| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,039,357 | G→C | 8.5% | A14G (GCC→GGC) | PP_0897 ← | class 1 fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,039,357 | 0 | G | C | 8.5% | 179.5 / 7.4 | 59 | A14G (GCC→GGC) | PP_0897 | class 1 fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (26/28); new base C (2/3); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
GACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGAAGCGCCTTGCAAGGCGCCGGCAGGTCAGGCCGACGGAAAAAG > NC_002947/1039208‑1039506 | gACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCgg > 2:1135406/1‑150 (MQ=255) gATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGa > 2:555863/1‑150 (MQ=255) cGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGctct < 1:813479/150‑1 (MQ=255) gcACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGAGCGTCGCCGACGCCCTgcaattc < 2:1140728/150‑7 (MQ=255) gcACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGAGCGTCGCCGACGCCCTgcaattc > 2:251227/1‑144 (MQ=255) atctgTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTATTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTctt < 1:30549/146‑1 (MQ=255) gAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTcttgc > 2:1203022/1‑150 (MQ=255) tCAGGATCTGGGCGATGGAATCGCGTGCAGCAGTCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCa > 1:515448/1‑150 (MQ=255) ggATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACgg > 2:1166643/1‑150 (MQ=255) ggATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACgg < 2:606296/150‑1 (MQ=255) ggATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACgg > 2:349922/1‑150 (MQ=255) tGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATg < 1:1135406/150‑1 (MQ=255) tGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATg < 1:555863/150‑1 (MQ=255) ggAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGAGCGTCGCCGAc < 1:592280/105‑1 (MQ=255) ggAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGAGCGTCGCCGAc > 2:592280/1‑105 (MQ=255) aaTCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGctc > 2:442546/1‑150 (MQ=255) aaTCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGctc < 2:411960/150‑1 (MQ=255) tCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGa < 1:276017/108‑1 (MQ=255) tCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGa > 2:276017/1‑108 (MQ=255) tCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGctcct > 2:9884/1‑150 (MQ=255) gcagGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCGTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTACTCTTAAAGAc > 2:481881/1‑150 (MQ=255) cagGGGAGTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAGTCGACGGGGTTGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACg < 2:522247/150‑1 (MQ=255) agGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACgg < 1:841253/150‑1 (MQ=255) agGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACgg > 2:847260/1‑150 (MQ=255) gATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAAc > 1:45504/1‑150 (MQ=255) aTTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACa > 1:723206/1‑150 (MQ=255) tCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACAtt < 2:723206/150‑1 (MQ=255) ccTCACGCAGATAGGCCTCGTGCATGGCCTGGCTGACACAGTCGGACTACTATTACGATATTAATTGCAGGGTGTAGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTc < 1:570584/150‑1 (MQ=255) tCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTcttgct > 1:607030/1‑103 (MQ=255) tCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTcttgct < 2:607030/103‑1 (MQ=255) tCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCaa > 1:89088/1‑150 (MQ=255) cGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCaaaaa < 1:1166643/150‑1 (MQ=255) cAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGa < 2:515448/150‑1 (MQ=255) gATAGGCCGCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACg < 1:596195/150‑1 (MQ=255) gCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCtt < 2:299437/120‑1 (MQ=255) gCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCtt > 1:299437/1‑120 (MQ=255) ccTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGAc > 1:71820/1‑150 (MQ=255) gTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAg < 1:442546/150‑1 (MQ=255) gTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAg < 1:349922/150‑1 (MQ=255) ggCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGAcc < 1:9884/150‑1 (MQ=255) ccTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAg > 1:658116/1‑150 (MQ=255) cTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTACTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGc < 1:481881/150‑1 (MQ=255) cTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGAGCGTCGCCGACGccct < 1:1182121/59‑1 (MQ=37) aTGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGt > 1:1006644/1‑150 (MQ=255) aaTCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGaa < 1:1203022/150‑1 (MQ=255) aTCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGTCGACTAGCGCGTCGAAg < 2:45504/150‑1 (MQ=255) aTCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGAAg > 2:98882/1‑150 (MQ=255) aTCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGAAg > 2:747494/1‑150 (MQ=255) gACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACg < 2:1017539/119‑1 (MQ=255) gACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACg > 1:1017539/1‑119 (MQ=255) ggtAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGAAGCGCCTTGCAAg < 2:658116/150‑1 (MQ=255) gtagtaCGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGAAGCGCCTTGCAAgg < 1:847260/150‑1 (MQ=255) taCGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGAAGCGCCTTGCAAGGCGcc > 1:57735/1‑150 (MQ=255) taCGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGAAGCGCCTTGCAAGGCGcc > 1:204976/1‑150 (MQ=255) gagaTGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAgcg > 1:741157/1‑68 (MQ=255) gagaTGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAgcg < 2:741157/68‑1 (MQ=255) agaTGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGAAgcgc > 2:611113/1‑133 (MQ=255) agaTGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGAAgcgc < 1:611113/133‑1 (MQ=255) agaTGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGAAGCGCCTTGCAAGGCGCCGGCa < 2:204976/150‑1 (MQ=255) tGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGAAGCGCCTTGCAAGGCGCCGGCAGGt > 2:882375/1‑150 (MQ=255) gCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGAAGCGCCTTGCAAGGCGCCGGCAGGTCAGGCCGACGGAAAAAg > 1:169357/1‑150 (MQ=255) | GACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGGGAACATTCAAAAAGACGCTCGACATAGCCGACCAGCACGTCGAAGCGCCTTGCAAGGCGCCGGCAGGTCAGGCCGACGGAAAAAG > NC_002947/1039208‑1039506 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |