Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,435,225 A→T 10.8% L249Q (CTG→CAG)  PP_1256 ← alpha‑ketoglutarate semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,435,2250AT10.8% 156.1 / 8.9 55L249Q (CTG→CAG) PP_1256alpha‑ketoglutarate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (27/22);  new base T (3/3);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

CTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGCACTGCTTCACTGCTTGCGCCA  >  NC_002947/1435083‑1435372
                                                                                                                                              |                                                                                                                                                   
cTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGtgccat                                                                                                                                              <  1:441026/150‑1 (MQ=255)
             gCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTgcgc                                                                                                                                 >  1:307900/1‑150 (MQ=255)
                  gaAACCTTGTGCCAGTTCTTCGGCACGGGCTTGCAGGGCGGCGTCGCACAGGAACACCGGGTTGATCGAACTCATTTCGGCATCCACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGcc                                                                                                                            >  2:141054/1‑150 (MQ=255)
                   aaaCCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCg                                                                                                                           >  1:768648/1‑150 (MQ=255)
                        ttGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGaa                                                                                                                      <  2:1143224/150‑1 (MQ=255)
                         tgggCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGaaa                                                                                                                     <  1:43012/147‑1 (MQ=255)
                            gCCAGTGCGTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATAGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCCCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTTCTCCAGTCGGTGAAACCa                                                                                                                  >  2:63223/1‑150 (MQ=255)
                                gTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACgg                                                                                                              <  1:323513/150‑1 (MQ=255)
                                    ttCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCtt                                                                                                          >  2:117651/1‑150 (MQ=255)
                                        gCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGtgcc                                                                                                                                                >  1:157241/1‑108 (MQ=255)
                                        gCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGtgcc                                                                                                                                                <  2:157241/108‑1 (MQ=255)
                                        gCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATa                                                                                                      >  2:274270/1‑150 (MQ=255)
                                               cTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGt                                                                                               <  1:274270/150‑1 (MQ=255)
                                                   cAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCt                                                                                           >  1:531702/1‑150 (MQ=255)
                                                      ggcggcGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGAc                                                                                        <  2:307900/150‑1 (MQ=255)
                                                           cgTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCagag                                                                                   <  1:386521/150‑1 (MQ=255)
                                                                 aCAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCAGGGCGGCTTGGCACAGTGCCATAGCGCCGCTGCGGGAGCGTGTGTAACCAACGGCGTTTATATGGGGGTCGCTGACGAGGGGAATgg                                                                             >  2:553845/1‑149 (MQ=255)
                                                                  cAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGcc                                                                            <  1:117651/150‑1 (MQ=255)
                                                                   aGGAACACCGGGTTGATCGATCTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCa                                                                                      <  1:43735/139‑1 (MQ=255)
                                                                   aGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAGCGGCCTTGATACGTGGGTCGCTGACCa                                                                                      >  2:43735/1‑139 (MQ=255)
                                                                     gAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATg                                                                              >  1:552487/1‑145 (MQ=255)
                                                                     gAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATg                                                                              <  2:552487/145‑1 (MQ=255)
                                                                             ggTTGATCGAACTCATTTCGGCATACAGCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTccacgt                                                                 <  1:947932/150‑6 (MQ=255)
                                                                                  aTCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGcc                                                                            >  2:922233/1‑134 (MQ=255)
                                                                                  aTCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGcc                                                                            <  1:922233/134‑1 (MQ=255)
                                                                                    cGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTgg                                                                                                 >  2:166924/1‑111 (MQ=255)
                                                                                    cGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTgg                                                                                                 <  1:166924/111‑1 (MQ=255)
                                                                                         tCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCATGGCACTGtgccaagcc                                                                                                                                           <  1:1153331/64‑1 (MQ=38)
                                                                                         tCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCATGGCACTGtgccaagcc                                                                                                                                           >  2:1153331/1‑64 (MQ=37)
                                                                                            tttCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCaga                                                                                    >  1:640748/1‑116 (MQ=255)
                                                                                            tttCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCaga                                                                                    <  2:640748/116‑1 (MQ=255)
                                                                                                   aTACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGaa                                           <  2:768648/150‑1 (MQ=255)
                                                                                                   aTACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGaa                                           <  2:411196/150‑1 (MQ=255)
                                                                                                      cacCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAacac                                        >  2:1192559/1‑150 (MQ=255)
                                                                                                       acCGGGATGGGCTCCGGGCGCGCCTGGGCGTCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAacaca                                       <  1:141054/150‑1 (MQ=255)
                                                                                                       acCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGATCCGGTGAAACCAACGGCCTTGATACGTGGGTCGTTGACCAGAGCAATGCCCACTTCGCGGTCGGAGCCATAATGCAGCGAGAacaca                                       >  2:491029/1‑150 (MQ=255)
                                                                                                       acCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAacaca                                       >  2:490488/1‑150 (MQ=255)
                                                                                                               gggCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCgg                               <  1:490488/150‑1 (MQ=255)
                                                                                                                   tCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAg                           >  1:484146/1‑150 (MQ=255)
                                                                                                                          gcgcCTGGGCGGCTTGGCACAGTGCCATGTCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGa                                                                                         <  2:552139/81‑1 (MQ=255)
                                                                                                                          gcgcCTGGGCGGCTTGGCACAGTGCCATGTCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGa                                                                                         >  1:552139/1‑81 (MQ=255)
                                                                                                                            gcCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAacacac                                      >  1:1094811/1‑130 (MQ=255)
                                                                                                                            gcCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAacacac                                      <  2:1094811/130‑1 (MQ=255)
                                                                                                                            gcCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGcactgc                  >  1:212300/1‑150 (MQ=255)
                                                                                                                            gcCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGcactgc                  >  1:730676/1‑150 (MQ=255)
                                                                                                                            gcCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCAACTTCCCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGcactgc                  >  2:822970/1‑150 (MQ=255)
                                                                                                                             ccTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGACACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCACGGCCCGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAACCCGcactgct                 >  2:525209/1‑150 (MQ=255)
                                                                                                                               tGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGcactgcttc               >  1:420394/1‑150 (MQ=255)
                                                                                                                                  gtggcatGGCACTGTGCCAAGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGcactgcttcact            >  1:992814/7‑150 (MQ=255)
                                                                                                                                  gtggcATGGCACTGTGCCAAGCCGCCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGa                                                             <  2:381293/99‑1 (MQ=38)
                                                                                                                                  gtggcATGGCACTGTGCCAAGCCGCCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGa                                                             >  1:381293/3‑101 (MQ=38)
                                                                                                                                      catGGCACTGTGCCAAGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGcactgcttcactgctt        <  2:992814/148‑1 (MQ=255)
                                                                                                                                         ggCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGCACTGCTTCACTGCTTgcg     <  2:484146/150‑1 (MQ=255)
                                                                                                                                         ggCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGCACTGCTTCACTGCTTgcg     >  2:1068406/1‑150 (MQ=255)
                                                                                                                                         ggCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGCACTGCTTCACTGCTTgcg     >  1:693120/1‑150 (MQ=255)
                                                                                                                                            acaGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGCACTGCTTCACTGCTTGCGCCa  >  1:460259/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGCACTGCTTCACTGCTTGCGCCA  >  NC_002947/1435083‑1435372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑