Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,121,458 C→A 7.7% R298R (CGC→CGA)  PP_0982 → YjgP/YjgQ family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,121,4580CA 7.7% 200.5 / 3.5 65R298R (CGC→CGA) PP_0982YjgP/YjgQ family permease
Reads supporting (aligned to +/- strand):  ref base C (32/28);  new base A (3/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

CGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCC  >  NC_002947/1121314‑1121591
                                                                                                                                                |                                                                                                                                     
cGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAggg                                                                                                                                  >  1:782121/1‑150 (MQ=255)
 gAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGc                                                                                                                                 <  2:602378/150‑1 (MQ=255)
       gAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTc                                                                                                                           >  2:407145/1‑150 (MQ=255)
            gcTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAACGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTTGACCCGCGACAgc                                                                                                                                   <  2:320426/137‑2 (MQ=255)
            gcTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAACGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTTGACCCGCGACAgc                                                                                                                                   >  1:320426/1‑136 (MQ=255)
             cTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAg                                                                                                                     >  1:441329/1‑150 (MQ=255)
                 ccACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctgc                                                                                                                 >  2:61951/1‑150 (MQ=255)
                  cACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctgct                                                                                                                <  1:106302/150‑1 (MQ=255)
                  cACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGATGATAAGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGGGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctgct                                                                                                                >  1:634412/1‑150 (MQ=255)
                   aCCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGctgctg                                                                                                               >  2:603273/1‑150 (MQ=255)
                      tCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCg                                                                                                            <  2:715602/150‑1 (MQ=255)
                            cTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTACTCAAGCTGCTGCAGGAAAtt                                                                                                      >  1:203382/1‑150 (MQ=255)
                             tGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTc                                                                                                                           >  2:449367/1‑128 (MQ=255)
                             tGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTc                                                                                                                           <  1:449367/128‑1 (MQ=255)
                               aTCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttctt                                                                                                   >  1:873676/1‑150 (MQ=255)
                                   gCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAg                                                                                                                                    >  1:271558/1‑113 (MQ=255)
                                   gCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAg                                                                                                                                    <  2:271558/113‑1 (MQ=255)
                                   gCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGt                                                                                               >  2:57584/1‑150 (MQ=255)
                                   gCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGt                                                                                               >  1:894277/1‑150 (MQ=255)
                                    cAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTa                                                                                              <  2:688160/150‑1 (MQ=255)
                                          gggCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGc                                                                                        <  1:746227/150‑1 (MQ=255)
                                              cTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTAc                                                                                    <  1:603273/150‑1 (MQ=255)
                                                gCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCt                                                                                  <  2:441329/150‑1 (MQ=255)
                                                      gcgcgcCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATtcttct                                                                                                 >  2:846000/1‑129 (MQ=255)
                                                      gcgcgcCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATtcttct                                                                                                 <  1:846000/129‑1 (MQ=255)
                                                       cgcgcCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATg                                                                           <  1:57584/150‑1 (MQ=255)
                                                        gcgcCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGc                                                                          <  2:873676/150‑1 (MQ=255)
                                                         cgcCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCCGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCt                                                                         <  2:646189/150‑1 (MQ=255)
                                                          gcCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCACGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTg                                                                        >  2:382646/1‑150 (MQ=255)
                                                           ccGAGCTGCAATGGCGGTTGTCGCTGGCGTTCCTGGTGTTCGTCATCACCCTGCTGGCGGTGCTGCTGTCGCGGGTCAAGCCCCGCCCGGGCCGCTTCCTCAAGCTGCCGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGGTgg                                                                       >  2:397982/1‑149 (MQ=255)
                                                            cGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGAt                                                                      <  1:61951/150‑1 (MQ=255)
                                                               gCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCTGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTc                                                                   <  2:360237/150‑1 (MQ=255)
                                                                cTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTcc                                                                  >  1:494329/1‑150 (MQ=255)
                                                                cTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGGCGCGGGTTGACCCGCGACAgc                                                                                                                                   >  1:1142372/1‑84 (MQ=38)
                                                                cTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGGCGCGGGTTGACCCGCGACAgc                                                                                                                                   <  2:1142372/85‑2 (MQ=38)
                                                                   cAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATtcttct                                                                                                 <  2:87370/116‑1 (MQ=255)
                                                                   cAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATtcttct                                                                                                 >  1:87370/1‑116 (MQ=255)
                                                                            ttGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGcc                                                      >  1:1042798/1‑150 (MQ=255)
                                                                               tCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTc                                                   >  1:120008/1‑150 (MQ=255)
                                                                                   tGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCgaga                                               >  1:809237/1‑150 (MQ=255)
                                                                                     ccGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCATCGAGaa                                              <  2:1190722/149‑1 (MQ=255)
                                                                                     ccGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCATCGAGaa                                              >  1:1190722/1‑149 (MQ=255)
                                                                                       gATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATgg                                                                                         >  2:161496/1‑104 (MQ=255)
                                                                                       gATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATgg                                                                                         <  1:161496/104‑1 (MQ=255)
                                                                                       gATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAggg                                           >  1:945216/1‑150 (MQ=255)
                                                                                             ggTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAgg                                            <  1:661409/143‑1 (MQ=255)
                                                                                             ggTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAgg                                            >  2:661409/1‑143 (MQ=255)
                                                                                               tgtTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGc                                   <  2:120008/150‑1 (MQ=255)
                                                                                                        tcACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGaagatcg                          >  1:1103354/1‑144 (MQ=255)
                                                                                                          aCCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTg                        >  1:823101/1‑150 (MQ=255)
                                                                                                           cgctgctgGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTgg                       >  2:621815/3‑150 (MQ=255)
                                                                                                           cccTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTgg                       <  2:782121/150‑1 (MQ=255)
                                                                                                            ccTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTggg                      >  1:962749/1‑150 (MQ=255)
                                                                                                                ctgGCGGTGCCGCTGTCGCGGGTCCACCAGCGCCGGGGGCGGCTCAAGAACCTGGGGGCGGCAATTCTTCTGTATAGAGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCAtg                  <  1:870940/150‑1 (MQ=255)
                                                                                                                    cGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATgtggt              <  2:494329/150‑1 (MQ=255)
                                                                                                                      gTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGtggtgg            <  2:823101/150‑1 (MQ=255)
                                                                                                                         gccctggcgcgGGTTGACCCGCGACAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTc         >  1:954627/8‑150 (MQ=255)
                                                                                                                           gCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCa       >  1:406086/1‑150 (MQ=255)
                                                                                                                                cgcgGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGcc  <  2:962749/150‑1 (MQ=255)
                                                                                                                                 gcgGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGa                                >  1:961049/1‑119 (MQ=255)
                                                                                                                                 gcgGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGa                                <  2:961049/119‑1 (MQ=255)
                                                                                                                                         aCCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTa                                                               >  2:616145/1‑80 (MQ=255)
                                                                                                                                         aCCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTa                                                               <  1:616145/80‑1 (MQ=255)
                                                                                                                                          cccGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGcc                                  <  2:762315/108‑1 (MQ=255)
                                                                                                                                          cccGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGcc                                  >  1:762315/1‑108 (MQ=255)
                                                                                                                                                |                                                                                                                                     
CGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCC  >  NC_002947/1121314‑1121591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑