Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 105,770 G→A 7.7% V188V (GTG→GTA)  cynT → carbonic anhydrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947105,7700GA 7.7% 145.7 / 2.5 52V188V (GTG→GTA) cynTcarbonic anhydrase
Reads supporting (aligned to +/- strand):  ref base G (23/25);  new base A (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

TGTTGGCGCCTGGTTGCGCCACGCTGAAGTGGCCCGCACCGTGGTCGAGAACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGTGAACCCGTTCCCTGCGCCACTCCGAGAGGC  >  NC_002947/105623‑105917
                                                                                                                                                   |                                                                                                                                                   
tGTTGGCGCCTGGTTGCGCCACGCTGAAGTGGCCCGCACCGTGGTCGAGAACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGc                                                                                                                                                   <  2:1196093/150‑1 (MQ=255)
     gcgcCTGGTTGCGCCACGCTGAAGTGGCCCGCACCGTGGTCGAGAACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCAAGCTGCATCACTTGCGCACCCCTCCTTCGGTGGCCTcgc                                                                                                                                              <  1:698190/150‑1 (MQ=255)
         cTGGTTGCGCCACGCTGAAGTGGCCCGCACCGTGGTCGAGAACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCt                                                                                                                                          >  2:1131714/1‑150 (MQ=255)
          tGGTTGCGCCACGCTGAAGTGGCCCGCACCGTGGTCGAGAACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAGGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTg                                                                                                                                         <  2:344789/150‑1 (MQ=255)
                 gcCACGCTGAAGTGGCCCGCACCGTGGTCGAGAACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCg                                                                                                                                  <  1:1134329/150‑1 (MQ=255)
                 gcCACGCTGAAGTGGCCCGCACCGTGGTCGAGAACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCg                                                                                                                                  <  1:846722/150‑1 (MQ=255)
                   cACGCTGAAGTGGCCCGCACCGTGGTCGAGAACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGt                                                                                                                                >  2:504333/1‑150 (MQ=255)
                     cGCTGAAGTTGCCCGCACCGTGGTCGAGAACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGa                                                                                                                              <  2:305445/150‑1 (MQ=255)
                     cGCTGAAGTGGCCCGCACCGTGGTCGAGAACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGa                                                                                                                              <  1:504333/150‑1 (MQ=255)
                                     aCCGTGGTCGAGAACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGt                                                                                                              <  1:1131714/150‑1 (MQ=255)
                                              gagaACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTg                                                                                                            >  2:849184/1‑143 (MQ=255)
                                              gagaACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTg                                                                                                            <  1:849184/143‑1 (MQ=255)
                                              gagaACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTAc                                                                                                     >  2:372219/1‑150 (MQ=255)
                                              gagaACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTAc                                                                                                     <  2:205700/150‑1 (MQ=255)
                                              gagaACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTAGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTAc                                                                                                     >  2:964874/1‑150 (MQ=255)
                                                   caacTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTAGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACAt                                                                                                <  1:964874/150‑1 (MQ=255)
                                                       tGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTCAGCTGTTCATTCATGGTTGGGTGTACGACATCgag                                                                                            >  1:5428/1‑150 (MQ=255)
                                                                       aaCACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAg                                                                            <  1:839927/150‑1 (MQ=255)
                                                                            gagaCGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTac                                                                       <  1:259951/150‑1 (MQ=255)
                                                                                      aaGTGCTGACCAAAGAGAACGTCCTCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGaa                                                                             >  2:241631/1‑134 (MQ=255)
                                                                                      aaGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGaa                                                                             <  1:241631/134‑1 (MQ=255)
                                                                                      aaGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCa                                                             >  1:1120528/1‑150 (MQ=255)
                                                                                       aGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAg                                                            <  2:910210/150‑1 (MQ=255)
                                                                                         tGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTAc                                                                                                     >  2:542007/1‑107 (MQ=255)
                                                                                         tGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTAGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTAc                                                                                                     <  1:542007/107‑1 (MQ=255)
                                                                                            tGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACa                                                       <  2:1120528/150‑1 (MQ=255)
                                                                                                cAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCtt                                                   >  2:382523/1‑150 (MQ=255)
                                                                                                          gTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGAAATCGAGACCAGCAAGATCGAAGCCTACGCCGCCGCCAGCGACCGCTTGCTGCCCttc                                         >  1:520023/1‑149 (MQ=255)
                                                                                                           tCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTgg                                        >  1:676098/1‑150 (MQ=255)
                                                                                                               cGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCcgc                                    >  1:833073/1‑150 (MQ=255)
                                                                                                                gCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGc                                                                                      <  1:944624/99‑1 (MQ=255)
                                                                                                                gCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGc                                                                                      >  2:944624/1‑99 (MQ=255)
                                                                                                                   cAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGt                                >  2:568884/1‑150 (MQ=255)
                                                                                                                         cATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGTGAAccc                          >  2:678732/1‑150 (MQ=255)
                                                                                                                          aTCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCga                                                                                             >  2:198279/1‑82 (MQ=255)
                                                                                                                          aTCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCga                                                                                             <  1:198279/82‑1 (MQ=255)
                                                                                                                           tCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCa                                                                                        <  2:831946/86‑1 (MQ=255)
                                                                                                                           tCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCa                                                                                        >  1:831946/1‑86 (MQ=255)
                                                                                                                           tCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGTGAACCCGt                        >  1:758031/1‑150 (MQ=255)
                                                                                                                           tCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGTGAACCCGt                        <  2:676098/150‑1 (MQ=255)
                                                                                                                             aCTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGTGAACCCGTTc                      <  2:5428/150‑1 (MQ=255)
                                                                                                                              cTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGTGAACCCGTTcc                     <  1:372219/150‑1 (MQ=255)
                                                                                                                               ttGCGCACCCATCCTTCGGTAGCCTCGCGCCTGGCAGCCGATGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCgg                                  <  2:794248/136‑1 (MQ=255)
                                                                                                                               ttGCGCACCCATCCTTCGGTAGCCTCGCGCCTGGCAGCCGATGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCgg                                  >  1:794248/1‑136 (MQ=255)
                                                                                                                                    cACCCATCCTGCGGTGGCGTCGCGCCTGGCAGCCGGGTAGCTGGTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGTGAACCCGTTCCGTgcgc               <  2:520023/150‑1 (MQ=255)
                                                                                                                                       ccATCCTTCGGTGGCCTCGCGCCTGGTGTCACGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGTGAACCCGTTCCCTGCGCCAc            >  1:634820/1‑150 (MQ=255)
                                                                                                                                       ccATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCCTTGGTCTTTATTTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCGTCCAACGTCAGCTTCCTGCCCTTGGCCGCGGGTGAACCCGTTCCCTGCGCCAc            >  1:937391/1‑150 (MQ=255)
                                                                                                                                       ccATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGTGAACCCGtt                       <  2:885334/139‑1 (MQ=255)
                                                                                                                                       ccATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGTGAACCCGtt                       >  1:885334/1‑139 (MQ=255)
                                                                                                                                       ccATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGTAAACCCGTTCCCTGCGCCAc            >  2:793461/1‑150 (MQ=255)
                                                                                                                                         aTCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGTGAACCCGTTCCCTGCGCCACTc          <  1:382523/150‑1 (MQ=255)
                                                                                                                                                 gTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCCGCCAGATCGAAGCCTACGGCGCCGCCAGCCACCGCTTCCTGCCCTTCGCCGCGCGCGACCCCGATCCCTGCGCCACTCCGAGAGGc  >  2:502482/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGTTGGCGCCTGGTTGCGCCACGCTGAAGTGGCCCGCACCGTGGTCGAGAACAACTGCTCCTGCGGCAGCGAACACGAGACGATGCAAGTGCTGACCAAAGAGAACGTCATCGCCCAGCTGCATCACTTGCGCACCCATCCTTCGGTGGCCTCGCGCCTGGCAGCCGGTGAGCTGTTCATTCATGGTTGGGTGTACGACATCGAGACCAGCAAGATCGAAGCCTACGACGCCGCCAGCGACAGCTTCCTGCCCTTGGCCGCGGGTGAACCCGTTCCCTGCGCCACTCCGAGAGGC  >  NC_002947/105623‑105917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑