| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,409,146 | G→A | 11.1% | intergenic (+84/‑191) | PP_1230 → / → nadA | hypothetical protein/quinolinate synthase iron‑sulfur cluster subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,409,146 | 0 | G | A | 11.1% | 138.1 / 7.6 | 54 | intergenic (+84/‑191) | PP_1230/nadA | hypothetical protein/quinolinate synthase iron‑sulfur cluster subunit |
| Reads supporting (aligned to +/- strand): ref base G (28/20); new base A (3/3); total (31/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
GACCGTTGAAGAATTTGCCATGTTCATGGCGGCGCATCTGGGGGCCCTGTCGCCGCAGGGGTGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGTCCGGCACCCTTAGCCGATCTGCCAGGGCCGGGTGATAC > NC_002947/1408999‑1409295 | gACCGTTGAAGAATTTGCCATGTTCATGGCGGCGCATCTGGGGGCCCTGTCGCCGCAGGGGTGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGgg > 1:1141627‑M2/1‑148 (MQ=255) ttGAAGAATTTGCCATGTTCATGGCGGCGCATCTGGGGGCCCTGTCGCCGCAGGGGTGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGcccac > 2:606328/1‑148 (MQ=255) ttGAAGAATTTGCCATGTTCATGGCGGCGCATCTGGGGGCCCTGTCGCCGCAGGGGTGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGc > 2:1032164/1‑150 (MQ=255) ttGAAGAATTTGCCATGTTCATGGCGGCGCATCTGGGGGCCCTGTCGCCGCAGGGGTGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGGGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGGTCACCCGCGAAGGGGCCCGc > 1:738875/1‑150 (MQ=255) gaaTTTGCCATGTTCATGGCGGCGCATCTGGGGGCCCTGTCGCCGCAGGGGTGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAg < 2:940965/150‑1 (MQ=255) aTTTGCCATGTTCATGGCGGCGCATCTGGGGGCCCTGTCGCCGCAGGGGTGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCa < 1:473794/150‑1 (MQ=255) tGGCGGCGCATCTGGGGGCCCTGTCGCCGCAGGGGTGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCt > 2:1196502/1‑150 (MQ=255) tCTGGGGGCCCTGTCGCCGCAGGGGTGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCtt < 2:367203/141‑1 (MQ=255) tCTGGGGGCCCTGTCGCCGCAGGGGTGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCtt > 1:367203/1‑141 (MQ=255) gccgcAGGGGTGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACaaaaa < 2:997792/120‑1 (MQ=255) gccgcAGGGGTGATGGTTTCTCCTGTTGCAGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACaaaaa > 1:997792/1‑120 (MQ=255) ggggTGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTGCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGATGGATTGTCTGTAGCCTTCg > 1:880322/1‑150 (MQ=255) gggATTGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGcc > 1:347640/3‑150 (MQ=255) tGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCt > 2:901785/1‑150 (MQ=255) aTGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGTGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTcc > 2:1061908/1‑150 (MQ=255) ctcCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGataata > 1:28171/1‑150 (MQ=255) ccTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATaataat < 1:1196502/150‑1 (MQ=255) cTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATc > 2:366670/1‑150 (MQ=255) tGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGCGGATTTCACAGTGTACGGGCGGGTTCACCCGCGAAGGGGCGCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTACGGCCCCCGCTACTAATcg > 2:532815/1‑149 (MQ=38) ggCCTCTGCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGccc < 2:1113341/150‑1 (MQ=255) gCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCg > 2:451510/1‑150 (MQ=255) ctctTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCa < 1:901785/150‑1 (MQ=255) tctTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCag > 2:177512/1‑150 (MQ=255) ttCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGggccgcaacaggagaaaccatcacccctgcggcgacagggcccccagatgcgccgccatgaacatggcaaattcttcaacg < 2:385462‑M2/143‑82 (MQ=255) ttCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGggccgcaacaggagaaaccatcacccctgcggcgacagggcccccagatgcgccgccatgaacatggcaaattcttcaacg > 1:385462‑M2/1‑62 (MQ=255) ttCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCagag > 2:395038/1‑150 (MQ=255) gTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCACGAAGGGGCCCCCGCAAGCCCTCCCACAACCCCTTTAGTAATCGCGGAATTGTCTGTAGCGTTCCGCCTCCGATAATATTCCGCGCCCGCAGACGGCCGta > 2:359854/1‑149 (MQ=255) aCCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGAggccgcaacaggagaaaccatcacccctgcggcgacagggcccccagatgcgccgccatgaacatggcaaattcttcaacggtcatttttc < 1:235935‑M2/142‑92 (MQ=255) aCCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGAggccgcaacaggagaaaccatcacccctgcggcgacagggcccccagatgcgccgccatgaacatggcaaattcttcaacggtcatttttc > 2:235935‑M2/1‑51 (MQ=255) ccGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGggccgcaacaggagaaaccatcacccctgcggcgacagggcccccagatgcgccgccatgaacatggcaaattcttcaa < 2:1159130‑M2/128‑80 (MQ=255) ccGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGggccgcaacaggagaaaccatcacccctgcggcgacagggcccccagatgcgccgccatgaacatggcaaattcttcaa > 1:1159130‑M2/1‑49 (MQ=255) ccGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGggccgcaacaggagaaaccatcacccctgcggcgacagggcccccagatgc < 2:743882‑M2/100‑52 (MQ=255) ccGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGggccgcaacaggagaaaccatcacccctgcggcgacagggcccccagatgc > 1:743882‑M2/1‑49 (MQ=255) ccGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGAggccgcaacaggagaaaccatcacccctgcggcgacagggcccccagatgc < 2:665039‑M2/100‑52 (MQ=255) ccGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGAggccgcaacaggagaaaccatcacccctgcggcgacagggcccccagatgc > 1:665039‑M2/1‑49 (MQ=255) gCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCaa < 1:1032164/150‑1 (MQ=255) gCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGAggccgcaacaggagaaaccatcacccctgcggcgacagggcccccagatgcgccgccatgaacatggcaaattcttc < 2:615024‑M2/124‑78 (MQ=255) gCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGAggccgcaacaggagaaaccatcacccctgcggcgacagggcccccagatgcgccgccatgaacatggcaaattcttc > 1:615024‑M2/1‑47 (MQ=255) gCTCCGACGAGGATTTCACAGTGTAGGGGCGGGTCCACCCGCGCAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCaa < 2:738875/150‑1 (MQ=255) cTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGTGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAAc < 2:581630/150‑1 (MQ=255) tACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTTTGTACCCTTTTGTCTCGGGTAAGAAGGCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGc < 1:511238/150‑1 (MQ=255) gAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGt > 2:103947/1‑150 (MQ=255) ggATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGTcc < 2:44165/150‑1 (MQ=255) gATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGTCCg > 2:225517/1‑150 (MQ=255) agagtAGGGGCCGGTTCACCCGCCAGGGGGCCCCCACAAGCACGACAAAAACCCCTTCAGTAATCGCTGGATGGCCTGTAGCCTTCGCCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGTCCGGCACCCtt > 2:199521/4‑150 (MQ=255) tgtAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCg < 2:174253/85‑1 (MQ=255) tgtAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCg > 1:174253/1‑85 (MQ=255) gtAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGTCCGGCACCCTTAGc < 1:532815/150‑1 (MQ=255) ggggCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGTCCGGCACCCTTAGCCGa > 1:226669/1‑150 (MQ=255) tCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGTCCGGCACCCTTAGCCGATCTGCCAgg < 1:395038/150‑1 (MQ=255) ccGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGTCCGGCACCCTTAGCCGATCTGCCAGGGCCg > 2:244456/1‑150 (MQ=255) ccGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGTCCGGCACCCTTAGCCGATCTGCCAGGGCCg > 1:611228/1‑150 (MQ=255) aaGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGTCCGGCACCCTTAGCCGATCTGCCAGGGCCGGGTGa < 1:177512/150‑1 (MQ=255) aGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGTCCGGCACCCTTAGCCGATCTGCCAGGGCCGGGTGAt < 1:606328/150‑1 (MQ=255) gggCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGTCCGGCACCCTTAGCCGATCTGCCAGGGCCGGGTGATac < 1:103947/150‑1 (MQ=255) | GACCGTTGAAGAATTTGCCATGTTCATGGCGGCGCATCTGGGGGCCCTGTCGCCGCAGGGGTGATGGTTTCTCCTGTTGCGGCCTCTTCGCGGGTGAACCCGCTCCTACGAGGATTTCACAGTGTAGGGGCGGGTTCACCCGCGAAGGGGCCCGCACAAGCACTACAAAAACCCCTTTAGTAATCGCTGGATTGTCTGTAGCCTTCGGCCTCCGATAATAATCCGCGCCCGCAGAGGGCCGTGGCGGTCAACATGCCGTCCGGCACCCTTAGCCGATCTGCCAGGGCCGGGTGATAC > NC_002947/1408999‑1409295 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |