:975928/150‑1 (MQ=255) aCCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTGGGGCACAGCGTAACCGTGGGCGGTTTCCTGGGGGGCGACAAt > 1:836472/1‑150 (MQ=255) tCGGCCTGGACACTCTGCGCCCAGGGCATGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTgg > 2:1205693/1‑105 (MQ=255) tCGGCCTGGACACTCTGCGCCCAGGGCATGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTgg < 1:1205584/105‑1 (MQ=255) tCGGCCTGGACACTCTGCGCCCAGGGCATGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTgg > 2:1205584/1‑105 (MQ=255) tCGGCCTGGACACTCTGCGCCCAGGGCATGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCCgg < 1:1205693/105‑1 (MQ=255) tCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAgggg > 1:414298/1‑74 (MQ=255) tCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAgggg < 2:414298/74‑1 (MQ=255) ggCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTGGGGCACAGCGTAACCGTGGGCGGTTTCCTGGGGGGCGACAATCTGCAg > 2:573233/1‑150 (MQ=255) tctGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCt > 1:110686/1‑90 (MQ=255) tctGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCt < 2:110686/90‑1 (MQ=255) ccAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAgggg < 1:862760/54‑1 (MQ=255) ccAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAgggg > 2:862760/1‑54 (MQ=255) ggCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTGGGGCACAGCGTAACCGTGGGCGGTTTCCTGGGGGGCGACAATCTGCAGCCCTTCGAGGCGCTGATCGACg > 1:1060376/1‑150 (MQ=255) | CTGGCGCGCGAGGCCCTGCAACAGGACAGCGCCGCAGCAGTTCGCGCGCTGGTGGAGCGTTACTGAATGGCCAAGATCCTCACTCTCACCCTGAACCCTGCGCTGGATATCACCATCGGCCTGGACACTCTGCGCCCAGGGCAGGTCAACCGTAGCCAGGCGCAGCACAGTCACGCCGCAGGCAAGGGGCTGAACGTTGCCCAGGTACTGGCCGACCTGGGGCACAGCGTAACCGTGGGCGGTTTCCTGGGGGGCGACAATCTGCAGCCCTTCGAGGCGCTGATCGACG > NC_002947/912283‑912571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |