Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,221,052 C→A 13.8% G87V (GGC→GTC)  dctD‑II ← C4‑dicarboxylate transport transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,221,0520CA13.8% 143.8 / 9.0 50G87V (GGC→GTC) dctD‑IIC4‑dicarboxylate transport transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (21/22);  new base A (3/4);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAGCGCCTGTTCG  >  NC_002947/1220905‑1221198
                                                                                                                                                   |                                                                                                                                                  
tGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCa                                                                                                                                                  <  2:267099/150‑1 (MQ=255)
 ggcggcGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCAt                                                                                                                                                 <  1:492359/150‑1 (MQ=255)
 ggcggcGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGGCGATATCGACAt                                                                                                                                                 <  1:911150/150‑1 (MQ=255)
    ggcgCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGc                                                                                                                                              >  1:1000895/1‑150 (MQ=255)
    ggcgCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGc                                                                                                                                              <  2:429991/150‑1 (MQ=255)
        cAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGt                                                                                                                                          <  1:685403/150‑1 (MQ=255)
         aGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGGCGATATCGACATGGccgttg                                                                                                                                         <  1:771745/150‑5 (MQ=255)
               accacTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGc                                                                                                                                   >  1:538991/1‑150 (MQ=255)
                         gTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCa                                                                                                                         >  1:107263/1‑150 (MQ=255)
                               gCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGc                                                                                                                   <  1:57729/150‑1 (MQ=255)
                                 ccGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCgg                                                                                                                 >  1:95251/1‑150 (MQ=255)
                                     gcggTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCAc                                                                                                             >  1:1205728/4‑150 (MQ=255)
                                       tGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATAGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACgg                                                                                                           <  1:580192/150‑1 (MQ=255)
                                                     gcgTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGAtt                                                                                             <  1:113128/150‑1 (MQ=255)
                                                       gTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGa                                                                                           <  2:1000895/150‑1 (MQ=255)
                                                        tACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGag                                                                                          <  2:95251/150‑1 (MQ=255)
                                                         aCCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGagc                                                                                         >  2:1026450/1‑150 (MQ=255)
                                                                 gACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAg                                                                                 <  1:1013554/150‑1 (MQ=255)
                                                                  aCCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAgg                                                                                >  2:1048778/1‑150 (MQ=255)
                                                                      ggCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCAt                                                                            <  1:464765/150‑1 (MQ=255)
                                                                      ggCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCAt                                                                            <  1:693173/150‑1 (MQ=255)
                                                                       gcgcTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATc                                                                           >  1:1029145/1‑150 (MQ=255)
                                                                           tCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCACTGCTGAGCTACAATCACTAGCGTCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATa                                                                       >  1:1177868/1‑150 (MQ=255)
                                                                               gggAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCg                                                                   <  1:1179637/150‑1 (MQ=255)
                                                                                ggAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATAccc                                                                    <  1:234567/148‑1 (MQ=255)
                                                                                ggAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATAccc                                                                    >  2:234567/1‑148 (MQ=255)
                                                                                   gaACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGCGCCTTGAGGCGATTGAGCAGATCCCGTCGATCAATACCCGGCAg                                                               <  2:107263/150‑1 (MQ=255)
                                                                                    aaCGGTTTTTCTTTTTAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAgg                                                              >  2:1066298/1‑150 (MQ=255)
                                                                                                aTGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTg                                                  >  1:310357/1‑150 (MQ=255)
                                                                                                            gcgcCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAgg                                                                                >  2:896537/1‑108 (MQ=255)
                                                                                                            gcgcCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAgg                                                                                <  1:896537/108‑1 (MQ=255)
                                                                                                              gcCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGc                                                                                                                   <  1:35248/71‑1 (MQ=255)
                                                                                                              gcCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGc                                                                                                                   >  2:35248/1‑71 (MQ=255)
                                                                                                              gcCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGc                                    >  1:646178/1‑150 (MQ=255)
                                                                                                                       cATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAAtcgtc                           <  2:1177868/150‑1 (MQ=255)
                                                                                                                        aTGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCg                                      >  1:1116773/1‑138 (MQ=255)
                                                                                                                        aTGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCg                                      <  2:1116773/138‑1 (MQ=255)
                                                                                                                        aTGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAAtcgtcg                          >  2:256430/1‑150 (MQ=255)
                                                                                                                         tGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGc                         <  1:315961/150‑1 (MQ=255)
                                                                                                                             gcCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGAt                     >  2:1083158/1‑150 (MQ=255)
                                                                                                                                  tggCCATGGCGATATCGACATGGCCGGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATAccc                                                                    <  2:1071212/97‑1 (MQ=39)
                                                                                                                                  tggCCATGGCGATATCGACATGGCCGGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATAccc                                                                    >  1:1071212/2‑98 (MQ=39)
                                                                                                                                  tggCCATGGCGATATCGACATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTga                                                 >  2:466168/2‑117 (MQ=255)
                                                                                                                                  tggCCATGGCGATATCGACATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTga                                                 <  1:466168/116‑1 (MQ=255)
                                                                                                                                  cGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGtt                <  2:310357/150‑1 (MQ=255)
                                                                                                                                        tggcGATATCGACATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACACGTCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAgcg          >  1:617491/4‑150 (MQ=255)
                                                                                                                                        tGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGAAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAgcg          >  1:986263/1‑150 (MQ=255)
                                                                                                                                           cGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAGCGCCt       >  2:879950/1‑150 (MQ=255)
                                                                                                                                              tatCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAGCGCCTGtt    <  2:1205728/150‑1 (MQ=255)
                                                                                                                                                tCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAGCGCCTGTTCg  >  1:887237/1‑150 (MQ=255)
                                                                                                                                                   ccATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAgcgc         >  2:714472/1‑140 (MQ=255)
                                                                                                                                                   ccATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAgcgc         <  1:714472/140‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAGCGCCTGTTCG  >  NC_002947/1220905‑1221198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑