Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,425,657	T→C	7.0%	intergenic (+52/‑453)	PP_1249 → / → PP_1250	DUF4223 domain‑containing protein/group II intron‑encoding maturase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,425,657	0	T	C	7.0%	206.5 / 3.0	71	intergenic (+52/‑453)	PP_1249/PP_1250	DUF4223 domain‑containing protein/group II intron‑encoding maturase
Reads supporting (aligned to +/- strand):  ref base T (33/33);  new base C (2/3);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

CTGCACCTACGACTACCTGTTCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGG  >  NC_002947/1425512‑1425805                                                                                                                                                   |                                                                                                                                                     cTGCACCTACGACTACCTGTTCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATg                                                                                                                                                  <  2:1056163/150‑1 (MQ=255) cTGCACCTACGACTACCTGTTCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAACAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATg                                                                                                                                                  <  2:1055803/150‑1 (MQ=255)      ccTCCGACTACCTGTTCCACCCTTCGGTTTCCATCTCTAAGTTCAT‑CGGTGGCTGCTGCCCGTTCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGAACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACAt                                                                                                                                             <  1:314029/150‑1 (MQ=255)             ctcccTGTCCCACCCTTCGGTTTCCATCTCCAAGACAATACGGT‑GCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGCCATGTGCATGACCggg                                                                                                                                      <  1:919127/147‑1 (MQ=255)              tACCTGTTCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCgggg                                                                                                                                     >  2:1084295/1‑150 (MQ=255)                 cTGTTCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGtt                                                                                                                                  >  2:355797/1‑150 (MQ=255)                  tGTTCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGG‑CCCCGGTCATGCACATGGCCGGGGGtttt                                                                                                                                >  2:1111155/1‑150 (MQ=255)                    ttCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGttttt                                                                                                                               >  1:925834/1‑150 (MQ=255)                                      aTCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGgc                                                                                                             <  1:944897/150‑1 (MQ=255)                                       tctcCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGgcg                                                                                                            >  1:674418/1‑150 (MQ=255)                                         tcCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGcgcg                                                                                                          >  2:1169724/1‑150 (MQ=255)                                         tcCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGcgcg                                                                                                          >  1:602402/1‑150 (MQ=255)                                               atcat‑cGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTgcgc                                                                                                    <  2:588720/150‑1 (MQ=255)                                                tcat‑cGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGtt                                                                                                                                  >  2:81001/1‑119 (MQ=255)                                                tcat‑cGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGtt                                                                                                                                  <  1:81001/119‑1 (MQ=255)                                                  at‑cGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTa                                                                                                 >  2:1025761/1‑150 (MQ=255)                                                   t‑cGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGGTTTTGTGTGCGCCAGGCATGGCGCGCTGCGCGTAt                                                                                                >  1:975236/1‑150 (MQ=255)                                                           cTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCATACGCGCAAc                                                                                         <  2:254860/150‑1 (MQ=255)                                                           cTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCATACGCGCAAc                                                                                         <  2:687482/150‑1 (MQ=255)                                                            tGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGG‑CCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCATACGCGCAAcgc                                                                                       <  1:1111155/150‑3 (MQ=39)                                                                  ccGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATCACACGGTGCGCGTATGCGCAACCCGCTTg                                                                                  >  1:907115/1‑150 (MQ=255)                                                                   cgatcgatAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAAccaagcggg                                                                                 <  2:674418/150‑8 (MQ=255)                                                                   cgatcgatAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGCCATGTGCATGACCgggg                                                                                                                                     >  1:750627/1‑98 (MQ=38)                                                                   cgatcgatAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGCCATGTGCATGACCgggg                                                                                                                                     <  2:750627/98‑1 (MQ=38)                                                                           aaaCTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTgtggtg                                                                         <  1:1084295/150‑1 (MQ=255)                                                                                  ctgagcagcagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGt                                                                  >  1:1205408/4‑150 (MQ=255)                                                                                   tCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGCCGCGTACGGCGTGATCCAAGGCCACCGGTCATTCACCTCGCGAGCAGCAGCTCGCAGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGATGGTGGTGGCCAAGTg                                                                 >  1:875669/1‑150 (MQ=12)                                                                                   tCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGTCAGGCATGGCGCGTTGCGCGTAt                                                                                                >  1:794454/1‑119 (MQ=255)                                                                                   tCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGTCAGGCATGGCGCGTTGCGCGTAt                                                                                                <  2:794454/119‑1 (MQ=255)                                                                                    cagcagcagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTgg                                                                <  2:1067173/150‑1 (MQ=255)                                                                                      gcagcagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                              >  1:646780/1‑150 (MQ=255)                                                                                      gcagcagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                              >  1:137969/1‑150 (MQ=255)                                                                                       cagcagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTgcgc                                                                                                    <  1:646882/111‑1 (MQ=255)                                                                                       cagcagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTgcgc                                                                                                    >  2:646882/1‑111 (MQ=255)                                                                                          cagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTtgt                                                                                                                             >  1:651616/1‑83 (MQ=255)                                                                                          cagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTtgt                                                                                                                             <  2:651616/83‑1 (MQ=255)                                                                                            gTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGcgcg                                                                                                          <  2:923828/100‑1 (MQ=255)                                                                                            gTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGcgcg                                                                                                          >  1:923828/1‑100 (MQ=255)                                                                                            gTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTg                                                        <  2:925834/150‑1 (MQ=255)                                                                                              aaTCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGtttt                                                                                                                                >  1:34637/1‑76 (MQ=255)                                                                                              aaTCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGtttt                                                                                                                                <  2:34637/76‑1 (MQ=255)                                                                                               aTCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAg                                                     >  2:428239/1‑150 (MQ=255)                                                                                                  ttGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTg                                                  >  1:277528/1‑150 (MQ=255)                                                                                                       ggTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTGGGCTGTGGCGTCCAAGTGGGGGACTTGGAGGTGAAAGt                                             >  2:1103472/1‑150 (MQ=25)                                                                                                         tACCCTGACGGCGACGCTTGCGGCGTGACGCAAGGCGCGCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTcc                                           <  2:975236/150‑1 (MQ=18)                                                                                                            ccTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                               >  2:1127908/1‑143 (MQ=255)                                                                                                            ccTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                               <  1:1127908/143‑1 (MQ=255)                                                                                                             cTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg                                                                            >  2:958443/1‑113 (MQ=255)                                                                                                             cTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg                                                                            <  1:958443/113‑1 (MQ=255)                                                                                                              tGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTac                                      >  2:477396/1‑150 (MQ=255)                                                                                                               gATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTaca                                     <  1:355797/150‑1 (MQ=255)                                                                                                                   gcgACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                              >  2:283538/1‑121 (MQ=255)                                                                                                                   gcgACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                              <  1:283538/121‑1 (MQ=255)                                                                                                                     gACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCg                               <  1:428239/150‑1 (MQ=255)                                                                                                                          ttGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAg                          <  2:1205408/150‑1 (MQ=255)                                                                                                                            gcggcgTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAggg                        <  1:1169724/150‑1 (MQ=255)                                                                                                                               gcgTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGCGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGaa                     <  2:875669/150‑1 (MQ=255)                                                                                                                                cgTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTGTGGGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAg                    <  1:385026/150‑1 (MQ=255)                                                                                                                                     tCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTacacac                                  >  2:329740/1‑131 (MQ=255)                                                                                                                                     tCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTacacac                                  <  1:329740/131‑1 (MQ=255)                                                                                                                                     tCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAgg                         >  2:342062/1‑140 (MQ=255)                                                                                                                                     tCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAgg                         <  1:342062/140‑1 (MQ=255)                                                                                                                                       cAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGc             >  2:64928/1‑150 (MQ=255)                                                                                                                                           gCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGa         >  2:338963/1‑150 (MQ=255)                                                                                                                                            cccccGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAg        <  1:477396/150‑1 (MQ=255)                                                                                                                                             ccccGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTa                                       >  1:608767/1‑118 (MQ=255)                                                                                                                                             ccccGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTa                                       <  2:608767/118‑1 (MQ=255)                                                                                                                                             ccccGGCCATGTGCATGACCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTgg                                                       >  2:776901/1‑102 (MQ=14)                                                                                                                                             ccccGGCCATGTGCATGACCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTgg                                                       <  1:776901/102‑1 (MQ=14)                                                                                                                                                cGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACCCCCCCGACAACGGGATGGGTTAGCCGGattcaa    >  2:861661/1‑145 (MQ=14)                                                                                                                                                  gTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGACAAGTGGGTGACTTGCAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAgg  <  2:167986/150‑1 (MQ=255)                                                                                                                                                   tCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAgg  <  1:519679/149‑1 (MQ=255)                                                                                                                                                   tCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAgg  >  2:519679/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CTGCACCTACGACTACCTGTTCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGG  >  NC_002947/1425512‑1425805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑