| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,272,648 | G→A | 11.1% | M112I (ATG→ATA) | PP_1113 → | pyridoxal‑phosphate dependent enzyme family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,272,648 | 0 | G | A | 11.1% | 88.8 / 3.8 | 36 | M112I (ATG→ATA) | PP_1113 | pyridoxal‑phosphate dependent enzyme family protein |
| Reads supporting (aligned to +/- strand): ref base G (17/15); new base A (2/2); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01 | |||||||||||
GCCACAAGTACCAGGTCGCCGTAGAGCTGGCGGTGGGGCTGAGCTTCAGCCAGACCAAGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATC > NC_002947/1272506‑1272795 | gCCACAAGTACCAGGTCGCCGTAGAGCTGGCGGTGGGGCTGAGCTTCAGCCAGACCAAGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGACATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCAcc < 1:281605/150‑1 (MQ=255) cacaAGTACCAGGTCGCCGTAGAGCTGGCGGTGGGGCTGAGCTTCAGCCAGACCAAGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGTTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCAccac < 1:260532/150‑3 (MQ=255) gTGGGGCTGAGCTTCAGCCAGACCAAGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATAc > 2:370802/1‑150 (MQ=255) ggggCTGAGCTTCAGCCAGACCAAGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACtt > 1:581454/1‑150 (MQ=255) cTGAGCTTCAGCCAGACCAAGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTac < 2:581454/150‑1 (MQ=255) tGAGCTTCAGCCAGACCAAGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTaca > 1:493747/1‑150 (MQ=255) ccggagagCAAGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAg > 2:300985/5‑150 (MQ=255) gCCAGACCAAGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATACATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGt > 1:601081/1‑150 (MQ=255) gACCAGGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCaa < 1:701684/123‑1 (MQ=255) gACCAAGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATTCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCaa > 2:701684/1‑123 (MQ=255) gACCAAGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTcaa < 2:493747/150‑1 (MQ=255) ccAAGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTcaaca > 1:488752/1‑150 (MQ=255) aaGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCGACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCaacaac > 2:202819/1‑150 (MQ=255) tCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGcc < 1:1127703/100‑1 (MQ=255) tCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGcc > 2:1127703/1‑100 (MQ=255) tgGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATACATCACCTGGATaa < 1:500758/88‑1 (MQ=255) tgGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATACATCACCTGGATaa > 2:500758/1‑88 (MQ=255) gtgtTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGc < 2:488752/150‑1 (MQ=255) tttCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCa > 1:105772/1‑150 (MQ=255) ggTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATACATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGATGGGCTTCGATGCCCACCgg < 2:601081/150‑2 (MQ=255) ccGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTATCa > 1:54763/1‑150 (MQ=255) aCTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTATCAGAc > 1:943262/1‑150 (MQ=255) tGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGTTGATGCATCCCCTGGATAACAAGGCCGCGCTGGGCAAGTCATACTTCTCCACCGCCCAGTTCAACACC‑CCGGAGGGAATCGAAGCCCATCGTTACCAGAccc > 1:1081807/1‑149 (MQ=255) ggCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGt < 2:105772/150‑1 (MQ=255) ggAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACt > 2:89226/1‑150 (MQ=255) tGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTC‑ACACCGCCAGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGc > 2:32309/1‑150 (MQ=255) ccGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGa < 1:276416/150‑1 (MQ=255) acacCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTATc < 2:1154936/112‑1 (MQ=255) acacCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTATc > 1:1154936/1‑112 (MQ=255) cAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTAt < 2:363890/106‑1 (MQ=255) cAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTAt > 1:363890/1‑106 (MQ=255) aaGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCa < 1:739808/150‑1 (MQ=255) aCGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTgg < 2:408953/39‑1 (MQ=255) aCGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTgg > 1:408953/1‑39 (MQ=255) tggtgATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGggcggc < 1:202819/150‑1 (MQ=255) aTGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATc < 1:89226/150‑1 (MQ=255) | GCCACAAGTACCAGGTCGCCGTAGAGCTGGCGGTGGGGCTGAGCTTCAGCCAGACCAAGCGGGATCTGCTGGAGTGTTTCGGTGCCCGACTGATCGGCAAGGAAATGCTGATGGCCGGTGCCACACCCAAGGACGTGGTGATGCATCACCTGGATAACCAGGCCGCGCTGGGCAAGTCATACTTCTACACCGACCAGTTCAACAAC‑CCGGTGGGCATCGAAGCCCATCGTTATCAGACCGCCAGTGAACTGGCCGTGCAACTGACCAACGCCGGGGCAGGGCGGCAGATC > NC_002947/1272506‑1272795 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |