| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 972,118 | T→A | 8.3% | L136Q (CTG→CAG) | secD → | protein translocase subunit SecD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 972,118 | 0 | T | A | 8.3% | 171.7 / 7.1 | 60 | L136Q (CTG→CAG) | secD | protein translocase subunit SecD |
| Reads supporting (aligned to +/- strand): ref base T (21/34); new base A (3/2); total (24/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01 | |||||||||||
GCTGCCAGCCAAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGACGAT > NC_002947/971969‑972263 | gCTGCCAGCCAAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGCTGAAACTGGGCCTGGACCTATCCGGTGGTGTGCACTTCct > 1:186935/1‑150 (MQ=255) cagccaAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTgg < 2:448455/150‑1 (MQ=255) gccaAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGaa < 1:855316/150‑1 (MQ=255) aaGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtg < 2:927615/150‑1 (MQ=255) aaGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtg < 1:189892/150‑1 (MQ=255) aGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtgg < 2:518792/150‑1 (MQ=255) gATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggac < 1:1105674/150‑1 (MQ=255) gATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtgcac < 2:933241/150‑1 (MQ=255) aTGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggaca < 1:957220/150‑1 (MQ=255) gTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggacatg < 1:721802/150‑1 (MQ=255) aGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggacatgga < 2:1040675/150‑1 (MQ=255) cAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGc < 2:906106/150‑1 (MQ=255) ggCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCAt < 2:998715/150‑1 (MQ=255) tGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGACCAAACTGGGCCAAGACCTGGCGGGTGCTGTGCACTTACTGCTATACGTTGACATGGTTAAGGCCATGACTg > 1:327055/1‑150 (MQ=255) ttACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGaa > 1:762701/1‑150 (MQ=255) tACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGaaa < 2:59962/150‑1 (MQ=255) gtcgtGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtgga > 2:714655/1‑116 (MQ=255) gtcgtGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtgga < 1:714655/116‑1 (MQ=255) tcgtGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCt < 2:686984/150‑1 (MQ=255) cccTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGGTAGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAg < 1:633389/150‑1 (MQ=255) cTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCATGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGACCATGACTGCCCGCATGAAAGTCTACGAAGGc > 2:349043/1‑150 (MQ=255) tGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCg > 1:479152/1‑150 (MQ=255) aaCCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGAAAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAg < 1:1001196/150‑1 (MQ=255) cTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCAGCAGGAAGtgc > 2:58657/1‑99 (MQ=255) cTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCAGCAGGAAGtgc < 1:58657/100‑2 (MQ=255) gCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggacatggac > 1:752834/1‑105 (MQ=255) gCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggacatggac < 2:752834/105‑1 (MQ=255) gCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAg > 1:732874/1‑150 (MQ=255) ccAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAg < 2:12845/92‑1 (MQ=255) ccAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAg > 1:12845/1‑92 (MQ=255) gACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCAt > 1:1000402/1‑108 (MQ=255) gACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCAt < 2:1000402/108‑1 (MQ=255) tACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggacat > 2:351571/1‑93 (MQ=255) tACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggacat < 1:351571/93‑1 (MQ=255) ccccGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTtgctgc > 1:1747/1‑150 (MQ=255) cccGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTgcg > 2:653145/1‑150 (MQ=255) cGCAATGGCTGCGCAAGCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGt < 2:398204/133‑1 (MQ=255) cGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGt > 1:398204/1‑133 (MQ=255) cTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAgcgc < 2:376124/150‑1 (MQ=255) gcAACCTGGGCGCAAGCCCGATGAAACTGAGCCTCGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAACGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTcc < 1:43937/150‑1 (MQ=255) cTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGa < 2:1091409/113‑1 (MQ=255) cTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGa > 1:1091409/1‑113 (MQ=255) gcAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTTCTGGAAGTGGACATGGACAAAGACATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAgc < 1:80174/150‑1 (MQ=255) cGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTc > 1:429451/1‑150 (MQ=255) aaCTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAgg > 2:407082/1‑150 (MQ=255) aCTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGa > 1:493297/1‑101 (MQ=255) aCTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGa < 2:493297/101‑1 (MQ=255) aCTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGa > 1:4545/1‑150 (MQ=255) gggCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATgg < 1:407082/150‑1 (MQ=255) tGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGACGGCGGCATCATGc > 2:18149/1‑150 (MQ=255) tgtgCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGc < 1:108246/150‑1 (MQ=255) tgCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCaaa > 1:988750/1‑89 (MQ=255) tgCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCaaa < 2:988750/89‑1 (MQ=255) tgCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTg > 1:363502/1‑150 (MQ=255) tccACTTCCAGCAGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTg > 2:670091/3‑150 (MQ=255) ccACTTCCAGCAGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGa > 2:485465/2‑150 (MQ=255) cACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGTCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCCGCATCATGCTGGGCTTCGCTGAc < 2:1747/150‑1 (MQ=255) cACTTCCAGCAGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGAc < 1:485465/150‑1 (MQ=255) cACTTACTGCTGTGAGTGGAACTGGACAAGGCCATGACTGCCCGCATCAAAGTCTACGACGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGAATGGCGGCATCATGCTGGGCTTCGCTgtc < 2:761946/150‑3 (MQ=255) ttCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGACGAt < 2:762701/150‑1 (MQ=255) ttCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGACGGCGGCATCATGCTGGGCTTCGCTGACGAt < 1:18149/150‑1 (MQ=255) | GCTGCCAGCCAAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGACGAT > NC_002947/971969‑972263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |