Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 970,240 T→G 8.2% F29C (TTC→TGC) ‡ tgt → queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947970,2400TG 8.2% 125.7 / 5.2 49F29C (TTC→TGC) ‡tgtqueuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (21/24);  new base G (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

ACCCGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCGGGCACCGAGGTGATCAAGAAGCACAACGGCCTGCACGATT  >  NC_002947/970099‑970389
                                                                                                                                             |                                                                                                                                                     
aCCCGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATGAATGCCgg                                                                                                                                               <  2:192784/150‑1 (MQ=255)
 cccGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGt                                                                                                                                              <  1:631113/150‑1 (MQ=255)
   cGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTgg                                                                                                                                            >  2:72756/1‑150 (MQ=255)
      aaTCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGa                                                                                                                                         <  2:396552/150‑1 (MQ=255)
                cACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCAc                                                                                                                               >  2:344668/1‑150 (MQ=255)
                cACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCAc                                                                                                                               <  1:551885/150‑1 (MQ=255)
                            aGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCACATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCAt                                                                                                                   <  1:675655/150‑1 (MQ=255)
                              gATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCAtgc                                                                                                                 <  2:607354/150‑1 (MQ=255)
                              gATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACACCGGCATGAATGCCggggt                                                                                                                                            <  1:581064/123‑4 (MQ=255)
                              gATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACACCGGCATGAATGCCggggt                                                                                                                                            >  2:581064/1‑120 (MQ=255)
                                 cAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCAtgctgc                                                                                                              >  1:1086369/1‑150 (MQ=255)
                                        gAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGa                                                                                                       <  2:857370/150‑1 (MQ=255)
                                         aGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGAc                                                                                                      <  1:268664/150‑1 (MQ=255)
                                               aCCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAg                                                                                                >  2:1027476/1‑150 (MQ=255)
                                               aCCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCAACCGTGATCGAg                                                                                                >  1:654524/1‑150 (MQ=255)
                                               aCCTGTCGAATGTCCTTCGAACTGCTGGCCAACGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAg                                                                                                <  2:664974/150‑1 (MQ=255)
                                                 cTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCAc                                                                                                           <  1:347033/137‑1 (MQ=255)
                                                 cTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCAc                                                                                                           >  2:347033/1‑137 (MQ=255)
                                                   gTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCa                                                                                            >  2:742231/1‑150 (MQ=255)
                                                    tCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCAtg                                                                                                                  <  2:626402/127‑1 (MQ=255)
                                                    tCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCAGTCGGGGTCGCATCACCTTCCCACGTGGCACCTTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCAtg                                                                                                                  >  1:626402/1‑127 (MQ=255)
                                                      gAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGc                                                                                                                                                  <  2:126190/93‑1 (MQ=255)
                                                      gAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGc                                                                                                                                                  >  1:126190/1‑93 (MQ=255)
                                                      gAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCg                                                                                         >  2:1072104/1‑150 (MQ=255)
                                                             cTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCga                                                                                  <  1:742231/150‑1 (MQ=255)
                                                              ttCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAg                                                                                                <  1:369729/135‑1 (MQ=255)
                                                              ttCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAg                                                                                                >  2:369729/1‑135 (MQ=255)
                                                              ttCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCgag                                                                                 >  2:24697/1‑150 (MQ=255)
                                                                     tgctgGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATcc                                                                          >  1:743764/1‑150 (MQ=255)
                                                                            cACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCaa                                                                   >  2:411233/1‑150 (MQ=255)
                                                                             aCCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAac                                                                  <  2:743764/150‑1 (MQ=255)
                                                                                    gCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTcc                                                           >  1:106184/1‑150 (MQ=255)
                                                                                           ccGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCtctg                                                    <  1:1204170/150‑1 (MQ=255)
                                                                                              tCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCt                                                 <  1:411233/150‑1 (MQ=255)
                                                                                                ggggTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGc                                               <  1:1027476/150‑1 (MQ=255)
                                                                                                     cGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCg                                          <  2:1111389/150‑1 (MQ=255)
                                                                                                      gCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCgg                                         >  2:1095011/1‑150 (MQ=255)
                                                                                                               tCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTCTGGCAGAGTTCTTTAAGAACTGGCACTCGACATCGAGTCCATCGGCCCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCGGGCACCGAgg                                <  1:274900/150‑1 (MQ=255)
                                                                                                                  cACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCGGGCACCGAGGTGa                             <  1:72756/150‑1 (MQ=255)
                                                                                                                   aCGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCaa                                                                   <  1:189553/111‑1 (MQ=255)
                                                                                                                   aCGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCaa                                                                   >  2:189553/1‑111 (MQ=255)
                                                                                                                          aCCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAg                                                                                                >  2:459247/1‑75 (MQ=255)
                                                                                                                          aCCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAg                                                                                                <  1:459247/75‑1 (MQ=255)
                                                                                                                                gagaCCCCGGCATTCATGCGGGTGGGGACCTATGGCAGGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCTTCGGCAACACCTTCCACCTGTGGCTGCGTCCGGGCACCGAGGTGATCAAGAAGCACAAc               <  2:106184/147‑1 (MQ=255)
                                                                                                                                   ccaccGGCATGAATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCGGGCACCGAGGTGATCAAGAAGCACAACGGc            >  2:1010529/4‑150 (MQ=255)
                                                                                                                                   aCCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCGGGCACCGAGGTGATCAAGAAGCACAACGGc            >  2:161999/1‑150 (MQ=255)
                                                                                                                                     cccGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCAc                                                         >  1:307310/1‑103 (MQ=255)
                                                                                                                                     cccGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCAc                                                         <  2:307310/103‑1 (MQ=255)
                                                                                                                                     cccGGCATTCATGCCGGTGGGGAACTATGGCACGGTCAAGGGCATGCTACCACGCGACAGCGCGGCCATCGGCGCCGAGATGATGGTCAGCAACACCGTCCACCTGTGGCTGCGTCCGGGCAACGAGGTGATGCAGAAGCACAACGGCCt          >  2:457068/1‑150 (MQ=255)
                                                                                                                                             tCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCGGGCACCGAGGTGATCAAGAAGCACAACGGCCTGCACGAtt  <  1:344668/150‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                     
ACCCGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCGGGCACCGAGGTGATCAAGAAGCACAACGGCCTGCACGATT  >  NC_002947/970099‑970389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑