| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,043,691 | A→C | 6.5% | D27A (GAC→GCC) | PP_0903 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,043,691 | 0 | A | C | 6.5% | 162.3 / 2.3 | 61 | D27A (GAC→GCC) | PP_0903 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (27/30); new base C (2/2); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
ACATTGGCATGACCTGATTCCAACGCTTGCATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGCCGCTGTTGGCGGAAAAGCCCAATCGGGTA > NC_002947/1043544‑1043839 | acaTTGGCATGACCTGATTCCAACGCTTGCATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACg < 1:1122099/150‑1 (MQ=255) aTTGGCATGACCTGATTCCAACGCTTGCATGACTAGTGTGGGCGCAATGGAGACAGGAGATGCCCAATGCGTAGCCCCATGAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAATCACCAGGACGGc > 1:678354/1‑150 (MQ=255) ttGGCATGACCTGATTCCAACGCTTGCATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGcc > 2:830268/1‑150 (MQ=255) gCATGACCTGATTCCAACGCTTGCATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTgg < 1:385519/150‑1 (MQ=255) gACCTGATTCCAACGCTTGCATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGCCGTCCTGgtgct < 1:363341/150‑2 (MQ=255) gACCTGATTCCAACGCTTGCATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGCCGTCCTGgtgct < 1:363262/150‑2 (MQ=255) tGATTCCAACGCTTGCATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGc > 1:102930/1‑150 (MQ=255) cAACGCTTGCATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTcc > 2:711251/1‑150 (MQ=255) cAACGCTTGCATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTcc > 2:367040/1‑150 (MQ=255) ttacATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAg > 1:1206059/4‑150 (MQ=255) gCATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCg > 2:1090603/1‑150 (MQ=255) cATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCga < 2:573180/150‑1 (MQ=255) cATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCga < 1:912169/150‑1 (MQ=255) aTGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCgag < 2:1206059/150‑1 (MQ=255) aTGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCgag < 2:910570/150‑1 (MQ=255) aTGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCgag > 1:738423/1‑150 (MQ=255) gtgtGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGa < 2:497045/124‑1 (MQ=255) gtgtGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGa > 1:497045/1‑124 (MQ=255) cAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCtt < 1:1059861/150‑1 (MQ=255) ggAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCgcggcgcgccaact < 1:1014964/150‑5 (MQ=255) gaTGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACACCGAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCAt < 1:676151/150‑1 (MQ=255) cccAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTggc > 2:685828/1‑150 (MQ=255) ccAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGCCCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGAAGCCTGTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTggcg > 1:564871/1‑150 (MQ=255) cAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGGCCGGTTGGCGCGCCGCGCCTTGGCATTggccg > 1:907539/1‑148 (MQ=255) aTGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTggcgggc < 1:685828/150‑1 (MQ=255) gCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGa < 1:711251/150‑1 (MQ=255) gTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCgcggcgcgccaa > 2:292002/1‑131 (MQ=255) gTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCgcggcgcgccaa < 1:292002/133‑3 (MQ=255) aGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAgcct < 2:566654/150‑1 (MQ=255) ccccATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAgcctgg > 2:1033418/1‑150 (MQ=255) cccATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAgcctggc > 1:89427/1‑150 (MQ=255) gCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAg < 1:601494/103‑1 (MQ=255) gCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAg > 2:601494/1‑103 (MQ=255) gAATTTTCCGAGAAGTCCGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGAc < 1:367040/150‑1 (MQ=255) ttttCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCtt < 1:752124/113‑1 (MQ=255) ttttCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCtt > 2:752124/1‑113 (MQ=255) gagaAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGc > 2:1115595/1‑150 (MQ=255) aCGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCgg < 1:960047/150‑1 (MQ=255) gACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGcc < 1:816700/46‑1 (MQ=255) gACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGcc > 2:816700/1‑46 (MQ=255) aCAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGcc > 1:914435/1‑150 (MQ=255) aaaGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGGCTACGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGt > 1:616793/1‑150 (MQ=255) aaaGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGt < 2:102930/150‑1 (MQ=255) ccATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTg < 1:1115595/150‑1 (MQ=255) aTGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGc < 2:914435/150‑1 (MQ=255) gcgAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGCCGCTGt < 1:1090603/150‑1 (MQ=255) gAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGCCGCTGTTg > 1:226792/1‑150 (MQ=255) cttccgatctcAAGCACCAGGACGGCCT‑GCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGTGCAGCGGGCCGTTTCTGGCCGCTGTTGGc < 1:939140/143‑1 (MQ=255) tACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGCCGCTGTTGGCg < 2:226792/150‑1 (MQ=255) tttCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCg < 1:680747/118‑1 (MQ=255) tttCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCg > 2:680747/1‑118 (MQ=255) tttCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGCCGCTGTTGGCGGaa > 2:73525/1‑150 (MQ=255) gccgcgccAGGCCGTCCTGGTGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGCCGCTGTTGGCGGAAAAGcc > 1:1136944/7‑150 (MQ=255) tcgcgccAGGCCGTCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTCCTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGCCGCTGTTGGCGGAAAAGccc > 1:128264/6‑150 (MQ=255) aaGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGttt > 1:1185896/1‑125 (MQ=255) aaGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGttt < 2:1185896/125‑1 (MQ=255) aaGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGCCGCTGTTGGCGGAAAAGccc < 1:145370/150‑1 (MQ=255) aCCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGCCGCTGTTGGCGGAAAAGCCCAATc > 2:725786/1‑150 (MQ=255) cAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGCCGCTGTTGGCGGAAAAGCCCAATCgg < 2:593257/150‑1 (MQ=255) cAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGCCGCTGTTGGCGGAAAAGCCCAATCgg > 1:593257/1‑150 (MQ=255) aGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGCCGCTGTTGGCGGAAAAGCCCAATCggg < 2:89427/150‑1 (MQ=255) aaCGGCCTGGCGCGACGACTTTCCCACAAGCGAGATGAGCAGTTGGCGCGGCGCGCCTTGGCATTGGCGGGCGAGGCTGGCCTGGTACTCGACCTGCCATGCGGAGAGGGCCGTTTCTGGCCGCTGTTGGCGGAACAGCCCAATCGGGTa < 2:678354/149‑1 (MQ=255) | ACATTGGCATGACCTGATTCCAACGCTTGCATGACTAGTGTGGGCGCAATGGAGTCAGGAGATGCCCAATGCGTAGCCCCATCAAGCTTGAATTTTCCGAGAAGTACGACAAAGACCATGCGCGCGAGTACTTTCTCAAGCACCAGGACGGCCTGGCGCGACGCCTTTCCCACAAGCGAGATGAGCAGTTGGCGCGCCGCGCCTTGGCATTGGCGGGCGAGCCTGGCCTGGTACTCGACCTGCCATGCGGAGCGGGCCGTTTCTGGCCGCTGTTGGCGGAAAAGCCCAATCGGGTA > NC_002947/1043544‑1043839 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |