Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,084,916 C→T 7.3% L246L (CTG→TTG)  PP_0939 → carbon‑nitrogen hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,084,9160CT 7.3% 153.2 / 2.4 55L246L (CTG→TTG) PP_0939carbon‑nitrogen hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base T (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

GCGTGCCCGGGCCATCGAAACCCAGTGTTACCTGCTGGCTGCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTCGGAGTGACTATGAGCCAGATGTTATCCACCGTCAGTGAGC  >  NC_002947/1084768‑1085065
                                                                                                                                                    |                                                                                                                                                     
tcgtgcCCGGGCCATCGAAGCGCAGTGTTACCTGCTGGCTGCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACt                                                                                                                                                      <  2:258085/149‑1 (MQ=255)
        gggCCATCGAAACCCAGTGTTACCTGCTGGCTGCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCg                                                                                                                                              >  2:713253/1‑150 (MQ=255)
             aTCGAAACCCAGTGTTACCTGCTGGCTGCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGAGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAgcgc                                                                                                                                         <  1:84437/150‑1 (MQ=255)
                gAAACCCAGTGTTACCTGCTGGCTGCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGAc                                                                                                                                      <  2:359695/150‑1 (MQ=255)
                   aCCCAGTGTTACCTGCTGGCTGCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACAtt                                                                                                                                   <  1:713253/150‑1 (MQ=255)
                     ccAGTGTTACCTTCTGGCTGCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTgc                                                                                                                                 >  1:805334/1‑149 (MQ=255)
                      cAGTGTTACCTGCTGGCTGCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTgaa                                                                                                                                >  2:529951/1‑150 (MQ=255)
                       aGTGTTACCTTCTGGCTGCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTgaag                                                                                                                               <  2:805334/150‑1 (MQ=255)
                         tgtTACCTGCTGGCTGCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTgaagaa                                                                                                                             <  2:535119/150‑1 (MQ=255)
                           ttACCTGCTGGCTGCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACa                                                                                                                           >  2:54947/1‑150 (MQ=255)
                               ctgctgGCTGCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCg                                                                                                                       >  2:1173340/1‑150 (MQ=255)
                                 gctggctcCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTc                                                                                                                     >  2:141411/1‑150 (MQ=255)
                                  ctggctgCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTca                                                                                                                    >  1:1007959/1‑149 (MQ=255)
                                   tggctgCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACACATGGCCATGGGGCGATCGTCGACCCTTGGTGTCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCa                                                                                                                   >  1:750602/1‑150 (MQ=255)
                                     gctgCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACAtt                                                                                                                                   <  1:582259/132‑1 (MQ=255)
                                     gctgCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACAtt                                                                                                                                   >  2:582259/1‑132 (MQ=255)
                                      ctgCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTc                                                                                                                <  1:54947/150‑1 (MQ=255)
                                          ggCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTATTGCTTGCCGAGCGCGACAtt                                                                                                                                   <  2:194159/127‑1 (MQ=255)
                                          ggCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTATTGCTTGCCGAGCGCGACAtt                                                                                                                                   <  2:194081/127‑1 (MQ=255)
                                          ggCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTATTGCTTGCCGAGCGCGACAtt                                                                                                                                   >  1:194159/1‑127 (MQ=255)
                                          ggCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTATTGCTTGCCGAGCGCGACAtt                                                                                                                                   >  1:194081/1‑127 (MQ=255)
                                                 ggCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTcc                                                                                                                    <  1:8409/135‑1 (MQ=255)
                                                 ggCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTcc                                                                                                                    >  2:8409/1‑135 (MQ=255)
                                                         ccACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCggtggt                                                                                             >  1:168892/1‑150 (MQ=255)
                                                              cTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACGTTGAAGAACAAGCGTCCATTCGGACGCGCATGGCGGTGGTATCGc                                                                                        >  1:539035/1‑150 (MQ=255)
                                                                     aCGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGgcg                                                                                 >  1:737665/1‑150 (MQ=255)
                                                                          aaaCCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACCTTTTGGGCGGTGGTATCGCACCGGCGCtttt                                                                            <  1:31340/150‑1 (MQ=255)
                                                                               cATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCg                                                                       >  2:943095/1‑150 (MQ=255)
                                                                                aTGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACgcgc                                                                                                        <  1:813817/116‑1 (MQ=255)
                                                                                aTGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACgcgc                                                                                                        >  2:813817/1‑116 (MQ=255)
                                                                                  ggCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAg                                                                    <  2:394161/150‑1 (MQ=255)
                                                                                                    gACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGc                                                       <  1:545620/145‑1 (MQ=255)
                                                                                                    gACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGc                                                       >  2:545620/1‑145 (MQ=255)
                                                                                                    gACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTgcg                                                  >  1:1115215/1‑150 (MQ=255)
                                                                                                       ccTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCAt                                               >  2:578243/1‑150 (MQ=255)
                                                                                                         ttGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCgg                                                                                   >  2:145112/1‑112 (MQ=255)
                                                                                                         ttGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCgg                                                                                   <  1:145112/112‑1 (MQ=255)
                                                                                                         ttGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATAc                                             <  1:141411/150‑1 (MQ=255)
                                                                                                          tGGGGGCGCATTCTCGCAGAACAGGCGAACGGTGACACGGTACATCATCCCTATCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATAcc                                            <  1:1173340/150‑1 (MQ=255)
                                                                                                            ggggCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTc                                          >  2:929873/1‑150 (MQ=255)
                                                                                                            ggggCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTc                                          <  2:1115215/150‑1 (MQ=255)
                                                                                                             gggCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCAGCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTCg                                         <  2:168892/150‑1 (MQ=255)
                                                                                                              ggCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTCgg                                        >  2:1003125/1‑150 (MQ=255)
                                                                                                               gcgcATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTCGGa                                       <  2:28415/150‑1 (MQ=255)
                                                                                                                   aTTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCggtgg                                                                                              <  1:695075/91‑1 (MQ=255)
                                                                                                                   aTTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTCGGAGTGa                                   <  2:750602/150‑1 (MQ=255)
                                                                                                                   aTTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTCGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCggtgg                                                                                              >  2:695075/1‑91 (MQ=255)
                                                                                                                         gCAGAACAGGCGCAAGGTGAAGCTGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTCGGAGTGACTATGa                             <  1:408822/150‑1 (MQ=255)
                                                                                                                            gAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGc                                                                                                                        >  1:928390/1‑56 (MQ=255)
                                                                                                                            gAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGc                                                                                                                        <  2:928390/56‑1 (MQ=255)
                                                                                                                            gAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTCGGAGTGACTATGAGcc                          <  1:943095/150‑1 (MQ=255)
                                                                                                                               cAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTCGGAGTGACTATGAGCCAGa                       <  2:35384/150‑1 (MQ=255)
                                                                                                                                    gcAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCGTTCGGAAGCGCATGCCGGTGGGGTGGCACCTGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTCGGAGTGACTATGAGCCAGATGTTa                  >  1:146795/1‑150 (MQ=255)
                                                                                                                                      aaGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCAATTCGGACGCGCGTGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTCGGAGTGACTATGAGCCAGATGTTATc                <  2:1007959/150‑1 (MQ=255)
                                                                                                                                              gCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGCCGCCTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTCGGAGTGACTATGAGCCAGATGTTATCCACCGTCa        >  1:79084/1‑150 (MQ=255)
                                                                                                                                                    cTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTCGGTGTGACTATGAGCCAGATGTTATCCACCGTCAGTGagc  <  1:976920/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCGTGCCCGGGCCATCGAAACCCAGTGTTACCTGCTGGCTGCGGCACAGGGCGGTGCCCACCCTGGCCCACGGGAAACCCATGGCCATGCGGCGATCGTCGACCCTTGGGGGCGCATTCTCGCAGAACAGGCGCAAGGTGAAGCGGTACTGCTTGCCGAGCGCGACATTGAAGAACAAGCGTCCATTCGGACGCGCATGCCGGTGGTATCGCACCGGCGCTTTTTTTCGCAGGGCGCGTTGCGGCCTGCGCATACCTCGGAGTGACTATGAGCCAGATGTTATCCACCGTCAGTGAGC  >  NC_002947/1084768‑1085065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑