Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,013,251 A→T 9.8% intergenic (+76/+55) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,013,2510AT 9.8% 109.7 / 4.7 41intergenic (+76/+55)prfC/potF‑Ipeptide chain release factor 3/putrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (18/19);  new base T (2/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

GTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCC  >  NC_002947/1013107‑1013393
                                                                                                                                                |                                                                                                                                                
gTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAGGTGa                                                                                                                                             <  2:1139940/150‑1 (MQ=255)
   aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTT‑CGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcc                                                                                                                                         >  1:282404/1‑150 (MQ=255)
     ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCt                                                                                                                                        <  2:569204/150‑1 (MQ=255)
       tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAATTGCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGACCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgt                                                                                                                                      <  1:162447/150‑1 (MQ=255)
            cTGATGGAAGAGCGCTGGCCGGACATCAAGTT‑CGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGc                                                                                                                                <  2:282404/150‑1 (MQ=255)
             tGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgt                                                                                                                                      <  2:81722/144‑1 (MQ=21)
             tGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgt                                                                                                                                      >  1:81722/1‑144 (MQ=21)
                         gttGGCCGGACATCAAGTTTCGCGCTACCCGCGAGCACAGTTAAGGACTGAAATCGGGGCTGCGTTGCAGCCCTCCTCGGGCACGCCCACCCCCTCAGGAATCCCCCAGCCTGCCGGTCATGTGATACCTGTGGGAGCCGGCGTGC‑Ccgc                                                                                                                   <  2:365955/148‑1 (MQ=255)
                           tGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGt                                                                                                                          <  2:222769/142‑1 (MQ=255)
                           tGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGt                                                                                                                          >  1:222769/1‑142 (MQ=255)
                                          ttCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAggc‑gg                                                                                                 >  2:83790/1‑150 (MQ=255)
                                                     ccGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCCCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCAGGCGTGC‑CCGCGAAGAGCCGCTAGGC‑GGCCCCATTTCaa                                                                                      >  1:478411/1‑150 (MQ=255)
                                                             aCCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATa                                                                                                                                           >  1:515389/1‑91 (MQ=255)
                                                             aCCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATa                                                                                                                                           <  2:515389/91‑1 (MQ=255)
                                                                 ttAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAg                                                                                                               <  2:191832/114‑1 (MQ=255)
                                                                 ttAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAg                                                                                                               >  1:191832/1‑114 (MQ=255)
                                                                      gCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑GCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACt                                                                     >  1:684666/1‑150 (MQ=255)
                                                                       ccTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTg                                                                    >  2:589993/1‑150 (MQ=255)
                                                                       ccTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCATCACGCCGGCATCACCAGTCCTGGCGGTGATGTGATACCTGTGGGGGCTGGGGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTg                                                                    <  2:739425/150‑1 (MQ=255)
                                                                              ggggggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCAGCAGACCTGGAGGTGATGGGATACCTGTGGGTGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGa                                                             <  2:478411/148‑1 (MQ=255)
                                                                                                  ttCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACCGTCg                                         >  2:575096/1‑150 (MQ=255)
                                                                                                           aCGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGcc                                >  2:248032/1‑150 (MQ=255)
                                                                                                             gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCCCGTAAGAATACGGTAGATCTTCTCCgg                              >  1:525837/1‑150 (MQ=255)
                                                                                                                 gCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATc                          <  1:589993/150‑1 (MQ=255)
                                                                                                                   tCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTg                                                                    >  2:879295/1‑106 (MQ=11)
                                                                                                                   tCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTg                                                                    <  1:879295/106‑1 (MQ=11)
                                                                                                                       acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTc                                                                 >  1:870188/1‑105 (MQ=255)
                                                                                                                       acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTc                                                                 <  2:870188/105‑1 (MQ=255)
                                                                                                                       acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTAc                                                    >  2:330872/1‑118 (MQ=255)
                                                                                                                       acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTAc                                                    <  1:330872/118‑1 (MQ=255)
                                                                                                                       acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGAGCAGGTCGCGGTCCACCACG‑TGGGAGGCTTGGC‑CGGCCCATTTCAATTTCCGGCTTTGATACGGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTcc                    >  1:328108/1‑150 (MQ=255)
                                                                                                                        caGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCa                   >  2:1044976/1‑150 (MQ=255)
                                                                                                                           gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCtt                                                                            <  2:1180301/90‑1 (MQ=255)
                                                                                                                           gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCtt                                                                            >  1:1180301/1‑90 (MQ=255)
                                                                                                                           gAATCACCAGACCTGGAGGTCATGTGATAACTGTGGGGGGGGGCGTGC‑CTTCGAAGTTGCACCAGGCGGGCGCCATTCTACGTTTCGGGTAT‑TGAGTGGTCCTGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcg                >  1:274919/1‑150 (MQ=255)
                                                                                                                                aCCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc           <  1:83790/150‑1 (MQ=255)
                                                                                                                                  cAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAg         <  2:274919/150‑1 (MQ=255)
                                                                                                                                   aGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAAGTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGc        >  1:989640/1‑150 (MQ=255)
                                                                                                                                     aCCTGGAGGTCACGTGATGCCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGAT‑CAATCCTCGTTGCGCTCCGGCTTTGATACTGTGCCAGATTCGCGTACGTATACGGACGATGTTCGCCGGCATCGCCTCCAGCGCATACAGCtt      <  1:575096/150‑1 (MQ=255)
                                                                                                                                      ccTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTg     <  1:456989/150‑1 (MQ=255)
                                                                                                                                         ggAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGccc  <  1:248032/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGC‑CCGCGAAGGGCCGCTAGGC‑GGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCC  >  NC_002947/1013107‑1013393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑