Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,435,218 T→A 7.7% Q251H (CAA→CAT)  PP_1256 ← alpha‑ketoglutarate semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,435,2180TA 7.7% 148.9 / 3.0 52Q251H (CAA→CAT) PP_1256alpha‑ketoglutarate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (25/23);  new base A (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

AACTGGCCGGCGCCCTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGCACTGCTTC  >  NC_002947/1435069‑1435359
                                                                                                                                                     |                                                                                                                                             
aaCTGGCCGGCGCCCTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCt                                                                                                                                               <  1:737923/150‑1 (MQ=255)
  cTGGCCGGCGCCCTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTg                                                                                                                                             <  2:958393/150‑1 (MQ=255)
     gCCGGCGCCCTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGca                                                                                                                                          <  2:213694/150‑1 (MQ=255)
              cTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGtgccat                                                                                                                                 <  1:441026/150‑1 (MQ=255)
                           gCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTgcgc                                                                                                                    >  1:307900/1‑150 (MQ=255)
                                gaAACCTTGTGCCAGTTCTTCGGCACGGGCTTGCAGGGCGGCGTCGCACAGGAACACCGGGTTGATCGAACTCATTTCGGCATCCACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGcc                                                                                                               >  2:141054/1‑150 (MQ=255)
                                 aaaCCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCg                                                                                                              >  1:768648/1‑150 (MQ=255)
                                      ttGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGaa                                                                                                         <  2:1143224/150‑1 (MQ=255)
                                       tgggCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGaaa                                                                                                        <  1:43012/147‑1 (MQ=255)
                                          gCCAGTGCGTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATAGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCCCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTTCTCCAGTCGGTGAAACCa                                                                                                     >  2:63223/1‑150 (MQ=255)
                                              gTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACgg                                                                                                 <  1:323513/150‑1 (MQ=255)
                                                  ttCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCtt                                                                                             >  2:117651/1‑150 (MQ=255)
                                                      gCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGtgcc                                                                                                                                   >  1:157241/1‑108 (MQ=255)
                                                      gCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGtgcc                                                                                                                                   <  2:157241/108‑1 (MQ=255)
                                                      gCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATa                                                                                         >  2:274270/1‑150 (MQ=255)
                                                             cTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGt                                                                                  <  1:274270/150‑1 (MQ=255)
                                                                 cAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCt                                                                              >  1:531702/1‑150 (MQ=255)
                                                                    ggcggcGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGAc                                                                           <  2:307900/150‑1 (MQ=255)
                                                                         cgTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCagag                                                                      <  1:386521/150‑1 (MQ=255)
                                                                               aCAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCAGGGCGGCTTGGCACAGTGCCATAGCGCCGCTGCGGGAGCGTGTGTAACCAACGGCGTTTATATGGGGGTCGCTGACGAGGGGAATgg                                                                >  2:553845/1‑149 (MQ=255)
                                                                                cAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGcc                                                               <  1:117651/150‑1 (MQ=255)
                                                                                 aGGAACACCGGGTTGATCGATCTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCa                                                                         <  1:43735/139‑1 (MQ=255)
                                                                                 aGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAGCGGCCTTGATACGTGGGTCGCTGACCa                                                                         >  2:43735/1‑139 (MQ=255)
                                                                                   gAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATg                                                                 >  1:552487/1‑145 (MQ=255)
                                                                                   gAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATg                                                                 <  2:552487/145‑1 (MQ=255)
                                                                                           ggTTGATCGAACTCATTTCGGCATACAGCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTccacgt                                                    <  1:947932/150‑6 (MQ=255)
                                                                                                aTCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGcc                                                               <  1:922233/134‑1 (MQ=255)
                                                                                                aTCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGcc                                                               >  2:922233/1‑134 (MQ=255)
                                                                                                  cGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTgg                                                                                    >  2:166924/1‑111 (MQ=255)
                                                                                                  cGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTgg                                                                                    <  1:166924/111‑1 (MQ=255)
                                                                                                       tCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCATGGCACTGtgccaagcc                                                                                                                              <  1:1153331/64‑1 (MQ=38)
                                                                                                       tCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCATGGCACTGtgccaagcc                                                                                                                              >  2:1153331/1‑64 (MQ=37)
                                                                                                          tttCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCaga                                                                       >  1:640748/1‑116 (MQ=255)
                                                                                                          tttCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCaga                                                                       <  2:640748/116‑1 (MQ=255)
                                                                                                                 aTACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGaa                              <  2:411196/150‑1 (MQ=255)
                                                                                                                 aTACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGaa                              <  2:768648/150‑1 (MQ=255)
                                                                                                                    cacCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAacac                           >  2:1192559/1‑150 (MQ=255)
                                                                                                                     acCGGGATGGGCTCCGGGCGCGCCTGGGCGTCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAacaca                          <  1:141054/150‑1 (MQ=255)
                                                                                                                     acCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGATCCGGTGAAACCAACGGCCTTGATACGTGGGTCGTTGACCAGAGCAATGCCCACTTCGCGGTCGGAGCCATAATGCAGCGAGAacaca                          >  2:491029/1‑150 (MQ=255)
                                                                                                                     acCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAacaca                          >  2:490488/1‑150 (MQ=255)
                                                                                                                             gggCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCgg                  <  1:490488/150‑1 (MQ=255)
                                                                                                                                 tCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAg              >  1:484146/1‑150 (MQ=255)
                                                                                                                                        gcgcCTGGGCGGCTTGGCACAGTGCCATGTCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGa                                                                            >  1:552139/1‑81 (MQ=255)
                                                                                                                                        gcgcCTGGGCGGCTTGGCACAGTGCCATGTCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGa                                                                            <  2:552139/81‑1 (MQ=255)
                                                                                                                                          gcCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAacacac                         <  2:1094811/130‑1 (MQ=255)
                                                                                                                                          gcCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAacacac                         >  1:1094811/1‑130 (MQ=255)
                                                                                                                                          gcCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGcactgc     >  1:212300/1‑150 (MQ=255)
                                                                                                                                          gcCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGcactgc     >  1:730676/1‑150 (MQ=255)
                                                                                                                                          gcCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCAACTTCCCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGcactgc     >  2:822970/1‑150 (MQ=255)
                                                                                                                                           ccTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGACACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCACGGCCCGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAACCCGcactgct    >  2:525209/1‑150 (MQ=255)
                                                                                                                                             tGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGcactgcttc  >  1:420394/1‑150 (MQ=255)
                                                                                                                                                gtggcATGGCACTGTGCCAAGCCGCCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGa                                                >  1:381293/3‑101 (MQ=38)
                                                                                                                                                gtggcATGGCACTGTGCCAAGCCGCCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGa                                                <  2:381293/99‑1 (MQ=38)
                                                                                                                                                     |                                                                                                                                             
AACTGGCCGGCGCCCTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGCACTGCTTC  >  NC_002947/1435069‑1435359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑