Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,068,338 C→T 9.7% S167S (AGC→AGT)  PP_0926 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,068,3380CT 9.7% 112.0 / 3.1 43S167S (AGC→AGT) PP_0926membrane protein
Reads supporting (aligned to +/- strand):  ref base C (20/18);  new base T (2/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GCCGCCAGCCTGTACGGTGACTATGGCATTTCCCTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCT  >  NC_002947/1068192‑1068487
                                                                                                                                                     |                                                                                                                                                     
gccgccAGCCTGTACGGTGACTATGGCATTTCCCTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCAtt                                                                                                                                                    <  2:513556/150‑1 (MQ=255)
    ccAGCCTGTACGGTGACTATGGCATTTCCCTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCa                                                                                                                                                <  1:51540/150‑1 (MQ=255)
                     tagggCATTTCCCTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGc                                                                                                                                                       >  2:621430/4‑126 (MQ=255)
                     tATGGCATTTCCCTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGc                                                                                                                                                       <  1:621430/126‑1 (MQ=255)
                     tATGGCATTTCCCTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGatcatc                                                                                                                               >  2:70726/1‑150 (MQ=255)
                       tGGCATTTCCCTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGTATTTTCAGCAACCCGCTGATCATCAg                                                                                                                             >  2:764/1‑150 (MQ=255)
                          cATTTCCCTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGt                                                                                                                          >  1:588616/1‑150 (MQ=255)
                           aTTTCCCTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGatc                                                                                                                                  <  2:36112/141‑1 (MQ=255)
                           aTTTCCCTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGatc                                                                                                                                  >  1:36112/1‑141 (MQ=255)
                                ccTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAGCTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCCGCGTGCtggt                                                                                                                    <  1:175619/150‑1 (MQ=255)
                                ccTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCtggt                                                                                                                    >  1:1030919/1‑150 (MQ=255)
                                ccTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCtggt                                                                                                                    <  1:656040/150‑1 (MQ=255)
                                                gCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGTATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCt                                                                                                    >  2:757624/1‑150 (MQ=255)
                                                    gCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACg                                                                                                <  1:1150309/150‑1 (MQ=255)
                                                      cTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGTCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGAGCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGc                                                                                              >  1:389459/1‑150 (MQ=255)
                                                      cTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACg                                                                                                <  1:573972/148‑1 (MQ=255)
                                                      cTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACg                                                                                                >  2:573972/1‑148 (MQ=255)
                                                           cATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAG‑CCAGACGTGTGCTCTTCCGATC‑‑TGCCGCAGCTGCAAGAGGAGGTTCAACCACCCGCTGCTCTACCTCGTTCTCGTGGGCGCGCCCATGGCTTACGGCCAGGt                                                                                         <  1:1178421/150‑1 (MQ=255)
                                                                tCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGc                                                                                              <  1:1054012/140‑1 (MQ=255)
                                                                tCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGc                                                                                              >  2:1054012/1‑140 (MQ=255)
                                                                tCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGc                                                                                    >  2:485443/1‑150 (MQ=255)
                                                                        aaCTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGc                                                                                                                                       >  1:1040185/1‑91 (MQ=255)
                                                                        aaCTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGc                                                                                                                                       <  2:1040185/91‑1 (MQ=255)
                                                                          cTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGCCCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTg                                                                          >  1:761228/1‑150 (MQ=255)
                                                                           tCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTgg                                                                         >  1:910404/1‑150 (MQ=255)
                                                                               tGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTAc                                                                     <  2:1030919/150‑1 (MQ=255)
                                                                                gTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGTATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACt                                                                    <  1:764/150‑1 (MQ=255)
                                                                                gTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACt                                                                    <  2:588616/150‑1 (MQ=255)
                                                                                              tGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACt                                                      >  1:1200649/1‑150 (MQ=255)
                                                                                                       aCAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGcac                                             <  1:70726/150‑1 (MQ=255)
                                                                                                          gCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGa                                          >  2:492755/1‑150 (MQ=255)
                                                                                                           cccGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTg                                                                                                                         >  2:766201/1‑70 (MQ=255)
                                                                                                           cccGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTg                                                                                                                         <  1:766201/70‑1 (MQ=255)
                                                                                                           cccGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGAt                                         <  2:389459/150‑1 (MQ=255)
                                                                                                                  cT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTAGCACAGATGACCCTg                                  >  2:735477/1‑150 (MQ=255)
                                                                                                                   t‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGc                                 >  1:596733/1‑150 (MQ=255)
                                                                                                                       cAAGTCCGATCCGTGGAGCATCTGCAAGAGTATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGcc                                <  1:757624/150‑1 (MQ=255)
                                                                                                                          gTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCt                             <  1:1162913/150‑1 (MQ=255)
                                                                                                                              gATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCggg                                                           >  1:181938/1‑116 (MQ=255)
                                                                                                                              gATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCggg                                                           <  2:181938/116‑1 (MQ=255)
                                                                                                                                   gTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGccc                                                                                                          <  2:2202/64‑1 (MQ=255)
                                                                                                                                   gTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGccc                                                                                                          >  1:2202/1‑64 (MQ=255)
                                                                                                                                         cATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGcaca                                            <  1:64575/120‑1 (MQ=255)
                                                                                                                                         cATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGcaca                                            >  2:64575/1‑120 (MQ=255)
                                                                                                                                              gCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATcggcg         >  1:881072/1‑150 (MQ=255)
                                                                                                                                                     cATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCt  <  1:485443/150‑1 (MQ=255)
                                                                                                                                                     cATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCt  >  1:874340/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCGCCAGCCTGTACGGTGACTATGGCATTTCCCTGGGGGCGGTACTCGCAGGCCTGGTCATCCTCATGTACAACTCGCTGTCAGCGGTGGTACTGGCGGTGTACAGCCCGGACCT‑‑‑CAAGTCCGATCCGTGGAGCATCTGCAAGAGCATTTTCAGCAACCCGCTGATCATCAGCGTGCTGGTGGCCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCT  >  NC_002947/1068192‑1068487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑