Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,307,447 C→G 8.7% A59A (GCG→GCC)  livK ← branched‑chain amino acids ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,307,4470CG 8.7% 143.9 / 5.4 46A59A (GCG→GCC) livKbranched‑chain amino acids ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (23/19);  new base G (2/2);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

GCTGGGTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGC  >  NC_002947/1307305‑1307584
                                                                                                                                              |                                                                                                                                         
gctggGTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCGCGCCGCCCGCGGCGt                                                                                                                                    >  2:841470/1‑150 (MQ=255)
gctggGTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGt                                                                                                                                    <  1:841470/150‑1 (MQ=255)
  tggGTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGAGTTg                                                                                                                                  >  1:304997/1‑150 (MQ=255)
                    aGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGgcttgc                                                                                                                <  2:1062481/150‑1 (MQ=255)
                      gTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGa                                                                                                                        <  1:1035382/140‑1 (MQ=255)
                      gTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGa                                                                                                                        >  2:1035382/1‑140 (MQ=255)
                                    aCTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGtt                                                                                                                                   <  1:59876/115‑1 (MQ=255)
                                    aCTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGtt                                                                                                                                   >  2:59876/1‑115 (MQ=255)
                                         aCGCCGTCGTTGACCACTTTGTCGGCGACTCCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCAtt                                                                                           >  2:137503/1‑149 (MQ=255)
                                             cgtcgtTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGc                                                                                       >  1:365869/1‑150 (MQ=255)
                                              gtcgtTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGcc                                                                                      <  1:779325/150‑1 (MQ=255)
                                                  ttGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTAc                                                                                  >  2:698469/1‑150 (MQ=255)
                                                     aCCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTgg                                                                               <  2:706584/150‑1 (MQ=255)
                                                      ccACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGGCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTggg                                                                              >  1:59259/1‑150 (MQ=255)
                                                       cACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTggg                                                                              >  2:16161/1‑149 (MQ=255)
                                                       cACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTggg                                                                              <  1:16161/149‑1 (MQ=255)
                                                        aCTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGATc                                                                            >  1:52491/1‑150 (MQ=255)
                                                             gttgGCCACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGATTCAAGGATATAAACGTCCACGCCTCCCGCGGCGTGGCTATCCTTGATGGCTTGCTTGTCGCGTAGGAACTGCATGTCGCCGTACTGGGTCACggg                                                                       <  1:574710/150‑1 (MQ=255)
                                                                 gCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGAccc                                                                   <  2:981683/150‑1 (MQ=255)
                                                                    aCTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTc                                                                >  1:754866/1‑150 (MQ=255)
                                                                        ccACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTgg                                                            <  1:797353/150‑1 (MQ=255)
                                                                               cTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGc                                                     >  1:624734/1‑150 (MQ=255)
                                                                                  tttAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGt                                                                             >  1:1145614/1‑123 (MQ=255)
                                                                                  tttAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGt                                                                             <  2:1145614/123‑1 (MQ=255)
                                                                                  tttAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGAt                                                  >  1:408750/1‑150 (MQ=255)
                                                                                         tCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATc                                           >  2:342816/1‑150 (MQ=255)
                                                                                              ggCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTTGGTCCCGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGAt                                      <  1:698469/150‑1 (MQ=255)
                                                                                              ggCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGAt                                      <  1:342816/150‑1 (MQ=255)
                                                                                              ggCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGAt                                      <  1:287240/150‑1 (MQ=255)
                                                                                                 gtcgtcgtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGc                                                                                              <  1:681488/91‑1 (MQ=255)
                                                                                                 gtcgtcgtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGc                                                                                              >  2:681488/1‑91 (MQ=255)
                                                                                                     tcgtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACg                                                                         <  2:200340/108‑1 (MQ=255)
                                                                                                     tcgtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACg                                                                         >  1:200340/1‑108 (MQ=255)
                                                                                                          ttCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCggcggc                          >  1:707962/1‑150 (MQ=255)
                                                                                                                   ttCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTTATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtg                 >  1:154687/1‑150 (MQ=255)
                                                                                                                        gCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGGTGCCGATCTTGATGGTATCGGCGGCAAACGAAtggctgg            >  1:969715/1‑150 (MQ=255)
                                                                                                                        gCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggctgg            <  2:707962/150‑1 (MQ=255)
                                                                                                                        gCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggctgg            <  2:624734/150‑1 (MQ=255)
                                                                                                                          aTCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATggctggct          >  1:351128/1‑150 (MQ=255)
                                                                                                                            cTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTg                                                             >  1:1153425/1‑97 (MQ=255)
                                                                                                                            cTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTg                                                             <  2:1153425/97‑1 (MQ=255)
                                                                                                                               gatatcaaCGCCGCGGGCGGCGTGGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggct              <  2:37055/134‑1 (MQ=255)
                                                                                                                               gatatcaaCGCCGCGGGCGGCGTGGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggct              >  1:37055/8‑141 (MQ=255)
                                                                                                                                  aTCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGc  >  2:1049487/1‑150 (MQ=255)
                                                                                                                                    caaCGCCGCGGGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAAc                      >  1:889957/3‑128 (MQ=255)
                                                                                                                                    caaCGCCGCGGGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAAc                      <  2:889957/126‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                         
GCTGGGTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGC  >  NC_002947/1307305‑1307584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑