| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,013,231 | A→T | 10.1% | intergenic (+56/+75) | prfC → / ← potF‑I | peptide chain release factor 3/putrescine‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,013,231 | 0 | A | T | 10.1% | 135.5 / 5.3 | 49 | intergenic (+56/+75) | prfC/potF‑I | peptide chain release factor 3/putrescine‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (23/20); new base T (3/2); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCG > NC_002947/1013083‑1013379 | cTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGaa < 1:497352/150‑1 (MQ=255) tGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAAt < 2:1180689/150‑1 (MQ=255) ctacctGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCAca > 1:1101928/1‑149 (MQ=255) ctGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGAc < 1:165235/150‑1 (MQ=255) gACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGt > 1:195451/1‑150 (MQ=255) gTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAGGTGa < 2:1139940/150‑1 (MQ=255) aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTT‑CGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcc > 1:282404/1‑150 (MQ=255) ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCt < 2:569204/150‑1 (MQ=255) tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAATTGCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGACCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgt < 1:162447/150‑1 (MQ=255) cTGATGGAAGAGCGCTGGCCGGACATCAAGTT‑CGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGc < 2:282404/150‑1 (MQ=255) tGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgt > 1:81722/1‑144 (MQ=21) tGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgt < 2:81722/144‑1 (MQ=21) gATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGa > 2:183564/1‑126 (MQ=255) gATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGa < 1:183564/126‑1 (MQ=255) gttGGCCGGACATCAAGTTTCGCGCTACCCGCGAGCACAGTTAAGGACTGAAATCGGGGCTGCGTTGCAGCCCTCCTCGGGCACGCCCACCCCCTCAGGAATCCCCCAGCCTGCCGGTCATGTGATACCTGTGGGAGCCGGCGTGC‑Ccgc < 2:365955/148‑1 (MQ=255) tGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGt > 1:222769/1‑142 (MQ=255) tGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGt < 2:222769/142‑1 (MQ=255) aGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCAc > 2:833666/1‑90 (MQ=255) aGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCAc < 1:833666/90‑1 (MQ=255) gTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAg > 2:1127586/1‑100 (MQ=255) gTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAg < 1:1127586/100‑1 (MQ=255) ttCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAggc‑gg > 2:83790/1‑150 (MQ=255) ccGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCCCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCAGGCGTGC‑CCGCGAAGAGCCGCTAGGC‑GGCCCCATTTCaa > 1:478411/1‑150 (MQ=255) aCCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATa < 2:515389/91‑1 (MQ=255) aCCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATa > 1:515389/1‑91 (MQ=255) ttAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAg < 2:191832/114‑1 (MQ=255) ttAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAg > 1:191832/1‑114 (MQ=255) aaGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGAc > 2:92716/1‑68 (MQ=255) aaGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGAc < 1:92716/68‑1 (MQ=255) gCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑GCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACt > 1:684666/1‑150 (MQ=255) ccTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTg > 2:589993/1‑150 (MQ=255) ccTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCATCACGCCGGCATCACCAGTCCTGGCGGTGATGTGATACCTGTGGGGGCTGGGGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTg < 2:739425/150‑1 (MQ=255) tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGa < 1:181926/67‑1 (MQ=255) tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGa > 2:181926/1‑67 (MQ=255) ggggggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCAGCAGACCTGGAGGTGATGGGATACCTGTGGGTGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGa < 2:478411/148‑1 (MQ=255) ttCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACCGTCg > 2:575096/1‑150 (MQ=255) aCGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGcc > 2:248032/1‑150 (MQ=255) gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCCCGTAAGAATACGGTAGATCTTCTCCgg > 1:525837/1‑150 (MQ=255) gCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATc < 1:589993/150‑1 (MQ=255) tCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTg < 1:879295/106‑1 (MQ=11) tCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTg > 2:879295/1‑106 (MQ=11) acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTc > 1:870188/1‑105 (MQ=255) acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTc < 2:870188/105‑1 (MQ=255) acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTAc < 1:330872/118‑1 (MQ=255) acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTAc > 2:330872/1‑118 (MQ=255) acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGAGCAGGTCGCGGTCCACCACG‑TGGGAGGCTTGGC‑CGGCCCATTTCAATTTCCGGCTTTGATACGGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTcc > 1:328108/1‑150 (MQ=255) caGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCa > 2:1044976/1‑150 (MQ=255) gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCtt < 2:1180301/90‑1 (MQ=255) gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCtt > 1:1180301/1‑90 (MQ=255) gAATCACCAGACCTGGAGGTCATGTGATAACTGTGGGGGGGGGCGTGC‑CTTCGAAGTTGCACCAGGCGGGCGCCATTCTACGTTTCGGGTAT‑TGAGTGGTCCTGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcg > 1:274919/1‑150 (MQ=255) | CTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCG > NC_002947/1013083‑1013379 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |