Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,435,829 A→T 15.1% C48S (TGC→AGC)  PP_1256 ← alpha‑ketoglutarate semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,435,8290AT15.1% 140.2 / 14.3 52C48S (TGC→AGC) PP_1256alpha‑ketoglutarate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (22/22);  new base T (4/4);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTC  >  NC_002947/1435682‑1435978
                                                                                                                                                   |                                                                                                                                                      
ccTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAgg                                                                                                                                                      >  2:628231/1‑150 (MQ=255)
     ggCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTgg                                                                                                                                                 <  1:1014773/150‑1 (MQ=255)
     ggCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTgg                                                                                                                                                 <  1:1071108/150‑1 (MQ=255)
     ggCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGAGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTgg                                                                                                                                                 <  2:800528/150‑1 (MQ=255)
     ggCAGGCCGGCTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGACTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTgg                                                                                                                                                 <  1:707227/150‑1 (MQ=255)
      gCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCTGCGCGCTGGCCTggg                                                                                                                                                <  1:723614/150‑1 (MQ=255)
      gCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTggg                                                                                                                                                <  2:547358/150‑1 (MQ=255)
          gCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCAc                                                                                                                                            <  2:1127979/150‑1 (MQ=255)
          gCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCAc                                                                                                                                            <  1:1128553/150‑1 (MQ=255)
                ttCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGTCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTc                                                                                                                                      <  2:186497/150‑1 (MQ=255)
                ttCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTc                                                                                                                                      >  2:490971/1‑150 (MQ=255)
                                 cGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGt                                                                                                                     >  2:503320/1‑150 (MQ=255)
                                               ccGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAAc                                                                                                       <  1:241763/150‑1 (MQ=255)
                                                       cTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGAcc                                                                                               >  1:997035/1‑150 (MQ=255)
                                                       cTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGAcc                                                                                               >  2:397477/1‑150 (MQ=255)
                                                       cTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGAcc                                                                                               >  2:917036/1‑150 (MQ=255)
                                                            tCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGccgccg                                                                                                                         <  1:610099/119‑1 (MQ=255)
                                                            tCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGccgccg                                                                                                                         >  2:610099/1‑119 (MQ=255)
                                                                tgtgGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCCCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑Tgg                                                                                     <  2:807619/150‑1 (MQ=255)
                                                                  tgGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑Tgggg                                                                                   >  2:1049779/1‑149 (MQ=255)
                                                                    gCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCg                                                                                 >  1:965438/1‑150 (MQ=255)
                                                                      gATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCTGCGCGCTGGCCTggg                                                                                                                                                >  1:1171305/1‑86 (MQ=255)
                                                                      gATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCTGCGCGCTGGCCTggg                                                                                                                                                <  2:1171305/86‑1 (MQ=255)
                                                                       aTGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATg                                                                              >  2:360467/1‑150 (MQ=255)
                                                                       aTGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCCCCAACGTCTGACCGGTGGT‑TGGGTCCATg                                                                              >  2:928558/1‑150 (MQ=255)
                                                                           cTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCAc                                                                          >  1:1059000/1‑150 (MQ=255)
                                                                            ttCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCTCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCAGAc                                                                                                <  1:977612/128‑1 (MQ=255)
                                                                            ttCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCAGAc                                                                                                >  2:977612/1‑128 (MQ=255)
                                                                              cAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGa                                                                       <  1:280499/150‑1 (MQ=255)
                                                                               aaGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGAt                                                                      >  2:418323/1‑150 (MQ=255)
                                                                                aGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAGCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATg                                                                     >  1:307520/1‑150 (MQ=255)
                                                                                      tctGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCt                                                                                                   <  2:890749/115‑1 (MQ=255)
                                                                                      tctGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCt                                                                                                   >  1:890749/1‑115 (MQ=255)
                                                                                           tCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCACCAACGTCTGACCGGTGGT‑TGGGCCGATGGCACGGATGGCGTCGCGGCt                                                          >  2:263292/1‑150 (MQ=255)
                                                                                               tgttCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCg                                                      <  1:917036/150‑1 (MQ=255)
                                                                                                      gCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTAGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCg                                               >  2:260671/1‑150 (MQ=255)
                                                                                                              tCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTgg                                       <  2:965438/150‑1 (MQ=255)
                                                                                                                   gTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGAt                                  <  1:206483/150‑1 (MQ=255)
                                                                                                                         aTCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGTTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagcag                            <  1:643859/150‑1 (MQ=255)
                                                                                                                            gAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGtt                         <  2:1059000/150‑1 (MQ=255)
                                                                                                                                 ccgccCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagcag                            <  2:761717/142‑1 (MQ=255)
                                                                                                                                 ccgccCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagcag                            >  1:761717/1‑142 (MQ=255)
                                                                                                                                  cgccCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTTTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCtgt                   <  1:1102736/150‑1 (MQ=255)
                                                                                                                                    cccAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGa                 >  2:953728/1‑150 (MQ=255)
                                                                                                                                     ccAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGc                                             >  1:282120/1‑121 (MQ=255)
                                                                                                                                     ccAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGc                                             <  2:282120/121‑1 (MQ=255)
                                                                                                                                     ccAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAg                >  1:15186/1‑150 (MQ=255)
                                                                                                                                       aGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGc                                                                   >  2:743597/1‑97 (MQ=255)
                                                                                                                                       aGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGc                                                                   <  1:743597/97‑1 (MQ=255)
                                                                                                                                         gCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGc            >  1:761588/1‑150 (MQ=255)
                                                                                                                                          ccAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCa           >  2:835448/1‑150 (MQ=255)
                                                                                                                                           cAGCGCGCAGGTCTGGGCCCCGAGTCCGGGTTGCCCTCCGATTTAGGCCGGCTCCAACGT‑AGAAGCGTGCTCTTGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCAt          <  1:928558/150‑1 (MQ=255)
                                                                                                                                                   aGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGcctc  <  1:260671/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
CCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGT‑TGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTC  >  NC_002947/1435682‑1435978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑