Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,389,952 C→G 8.5% A23G (GCC→GGC)  PP_1212 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,389,9520CG 8.5% 139.7 / 3.2 46A23G (GCC→GGC) PP_1212hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (24/18);  new base G (2/2);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TTGCTAACCATATATAATCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAGGCGGCGACAAGGC  >  NC_002947/1389814‑1390099
                                                                                                                                          |                                                                                                                                                   
ttgctAACCATATATAATCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGa                                                                                                                                          >  1:746245/1‑150 (MQ=255)
        cctctataATCCCCAATTCTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTCCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGc                                                                                                                                  >  2:565680/5‑150 (MQ=255)
         atatatAATCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCTCTGCTCTGATCGATTGcc                                                                                                                                 <  2:897631/150‑1 (MQ=255)
                                 cccccGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGAt                                                                                                                                     >  1:189248/1‑122 (MQ=255)
                                 cccccGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGAt                                                                                                                                     <  2:189248/122‑1 (MQ=255)
                                      ggCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGGCGCGGCGCTGATcttc                                                                                                                                     >  1:519586/1‑114 (MQ=255)
                                      ggCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGGCGCGGCGCTGATcttc                                                                                                                                     <  2:519586/117‑4 (MQ=255)
                                            cagcGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGctgctg                                                                                              >  1:1096543/1‑150 (MQ=255)
                                             agcGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCa                                                                                                                       >  1:178209/1‑124 (MQ=255)
                                             agcGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCa                                                                                                                       <  2:178209/124‑1 (MQ=255)
                                                      gTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATc                                                                                                                                        >  1:736443/1‑98 (MQ=255)
                                                      gTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATc                                                                                                                                        <  2:736443/98‑1 (MQ=255)
                                                            gagagaCACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCgg                                                                                                               >  2:398492/1‑117 (MQ=255)
                                                            gagagaCACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCgg                                                                                                               <  1:398492/117‑1 (MQ=255)
                                                            gagagaCACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTc                                                                              >  1:899131/1‑150 (MQ=255)
                                                              gagaCACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGc                                                                            <  2:899131/150‑1 (MQ=255)
                                                                    cccATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGc                                                                      <  2:977218/150‑1 (MQ=255)
                                                                     ccATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAg                                                                       <  1:593482/148‑1 (MQ=255)
                                                                     ccATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAg                                                                       >  2:593482/1‑148 (MQ=255)
                                                                     ccATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCg                                                                     <  2:486099/150‑1 (MQ=255)
                                                                     ccATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCg                                                                     >  1:486099/1‑150 (MQ=255)
                                                                      cATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCgg                                                                    <  2:1096543/150‑1 (MQ=255)
                                                                           cccTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGaaaaa                                                                                                     >  2:1059602/1‑112 (MQ=255)
                                                                           cccTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGaaaaa                                                                                                     <  1:1059602/112‑1 (MQ=255)
                                                                               ttATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTg                                                           <  2:746245/150‑1 (MQ=255)
                                                                                  tGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCCCCGCTGATAGATTCCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTa                                                        >  1:450160/1‑150 (MQ=255)
                                                                                        tCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAAc                                                  >  1:567676/1‑150 (MQ=255)
                                                                                           aTGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGa                                               >  1:312029/1‑150 (MQ=255)
                                                                                                 gTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCa                                                                                     >  2:302149/1‑106 (MQ=255)
                                                                                                 gTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCa                                                                                     <  1:302149/106‑1 (MQ=255)
                                                                                                    cTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACt                                                                                                           >  2:1059625/1‑81 (MQ=255)
                                                                                                    cTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACt                                                                                                           <  1:1059625/81‑1 (MQ=255)
                                                                                                      gCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCg                                    <  2:642769/150‑1 (MQ=255)
                                                                                                             ccGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACtt                                            <  2:1051896/135‑1 (MQ=255)
                                                                                                             ccGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACtt                                            >  1:1051896/1‑135 (MQ=255)
                                                                                                                cATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGaaaaa                          >  1:726267/1‑150 (MQ=255)
                                                                                                                          ccgcAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCa                >  1:167739/3‑150 (MQ=255)
                                                                                                                           tgcAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAg               >  1:889264/1‑150 (MQ=255)
                                                                                                                            gcAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAgg              >  2:692475/1‑150 (MQ=255)
                                                                                                                             cAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCATGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAggc             <  1:973935/150‑1 (MQ=255)
                                                                                                                              agaagaTCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCttt                                                                               >  1:481295/1‑83 (MQ=255)
                                                                                                                              agaagaTCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCttt                                                                               <  2:481295/83‑1 (MQ=255)
                                                                                                                                tcgaTCAGCGGCGCGGCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAggcg            >  1:536401/3‑148 (MQ=255)
                                                                                                                                tcgaTCAGCGGCGCGGCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAggcg            <  2:536401/146‑1 (MQ=255)
                                                                                                                                 agaTCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAGgcggcg         >  1:679459/1‑150 (MQ=255)
                                                                                                                                  gaTCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAGGCGGCGa        >  1:551581/1‑150 (MQ=255)
                                                                                                                                        cgccgcgccgCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAGGCGGCGACAAGGc  <  2:312029/150‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
TTGCTAACCATATATAATCCCGCTTTTTGCTGCCCCCCGGCAGCCAGCGTTATGGTTGACGAGAGACACCCATGCCCCTTTATGACTATCAATGTGCGTCCTGCGAGCACCGCATGGAAGTGCTGCAGAAGATCAGCGCCGCGCCGCTGATCGATTGCCCGGCCTGTCAGGCGCCGGCACTGAAAAAGCTGCTGTCGGTGCCAGGCTTTCGCCTGAGCGGTAACGGTTGGTACGAAACCGACTTCAAGACCGGGGCGAAAAAGAATCTGGCAGGCGGCGACAAGGC  >  NC_002947/1389814‑1390099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑