Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,004,529 A→T 10.5% Q184L (CAG→CTG)  PP_0866 → transmembrane sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,004,5290AT10.5% 170.8 / 9.9 57Q184L (CAG→CTG) PP_0866transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base A (24/27);  new base T (4/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01

ATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCATATAGA  >  NC_002947/1004385‑1004676
                                                                                                                                                |                                                                                                                                                   
aTGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCa                                                                                                                                                >  1:596663/1‑150 (MQ=255)
aTGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCa                                                                                                                                                >  1:1201905/1‑150 (MQ=255)
  ggCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAgt                                                                                                                                              <  2:1206011/150‑1 (MQ=255)
  ggCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAgt                                                                                                                                              <  2:552533/150‑1 (MQ=255)
     tCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCTGGGCAgtgt                                                                                                                                            >  2:425711/1‑149 (MQ=255)
     tCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCTGGGCAgtgt                                                                                                                                            <  1:425711/149‑1 (MQ=255)
     tCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGtgtg                                                                                                                                           >  2:1145839/1‑150 (MQ=255)
     tCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGtgtg                                                                                                                                           >  1:177390/1‑150 (MQ=255)
      cGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGa                                                                                                                                          >  1:164217/1‑150 (MQ=255)
       gCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGAt                                                                                                                                         <  1:753593/150‑1 (MQ=255)
        cATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATc                                                                                                                                        <  1:552879/150‑1 (MQ=255)
             ggAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTcac                                                                                                                                   <  1:1034516/150‑1 (MQ=255)
              gAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTcaca                                                                                                                                  <  2:123490/150‑1 (MQ=255)
                gCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAgg                                                                                                                                >  2:858301/1‑150 (MQ=255)
                     aTGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTAccc                                                                                                                           <  1:1145839/150‑1 (MQ=255)
                        gCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCt                                                                                                                        >  2:975188/1‑150 (MQ=255)
                         cAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCtt                                                                                                                       <  2:582571/150‑1 (MQ=255)
                               gCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGAACATGAAAGAAAAGGAAGGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCAAAGGTACCCGCTTTGACGt                                                                                                                 >  1:574327/1‑150 (MQ=255)
                                        ggTCGAGTTCAGCGCCCGCCAGCGTCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCAACGTACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGa                                                                                                        <  1:380060/150‑1 (MQ=255)
                                          tCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGAcc                                                                                                      >  1:639897/1‑150 (MQ=255)
                                             aGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACccag                                                                                                   >  2:1045460/1‑150 (MQ=255)
                                              gTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACccagc                                                                                                  <  1:858301/150‑1 (MQ=255)
                                                       cGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCg                                                                                         >  2:1074593/1‑150 (MQ=255)
                                                       cGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGCTGCCTCTTTGACGTCCGCCTGGACCCAGCCAGCACCCg                                                                                         >  1:368241/1‑150 (MQ=255)
                                                          ccAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGATGTCCGCCTGGACCCAGCCAGCACCCgtgt                                                                                      <  2:596663/150‑1 (MQ=255)
                                                          ccAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGATGTCCGCCTGGACCCAGCCAGCACCCgtgt                                                                                      <  2:1201905/150‑1 (MQ=255)
                                                           cAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGtgtg                                                                                     <  1:251639/150‑1 (MQ=255)
                                                             gCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGc                                                                                   <  1:1045460/150‑1 (MQ=255)
                                                               ggCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCgg                                                                                 <  2:639897/150‑1 (MQ=255)
                                                                        gcCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAgg                                                                        <  1:1063484/150‑1 (MQ=255)
                                                                             ccttcTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCg                                                                   >  1:533849/5‑150 (MQ=255)
                                                                                   ggTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCgg                                                             <  1:1074593/150‑1 (MQ=255)
                                                                                          tGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCCGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAgg                                                      <  2:12770/150‑1 (MQ=255)
                                                                                          tGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAgg                                                      <  2:177390/150‑1 (MQ=255)
                                                                                          tGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAgg                                                      >  2:548349/1‑150 (MQ=255)
                                                                                             tGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCa                                                   <  1:975188/150‑1 (MQ=255)
                                                                                                   gCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCg                                             >  1:86292/1‑150 (MQ=255)
                                                                                                         cccATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGc                                       >  2:88601/1‑150 (MQ=255)
                                                                                                          ccATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCa                                                                                             >  1:836446/1‑95 (MQ=255)
                                                                                                          ccATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCa                                                                                             <  2:836446/95‑1 (MQ=255)
                                                                                                                 aGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCaa                               >  1:810006/1‑150 (MQ=255)
                                                                                                                         gCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGc                       >  2:324450/1‑150 (MQ=255)
                                                                                                                          ccGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGcc                      >  2:641881/1‑150 (MQ=255)
                                                                                                                           cGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCgg                                                             >  2:482369/1‑110 (MQ=255)
                                                                                                                           cGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCgg                                                             <  1:482369/110‑1 (MQ=255)
                                                                                                                             tttGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCggg                   <  1:641881/150‑1 (MQ=255)
                                                                                                                                   gatcacacTGCCCTGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAgggg             >  2:222212/5‑150 (MQ=255)
                                                                                                                                    atcacacTGCCCTGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAggg                                               >  2:643402/4‑115 (MQ=255)
                                                                                                                                    atcacacTGCCCTGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAggg                                               <  1:643402/112‑1 (MQ=255)
                                                                                                                                    atcacacTGCCCTGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGt            >  1:405728/4‑150 (MQ=255)
                                                                                                                                      gACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCgg                                                             >  2:660814/1‑99 (MQ=255)
                                                                                                                                      gACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCgg                                                             <  1:660814/99‑1 (MQ=255)
                                                                                                                                      gACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCg          >  2:708166/1‑150 (MQ=255)
                                                                                                                                        cacTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCa        <  1:324450/150‑1 (MQ=255)
                                                                                                                                        cacTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCa        <  2:810006/150‑1 (MQ=255)
                                                                                                                                        cacTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCa        >  1:896415/1‑150 (MQ=255)
                                                                                                                                              ccAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCATATAGa  <  1:708166/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCATATAGA  >  NC_002947/1004385‑1004676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑