Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,425,662	C→T	6.9%	intergenic (+57/‑448)	PP_1249 → / → PP_1250	DUF4223 domain‑containing protein/group II intron‑encoding maturase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,425,662	0	C	T	6.9%	210.5 / 3.6	72	intergenic (+57/‑448)	PP_1249/PP_1250	DUF4223 domain‑containing protein/group II intron‑encoding maturase
Reads supporting (aligned to +/- strand):  ref base C (35/32);  new base T (2/3);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTACGACTACCTGTTCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGGTG  >  NC_002947/1425517‑1425808                                                                                                                                                   |                                                                                                                                                   ccTCCGACTACCTGTTCCACCCTTCGGTTTCCATCTCTAAGTTCAT‑CGGTGGCTGCTGCCCGTTCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGAACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACAt                                                                                                                                                <  1:314029/150‑1 (MQ=255)        ctcccTGTCCCACCCTTCGGTTTCCATCTCCAAGACAATACGGT‑GCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGCCATGTGCATGACCggg                                                                                                                                         <  1:919127/147‑1 (MQ=255)         tACCTGTTCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCgggg                                                                                                                                        >  2:1084295/1‑150 (MQ=255)            cTGTTCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGtt                                                                                                                                     >  2:355797/1‑150 (MQ=255)             tGTTCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGG‑CCCCGGTCATGCACATGGCCGGGGGtttt                                                                                                                                   >  2:1111155/1‑150 (MQ=255)               ttCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGttttt                                                                                                                                  >  1:925834/1‑150 (MQ=255)                                 aTCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGgc                                                                                                                <  1:944897/150‑1 (MQ=255)                                  tctcCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGgcg                                                                                                               >  1:674418/1‑150 (MQ=255)                                    tcCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGcgcg                                                                                                             >  1:602402/1‑150 (MQ=255)                                    tcCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGcgcg                                                                                                             >  2:1169724/1‑150 (MQ=255)                                          atcat‑cGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTgcgc                                                                                                       <  2:588720/150‑1 (MQ=255)                                           tcat‑cGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGtt                                                                                                                                     >  2:81001/1‑119 (MQ=255)                                           tcat‑cGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGtt                                                                                                                                     <  1:81001/119‑1 (MQ=255)                                             at‑cGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTa                                                                                                    >  2:1025761/1‑150 (MQ=255)                                              t‑cGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGGTTTTGTGTGCGCCAGGCATGGCGCGCTGCGCGTAt                                                                                                   >  1:975236/1‑150 (MQ=255)                                                      cTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCATACGCGCAAc                                                                                            <  2:254860/150‑1 (MQ=255)                                                      cTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCATACGCGCAAc                                                                                            <  2:687482/150‑1 (MQ=255)                                                       tGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGG‑CCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCATACGCGCAAcgc                                                                                          <  1:1111155/150‑3 (MQ=39)                                                             ccGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATCACACGGTGCGCGTATGCGCAACCCGCTTg                                                                                     >  1:907115/1‑150 (MQ=255)                                                              cgatcgatAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAAccaagcggg                                                                                    <  2:674418/150‑8 (MQ=255)                                                              cgatcgatAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGCCATGTGCATGACCgggg                                                                                                                                        <  2:750627/98‑1 (MQ=38)                                                              cgatcgatAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGCCATGTGCATGACCgggg                                                                                                                                        >  1:750627/1‑98 (MQ=38)                                                                      aaaCTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTgtggtg                                                                            <  1:1084295/150‑1 (MQ=255)                                                                             ctgagcagcagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGt                                                                     >  1:1205408/4‑150 (MQ=255)                                                                              tCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGCCGCGTACGGCGTGATCCAAGGCCACCGGTCATTCACCTCGCGAGCAGCAGCTCGCAGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGATGGTGGTGGCCAAGTg                                                                    >  1:875669/1‑150 (MQ=12)                                                                              tCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGTCAGGCATGGCGCGTTGCGCGTAt                                                                                                   <  2:794454/119‑1 (MQ=255)                                                                              tCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGTCAGGCATGGCGCGTTGCGCGTAt                                                                                                   >  1:794454/1‑119 (MQ=255)                                                                               cagcagcagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTgg                                                                   <  2:1067173/150‑1 (MQ=255)                                                                                 gcagcagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                                 >  1:646780/1‑150 (MQ=255)                                                                                 gcagcagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                                 >  1:137969/1‑150 (MQ=255)                                                                                  cagcagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTgcgc                                                                                                       <  1:646882/111‑1 (MQ=255)                                                                                  cagcagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTgcgc                                                                                                       >  2:646882/1‑111 (MQ=255)                                                                                     cagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTtgt                                                                                                                                >  1:651616/1‑83 (MQ=255)                                                                                     cagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTtgt                                                                                                                                <  2:651616/83‑1 (MQ=255)                                                                                       gTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGcgcg                                                                                                             >  1:923828/1‑100 (MQ=255)                                                                                       gTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGcgcg                                                                                                             <  2:923828/100‑1 (MQ=255)                                                                                       gTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTg                                                           <  2:925834/150‑1 (MQ=255)                                                                                         aaTCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGtttt                                                                                                                                   <  2:34637/76‑1 (MQ=255)                                                                                         aaTCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGtttt                                                                                                                                   >  1:34637/1‑76 (MQ=255)                                                                                          aTCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAg                                                        >  2:428239/1‑150 (MQ=255)                                                                                             ttGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTg                                                     >  1:277528/1‑150 (MQ=255)                                                                                                  ggTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTGGGCTGTGGCGTCCAAGTGGGGGACTTGGAGGTGAAAGt                                                >  2:1103472/1‑150 (MQ=25)                                                                                                    tACCCTGACGGCGACGCTTGCGGCGTGACGCAAGGCGCGCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTcc                                              <  2:975236/150‑1 (MQ=18)                                                                                                       ccTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                                  <  1:1127908/143‑1 (MQ=255)                                                                                                       ccTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                                  >  2:1127908/1‑143 (MQ=255)                                                                                                        cTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg                                                                               >  2:958443/1‑113 (MQ=255)                                                                                                        cTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg                                                                               <  1:958443/113‑1 (MQ=255)                                                                                                         tGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTac                                         >  2:477396/1‑150 (MQ=255)                                                                                                          gATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTaca                                        <  1:355797/150‑1 (MQ=255)                                                                                                              gcgACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                                 >  2:283538/1‑121 (MQ=255)                                                                                                              gcgACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                                 <  1:283538/121‑1 (MQ=255)                                                                                                                gACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCg                                  <  1:428239/150‑1 (MQ=255)                                                                                                                     ttGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAg                             <  2:1205408/150‑1 (MQ=255)                                                                                                                       gcggcgTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAggg                           <  1:1169724/150‑1 (MQ=255)                                                                                                                          gcgTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGCGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGaa                        <  2:875669/150‑1 (MQ=255)                                                                                                                           cgTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTGTGGGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAg                       <  1:385026/150‑1 (MQ=255)                                                                                                                                tCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTacacac                                     <  1:329740/131‑1 (MQ=255)                                                                                                                                tCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTacacac                                     >  2:329740/1‑131 (MQ=255)                                                                                                                                tCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAgg                            <  1:342062/140‑1 (MQ=255)                                                                                                                                tCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAgg                            >  2:342062/1‑140 (MQ=255)                                                                                                                                  cAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGc                >  2:64928/1‑150 (MQ=255)                                                                                                                                      gCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGa            >  2:338963/1‑150 (MQ=255)                                                                                                                                       cccccGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAg           <  1:477396/150‑1 (MQ=255)                                                                                                                                        ccccGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTa                                          <  2:608767/118‑1 (MQ=255)                                                                                                                                        ccccGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTa                                          >  1:608767/1‑118 (MQ=255)                                                                                                                                        ccccGGCCATGTGCATGACCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTgg                                                          >  2:776901/1‑102 (MQ=14)                                                                                                                                        ccccGGCCATGTGCATGACCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTgg                                                          <  1:776901/102‑1 (MQ=14)                                                                                                                                           cGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACCCCCCCGACAACGGGATGGGTTAGCCGGattcaa       >  2:861661/1‑145 (MQ=14)                                                                                                                                             gTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGACAAGTGGGTGACTTGCAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAgg     <  2:167986/150‑1 (MQ=255)                                                                                                                                              tCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAgg     <  1:519679/149‑1 (MQ=255)                                                                                                                                              tCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAgg     >  2:519679/1‑149 (MQ=255)                                                                                                                                                aTGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGgtg  >  2:535439/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CCTACGACTACCTGTTCCACCCTTCGGTTTCCATCTCCAAGATCAT‑CGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCGGGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGGTG  >  NC_002947/1425517‑1425808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑