| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,245,344 | G→C | 17.2% | R64G (CGC→GGC) | pyrC ← | dihydroorotase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,245,344 | 0 | G | C | 17.2% | 120.8 / 18.5 | 48 | R64G (CGC→GGC) | pyrC | dihydroorotase |
| Reads supporting (aligned to +/- strand): ref base G (21/18); new base C (5/3); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GCCGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCACCATCGCGCAGATGGATGT > NC_002947/1245195‑1245493 | gCCGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCcgggcg > 2:894415/1‑150 (MQ=255) aTCGGAGTTGGTGGTGGCGCCGGCCGGGTATAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGc > 1:523855/1‑150 (MQ=255) ggAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAg > 1:734842/1‑150 (MQ=255) ggtggtggCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATgcg < 2:731243/150‑1 (MQ=255) gtggCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCa > 1:704235/1‑150 (MQ=255) cggGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGcc > 2:179264/1‑150 (MQ=255) ttGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCgg > 2:956118/1‑148 (MQ=255) ttGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCgg < 1:956118/148‑1 (MQ=255) gcgTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTg > 1:299957/1‑150 (MQ=255) cgTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTgc < 2:704235/150‑1 (MQ=255) gCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACa < 1:934539/129‑1 (MQ=255) gCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACa > 2:934539/1‑129 (MQ=255) gCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCAGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTgc < 2:1009740/133‑1 (MQ=255) gCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCAGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTgc > 1:1009740/1‑133 (MQ=255) ggCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTggg < 1:179264/150‑1 (MQ=255) aCGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCatgatgatg < 1:36530/150‑1 (MQ=255) cTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACg > 2:687831/1‑150 (MQ=255) tCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACgg > 1:268408/1‑150 (MQ=255) tCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACgg > 1:637685/1‑150 (MQ=255) gCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCAGCACGAGGTTGGGCATGATGATGGCACGGGCGaa < 2:179724/150‑1 (MQ=255) cggtcggtGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTAcgc < 1:894415/150‑1 (MQ=255) cggtcggtGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGCCAGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTAcgc < 2:938328/150‑1 (MQ=255) ggtcggtGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTAcgc < 1:394069/149‑1 (MQ=255) ggtcggtGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTAcgc > 2:394069/1‑149 (MQ=255) gtcggtGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACgcgc > 1:152842/1‑150 (MQ=255) gAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTc < 1:128155/150‑1 (MQ=255) ggTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCatga > 1:263824/1‑117 (MQ=255) ggTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCatga < 2:263824/117‑1 (MQ=255) gTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGAGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGcc > 1:860776/1‑150 (MQ=255) aGCACCATCAGCGGCTCGAAGCAGGGGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACg > 1:766204/1‑150 (MQ=255) cACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTa < 2:1060468/130‑1 (MQ=255) cACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTa > 1:1060468/1‑130 (MQ=255) cATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCATGGGCGGCACGAGGTTGGGCATCATGATCATATCGGCGAAGGTACGCGCCACGTCGCCAACGGtgtg < 1:834672/150‑1 (MQ=255) cATCAGCGGCTCGAAGCCGCGGGTGGCCGGGAGGGCCGCCAGGATGCGCTGACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGTGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGtgtg < 2:196899/150‑1 (MQ=255) aTCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTgg > 2:447632/1‑150 (MQ=255) tCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTggg < 2:152842/150‑1 (MQ=255) tCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTggg > 1:277753/1‑150 (MQ=255) gCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGaa < 2:30732/118‑1 (MQ=255) gCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTAACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGaa > 1:30732/1‑118 (MQ=255) gCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGATGCGGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCa > 1:812754/1‑150 (MQ=255) attctggCTGCCCGCCCGGCCGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGc > 1:751775/6‑150 (MQ=255) tggCTGCCCGCCCGGCCGGCAGCCGGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCatga > 2:416682/2‑91 (MQ=38) tggCTGCCCGCCCGGCCGGCAGCCGGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCatga < 1:416682/90‑1 (MQ=38) ggCTGCCCGCCCGGCCGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCACCAt > 1:1061644/1‑150 (MQ=255) gCTGCCCGCCCGGCCGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGc > 1:1019752/1‑122 (MQ=255) gCTGCCCGCCCGGCCGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGc < 2:1019752/122‑1 (MQ=255) tGCCCGCCCGGCCGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGACACCATcgc > 1:1067738/1‑150 (MQ=255) tGCCCGCCCGGCCGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGACACCATcgc < 2:1067738/150‑1 (MQ=255) gccggGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCACCATCGCGCAga < 2:637685/150‑1 (MQ=255) cgggcgggCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGgtgt > 2:447196/1‑122 (MQ=255) cgggcgggCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGgtgt < 1:447196/122‑1 (MQ=255) gggcAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCACCATCGCGCAGATGGAtgt > 1:428397/1‑150 (MQ=255) | GCCGGAATCGGAGTTGGTGGTGGCGCCGGCCGGGTACAACTTGGCGGCGTACACGAAACCGCTGGCCTTGGCCGCGCGGACGTCCTCGGGGCTGGTGCGGTCGGTGAGGTACAGCACCATCAGCGGCTCGAAGCGGCTGCCGGCCGGGCGGGCAGCCAGAATGCGCTCACGGTAGGCGCCGGCCTCGGTGACATTGCGCACGGGCGGCACGAGGTTGGGCATGATGATGGCACGGGCGAAGGTACGCGCCACGTCGCCAACGGTGTGGGGCAGGACGGCACCATCGCGCAGATGGATGT > NC_002947/1245195‑1245493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |