Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,443,379 C→A 100% A272S (GCC→TCC)  PP_1262 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,443,3790CA100.0% 132.7 / NA 41A272S (GCC→TCC) PP_1262LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/19);  total (22/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATCGGCGGTGTTCA  >  NC_002947/1443238‑1443525
                                                                                                                                             |                                                                                                                                                  
cagcTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGt                                                                                                                                            >  1:544802/1‑150 (MQ=255)
    tttttAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCaa                                                                                                                                        <  1:1077825/150‑1 (MQ=255)
          gTTGGCGATCAGCACCTGATGGGTTTTCAGGACTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTg                                                                                                                                  >  2:743896/1‑150 (MQ=255)
            tGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCa                                                                                                                                <  2:557245/150‑1 (MQ=255)
                    aGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATAc                                                                                                                        <  2:1123240/150‑1 (MQ=255)
                        ccTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCg                                                                                                                    <  2:432036/150‑1 (MQ=255)
                           gATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCa                                                                                                                 <  2:613303/150‑1 (MQ=255)
                               ggTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTAc                                                                                                             >  2:1081212/1‑150 (MQ=255)
                                 ttttCAGGTCTGCCTCATGCGCGGCCAGAATCTGTGGCAGCGAGTTGCGCATGTACTCGACCCACGACTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGTTTCAACTCCTACAGGCGAGACTTCGTCAGTTccc                                                                                                           >  2:70304/1‑150 (MQ=255)
                                 ttttCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGt                                                                                                                                            <  2:1028774/117‑1 (MQ=255)
                                 ttttCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGt                                                                                                                                            >  1:1028774/1‑117 (MQ=255)
                                             ccTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTAc                                                                                               >  2:729765/1‑150 (MQ=255)
                                                   gcgcGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCCACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTccc                                                                                         <  1:560595/150‑1 (MQ=255)
                                                               tctcTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGAATAGATGGAATACAg                                                                                                                                              <  2:98776/85‑1 (MQ=255)
                                                               tctcTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGAATAGATGGAATACAg                                                                                                                                              >  1:98776/1‑85 (MQ=255)
                                                                                 gcAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGaa                                                                     >  2:491482/1‑140 (MQ=255)
                                                                                 gcAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGaa                                                                     <  1:491482/140‑1 (MQ=255)
                                                                                  cAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAgg                                                                                                                                             <  2:341528/67‑1 (MQ=255)
                                                                                  cAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAgg                                                                                                                                             >  1:341528/1‑67 (MQ=255)
                                                                                        cTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGcc                                                    >  2:157307/1‑150 (MQ=255)
                                                                                                aGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGc                                                                         >  1:149144/1‑121 (MQ=255)
                                                                                                aGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGc                                                                         <  2:149144/121‑1 (MQ=255)
                                                                                                aGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCa                                            >  2:454254/1‑150 (MQ=255)
                                                                                                    cTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGcc                                        >  1:420175/1‑150 (MQ=255)
                                                                                                     ttGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCa                                       >  1:795682/1‑150 (MQ=255)
                                                                                                     ttGATATTGGCATAGAGATACTGCAGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGCCCCGCAGGCCCTCAATAGCCGCATAGATCGGCAGCACGCCTACGCCCATGCCa                                       <  2:544802/150‑1 (MQ=255)
                                                                                                       gATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATGGATGGGCAGCACGCCTACGCCCATGCCACt                                     >  1:306881/1‑150 (MQ=255)
                                                                                                        aTCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGaa                                                                     >  1:423215/1‑117 (MQ=255)
                                                                                                        aTCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGaa                                                                     <  2:423215/117‑1 (MQ=255)
                                                                                                                aTCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGc                            >  2:1100439/1‑150 (MQ=255)
                                                                                                                 tCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCa                           <  1:1081212/150‑1 (MQ=255)
                                                                                                                   cAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGAt                               <  1:751755/144‑1 (MQ=255)
                                                                                                                   cAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGAt                               >  2:751755/1‑144 (MQ=255)
                                                                                                                       tACTGCCGCGATGGATAGATGGAAACCAGGTGCTCCCCCTTGAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTc                     <  1:983915/150‑1 (MQ=255)
                                                                                                                            ccGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAAt                                                                    >  1:764221/1‑98 (MQ=255)
                                                                                                                            ccGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAAt                                                                    <  2:764221/98‑1 (MQ=255)
                                                                                                                                    gATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGcc                                                                                  <  2:1140067/76‑1 (MQ=255)
                                                                                                                                    gATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGcc                                                                                  >  1:1140067/1‑76 (MQ=255)
                                                                                                                                         aTGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATCGGCGGTGTTc   <  1:743896/150‑1 (MQ=255)
                                                                                                                                         aTGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATCGGCGGTGTTc   >  1:405376/1‑150 (MQ=255)
                                                                                                                                          tGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATCGGCGGTGTTCa  >  1:228620/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
CAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATCGGCGGTGTTCA  >  NC_002947/1443238‑1443525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑