Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,400,878 C→A 8.2% intergenic (+35/‑134) ybgF → / → queE TolQRA transport system periplasmic protein/7‑carboxy‑7‑deazaguanine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,400,8780CA 8.2% 141.8 / 3.1 49intergenic (+35/‑134)ybgF/queETolQRA transport system periplasmic protein/7‑carboxy‑7‑deazaguanine synthase
Reads supporting (aligned to +/- strand):  ref base C (22/23);  new base A (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

GAGCGCCGTATGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGACACA  >  NC_002947/1400730‑1401023
                                                                                                                                                    |                                                                                                                                                 
gAGCGCCGTATGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGcc                                                                                                                                                  >  2:550219/1‑150 (MQ=255)
gAGCGCCGTATGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGcc                                                                                                                                                  >  2:39450/1‑150 (MQ=255)
 aGCGCCGTATGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCa                                                                                                                                                 <  1:550219/150‑1 (MQ=255)
                aTACCGACAAGGTAAAAGGCATCCTGCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCCCTCTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTc                                                                                                                                  <  2:72770/150‑1 (MQ=255)
                   ccGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGtt                                                                                                                               <  1:39450/150‑1 (MQ=255)
                           gTAAAAGGCATCCTTCCGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCAc                                                                                                                       <  2:315973/150‑1 (MQ=255)
                             aaaaGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTGAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTCGTTAGAATCACTg                                                                                                                     >  1:458126/1‑150 (MQ=255)
                                   cATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCttt                                                                                                               <  2:631709/150‑1 (MQ=255)
                                     tCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGTGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCttttt                                                                                                             <  1:1154591/150‑1 (MQ=255)
                                        ttCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGAGCCCTAAAGAAGCTCACACGGTTAGCGCTGTAGCGCGCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTCGTTAGAATCACTGCCCTTTTTTCg                                                                                                          >  1:448861/1‑150 (MQ=255)
                                             cagGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAac                                                                                                     >  2:987034/1‑150 (MQ=255)
                                                   gTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTcgcc                                                                                                                                           <  2:442344/106‑2 (MQ=255)
                                                   gTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTcgcc                                                                                                                                           >  1:442344/1‑105 (MQ=255)
                                                       cccAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCtgctgc                                                                                           <  1:1052223/150‑1 (MQ=255)
                                                       cccAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCtgctgc                                                                                           >  2:1052223/1‑150 (MQ=255)
                                                        ccAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACCCGCTGCTGCg                                                                                          >  1:654079/1‑150 (MQ=255)
                                                            tACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAACCCCGCGCCAGTCGCGGGCCTTCTCGTCAAAATCACTGCCGTTGTTTGTTAAACACGCTGGTGCGGATa                                                                                      >  2:132367/1‑150 (MQ=255)
                                                                ccGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATaa                                                                                     <  1:432204/147‑1 (MQ=255)
                                                                ccGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATaa                                                                                     >  2:432204/1‑147 (MQ=255)
                                                                ccGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGc                                                                                  >  2:640503/1‑150 (MQ=255)
                                                                 cGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACg                                                                                                  >  1:420182/1‑133 (MQ=255)
                                                                 cGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACg                                                                                                  <  2:420182/133‑1 (MQ=255)
                                                                       ctctGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTcgc                                                                                                                                            >  1:4507/1‑85 (MQ=255)
                                                                       ctctGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTcgc                                                                                                                                            <  2:4507/85‑1 (MQ=255)
                                                                        tctGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCTCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGa                                                                          <  2:154277/150‑1 (MQ=255)
                                                                                  aaCTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACa                                                                <  2:1013106/150‑1 (MQ=255)
                                                                                        cccAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCt                                                          <  1:547422/150‑1 (MQ=255)
                                                                                         ccAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTg                                                         >  1:355035/1‑150 (MQ=255)
                                                                                         ccAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTg                                                         >  1:1110541/1‑150 (MQ=255)
                                                                                          cAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCAAGCCCGCGTCAGTTCCTGa                                                        <  2:379996/150‑1 (MQ=255)
                                                                                            gCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGa                                                                          >  2:1086262/1‑130 (MQ=255)
                                                                                            gCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGa                                                                          <  1:1086262/130‑1 (MQ=255)
                                                                                                             tctAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCCCTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGc                                                            >  1:466574/1‑127 (MQ=255)
                                                                                                             tctAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTTGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGtt                                     >  2:1051773/1‑150 (MQ=255)
                                                                                                             tctAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAACCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGc                                                            <  2:466574/127‑1 (MQ=255)
                                                                                                               tAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTa                                   >  1:717766/1‑150 (MQ=255)
                                                                                                                     ttGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAGGCACGCTGCTGCGGATAACGCTATGTNGAGTCCNCGACAGTGCCTGNCGGAGGCGGACAGCCTGTTTAGCTGTc                             <  1:1051773/150‑1 (MQ=255)
                                                                                                                           ctctACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTc                                                                            >  1:1203696/4‑97 (MQ=255)
                                                                                                                           cTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTc                                                                            <  2:1203696/97‑1 (MQ=255)
                                                                                                                           cTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGccc                       <  2:654079/150‑1 (MQ=255)
                                                                                                                               aCCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTgg                   >  1:851947/1‑150 (MQ=255)
                                                                                                                                ccGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGc                  <  1:132367/150‑1 (MQ=255)
                                                                                                                                       gaaaaaaCCCGCGACTGGCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCCACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCt                                <  1:475465/125‑1 (MQ=255)
                                                                                                                                       gaaaaaaCCCGCGACTGGCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCCACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCt                                >  2:475465/5‑129 (MQ=255)
                                                                                                                                       aaagaaaCCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATg           <  1:640503/150‑1 (MQ=255)
                                                                                                                                       aaagaaaCCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTGTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATg           >  1:26574/1‑150 (MQ=255)
                                                                                                                                           aaaCCCGCGACTGGCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAg       >  2:837610/1‑150 (MQ=255)
                                                                                                                                            aaCCCGCGACTGGCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGa      <  1:837610/150‑1 (MQ=255)
                                                                                                                                             aCCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCa                            <  2:1071794/127‑1 (MQ=255)
                                                                                                                                             aCCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCa                            >  1:1071794/1‑127 (MQ=255)
                                                                                                                                                cgcgCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGacacc  >  1:170826/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GAGCGCCGTATGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGACACA  >  NC_002947/1400730‑1401023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑