Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,405,564 C→A 5.8% V42F (GTC→TTC)  PP_1228 ← methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,405,5640CA 5.8% 210.0 / 3.0 69V42F (GTC→TTC) PP_1228methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base C (29/36);  new base A (2/2);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-01

TCAGCACCTGTACCAGGCGGGCAAAGCCATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATGGGGGCTTTCCTTTTCGAACAAATGC  >  NC_002947/1405417‑1405713
                                                                                                                                                   |                                                                                                                                                     
ccAGCACCTGTACCCGGCGGGCCCAGCCTTTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCa                                                                                                                                                     <  2:316853/149‑1 (MQ=255)
 cAGCACCTGTACCAGGCGGGCAAAGCCATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAg                                                                                                                                                    >  2:212027/1‑150 (MQ=255)
  aGCACCTGTACCAGGCGGGACAAGCCCTTGCTGTACTGGTAGGCGTTCAGGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAgg                                                                                                                                                   <  2:316133/150‑1 (MQ=255)
      ccTGTACCAGGCGGGCAAAGCCATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGAACAGggcg                                                                                                                                                <  1:84772/149‑1 (MQ=255)
      ccTGTACCAGGCGGGCAAAGCCATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGAACAGggcg                                                                                                                                                <  1:84800/149‑1 (MQ=255)
      ccTGTACCAGGCGGGCAAAGCCATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGAACAGggcg                                                                                                                                                >  2:84772/1‑149 (MQ=255)
      ccTGTACCAGGCGGGCAAAGCCATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGAACAGggcg                                                                                                                                                >  2:84800/1‑149 (MQ=255)
       cTGTACCAGGCGGGCAAAGCCATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGggcggc                                                                                                                                              >  1:1060888/1‑150 (MQ=255)
       cTGTACCAGGCGCGCAACGCCATTGCCGTACTGGTAGGCGTCCATGAAGTCAACCTCGATACCCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCTGGCGCTGCCTTCGAGCATGGCAGTGTTGGCCTGGTTTACCAgggaagc                                                                                                                                              >  2:296403/1‑146 (MQ=255)
           aCCAGGCGGGCAAAGCCATTGCCGTACTGGTAGGCGTCCATGACGTGAGACTAGAGACTCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTg                                                                                                                                          <  2:31828/150‑1 (MQ=255)
              aGGCGGGCAAAGCCATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGa                                                                                                                                       >  2:592378/1‑150 (MQ=255)
               ggCGGGCAAAGCCATTGTCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGAt                                                                                                                                      <  1:61273/150‑1 (MQ=255)
               ggCGGGCAAAGCCATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGAt                                                                                                                                      <  1:346387/150‑1 (MQ=255)
                  gggCAAAGCCATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATggg                                                                                                                                   <  2:721587/150‑1 (MQ=255)
                   ggCAAAGCCAGTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTGCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGc                                                                                                                                  <  1:51265/150‑1 (MQ=255)
                       aaGCCATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTgg                                                                                                                              <  1:263984/150‑1 (MQ=255)
                        aGCCATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTggg                                                                                                                             <  2:922588/150‑1 (MQ=255)
                          ccATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTc                                                                                                                           >  1:222735/1‑150 (MQ=255)
                           cATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCa                                                                                                                          <  1:659861/150‑1 (MQ=255)
                              tGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCg                                                                                                                       <  1:608039/150‑1 (MQ=255)
                                ccGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGaa                                                                                                                     <  1:301021/150‑1 (MQ=255)
                                  gTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGt                                                                                                                   <  1:650751/150‑1 (MQ=255)
                                     cTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGc                                                                                                                <  1:733915/150‑1 (MQ=255)
                                     cTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGc                                                                                                                <  2:222735/150‑1 (MQ=255)
                                            gCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTGGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGc                                                                                                         >  1:1150888/1‑150 (MQ=255)
                                              gTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCaa                                                                                                       >  1:762743/1‑150 (MQ=255)
                                                ccATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAgg                                                                                                     <  2:893980/150‑1 (MQ=255)
                                                ccATGAAGTAGCGCTGGATACGCAAGGCCTGGATTTGGGCGTGGGCCTGCAGGCGCTGGCGGGAGCTGTCTTTGAGCATGGCAGTGTTGGCCTTGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGTGAAGTGGCGGcccgtaggg                                                                                                     >  2:354847/1‑143 (MQ=255)
                                                  aTGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCg                                                                                                   >  2:601734/1‑150 (MQ=255)
                                                        tAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATg                                                                                             >  1:290086/1‑150 (MQ=255)
                                                           cgcTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGcc                                                                                          <  2:1060888/150‑1 (MQ=255)
                                                                     gCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCCGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGGCAGCGAAGTTGCGACCAGCAAGGCGACAATGGCCAGCAGGcaca                                                                                >  1:603581/1‑150 (MQ=255)
                                                                       aaGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGAc                                                                                                 <  2:551456/131‑1 (MQ=255)
                                                                       aaGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGAc                                                                                                 >  1:551456/1‑131 (MQ=255)
                                                                          gCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCg                                                                           >  1:321095/1‑150 (MQ=255)
                                                                            cTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATg                                                                                             <  2:231471/130‑1 (MQ=255)
                                                                            cTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATg                                                                                             >  1:231471/1‑130 (MQ=255)
                                                                             tGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCt                                                                                                                                >  2:1036602/1‑94 (MQ=255)
                                                                             tGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCt                                                                                                                                <  1:1036602/94‑1 (MQ=255)
                                                                              gggTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCga                                                                       >  2:225316/1‑150 (MQ=255)
                                                                               ggTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCgag                                                                      <  1:755631/150‑1 (MQ=255)
                                                                                    cGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAgtg                                                                 <  2:1150888/150‑1 (MQ=255)
                                                                                     ggCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAgtgt                                                                >  1:557939/1‑150 (MQ=255)
                                                                                             ccTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGATGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTcc                                                        >  2:515269/1‑150 (MQ=255)
                                                                                                   ggCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACtgga                                                  <  1:601734/150‑1 (MQ=255)
                                                                                                   ggCGCTGGCGGGCGCTGTCGTCGAGCTTGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGCGCTTCGGGCAGCTAAGTGGCGACCAGCAAGGCGACAAGGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACtgga                                                  <  2:402215/150‑1 (MQ=255)
                                                                                                    gcgcTGGTGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCCGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACtggat                                                 <  2:119901/150‑1 (MQ=255)
                                                                                                    gcgcTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACtggat                                                 >  2:1180307/1‑150 (MQ=255)
                                                                                                      gcTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACtggatgg                                               <  1:592378/150‑1 (MQ=255)
                                                                                                         ggcgggcgCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGgaga                                            <  2:557939/150‑1 (MQ=255)
                                                                                                          gcgggcgCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACtgga                                                  <  1:683289/143‑1 (MQ=255)
                                                                                                          gcgggcgCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACtgga                                                  >  2:683289/1‑143 (MQ=255)
                                                                                                              gcgcTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAACGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGa                                       <  1:182869/150‑1 (MQ=255)
                                                                                                                gcTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACg                                     >  2:1180642/1‑150 (MQ=255)
                                                                                                                   gTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAg                                  <  2:290086/150‑1 (MQ=255)
                                                                                                                    tCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGc                                 <  1:486979/150‑1 (MQ=255)
                                                                                                                      ttCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGTTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAATATGATCTTCCACGGGATGGAGTGGCGACGCAGCgg                               >  2:315636/1‑150 (MQ=255)
                                                                                                                          aGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATg                           >  2:427369/1‑150 (MQ=255)
                                                                                                                          aGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATg                           <  1:1180642/150‑1 (MQ=255)
                                                                                                                               ggCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACtgga                                                  >  1:402652/1‑122 (MQ=255)
                                                                                                                               ggCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACtgga                                                  <  2:402652/122‑1 (MQ=255)
                                                                                                                               ggCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATGGGGGc                      >  2:1024428/1‑150 (MQ=255)
                                                                                                                                  aGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATGGGGGCttt                   <  1:296403/150‑1 (MQ=255)
                                                                                                                                    tgtTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATGGGGGCTTTcc                 >  2:928640/1‑150 (MQ=255)
                                                                                                                                      ttGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATGGGGGCTTTCCtt               >  2:64859/1‑150 (MQ=255)
                                                                                                                                      ttGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATGGGGGCTTTCCtt               >  2:867230/1‑150 (MQ=255)
                                                                                                                                        ggCCTGGTTGACCAGGGCGGGGCTGGGAGGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATGGGGGCTTTCCtttt             <  2:321095/150‑1 (MQ=255)
                                                                                                                                         gCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGGCCAGTAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCCCTGGATGGAGAGGCGACGCAGCGGCATGGGGGCTTTCCTTTTc            >  1:689176/1‑150 (MQ=255)
                                                                                                                                            tcgTTGACCCGGGCGGCGCTGCCATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATGGGGGCTTTCCTTTTCGaa         <  1:354847/148‑1 (MQ=255)
                                                                                                                                                   ccAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATGGGGGCttt                   <  2:484465/133‑1 (MQ=255)
                                                                                                                                                   ccAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATGGGGGCttt                   >  1:484465/1‑133 (MQ=255)
                                                                                                                                                   ccAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATGGGGGCTTTCCTTTTCGAACAAATGc  <  2:603581/150‑1 (MQ=255)
                                                                                                                                                   ccAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATGGGGGCTTTCCTTTTCGAACAAATGc  >  1:630746/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TCAGCACCTGTACCAGGCGGGCAAAGCCATTGCCGTACTGGTAGGCGTCCATGAAGTAGCGCTGGATACGCAAGGCCTGGGTTTCGGCGTGGGCCTGCAGGCGCTGGCGGGCGCTGTCTTCGAGCATGGCAGTGTTGGCCTGGTTGACCAGGGCGGCGCTGCGATGGGCCTGGGTCAGCGAAGTGGCGACCAGCAAGGCGACAATGGCCAGCAGGCACAGGCCGGCGAGGAGTGTGATCTTCCACTGGATGGAGAGGCGACGCAGCGGCATGGGGGCTTTCCTTTTCGAACAAATGC  >  NC_002947/1405417‑1405713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑