Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,463,032 A→T 11.9% intergenic (‑101/+41) algI ← / ← algL alginate O‑acetylase AlgI/alginate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,463,0320AT11.9% 118.0 / 8.2 42intergenic (‑101/+41)algI/algLalginate O‑acetylase AlgI/alginate lyase
Reads supporting (aligned to +/- strand):  ref base A (21/16);  new base T (3/2);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01

GAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCACATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCGTGTCGCCAAC  >  NC_002947/1462884‑1463180
                                                                                                                                                    |                                                                                                                                                    
gAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAg                                                                                                                                                     >  1:114391/1‑150 (MQ=255)
     tCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGc                                                                                                                                                >  2:857404/1‑150 (MQ=255)
        gCAAGAACAGGAACAGGAAAACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCGcc                                                                                                                                             >  1:57074/1‑150 (MQ=255)
                aggaacaggaacACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAACGGGCAAGCCCGCTCCCACAGGtt                                                                                                                                     >  1:345175/1‑150 (MQ=255)
                                   aCGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGt                                                                                                                  >  1:388016/1‑150 (MQ=255)
                                      agaagaCCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCaa                                                                                                               <  1:857404/150‑1 (MQ=255)
                                          gaCCATCCCGGTTCTCCTAATTTGTTAACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCa                                                                                                           >  1:822464/1‑150 (MQ=255)
                                             cATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCaa                                                                                                               >  2:716668/1‑143 (MQ=255)
                                             cATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCaa                                                                                                               <  1:716668/143‑1 (MQ=255)
                                                cccGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCTTGCCCGCTCCCACAgg                                                                                                                                       >  2:880676/1‑116 (MQ=255)
                                                cccGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCTTGCCCGCTCCCACAgg                                                                                                                                       <  1:880676/116‑1 (MQ=255)
                                                        tcCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCtt                                                                                                     >  1:410691/1‑142 (MQ=255)
                                                        tcCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCtt                                                                                                     <  2:410691/142‑1 (MQ=255)
                                                        tcCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCtt                                                                                                     <  2:410593/142‑1 (MQ=255)
                                                        tcCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCtt                                                                                                     >  1:410593/1‑142 (MQ=255)
                                                                  ttCACGGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGctctct                                                                                              <  2:720178/139‑1 (MQ=255)
                                                                  ttCACGGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGctctct                                                                                              >  1:720178/1‑139 (MQ=255)
                                                                  ttCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTTGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTc                                                                                   <  1:669241/150‑1 (MQ=255)
                                                                  ttCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTc                                                                                   >  1:1097795/1‑150 (MQ=255)
                                                                     acaGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCgg                                                                                      <  2:627556/144‑1 (MQ=255)
                                                                     acaGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCgg                                                                                      >  1:627556/1‑144 (MQ=255)
                                                                          aTCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGAccc                                                                           <  1:780842/150‑1 (MQ=255)
                                                                              aGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGt                                                                       >  2:241958/1‑150 (MQ=255)
                                                                                ggCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGct                                                                                                  <  2:285292/121‑1 (MQ=255)
                                                                                ggCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGct                                                                                                  >  1:285292/1‑121 (MQ=255)
                                                                                            cccAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCACATCGCCGCCCa                                                         <  2:822464/150‑1 (MQ=255)
                                                                                             ccAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCAttt                                                                                                        <  1:428084/102‑1 (MQ=255)
                                                                                             ccAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCAttt                                                                                                        >  2:428084/1‑102 (MQ=255)
                                                                                               aaaCCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCACATCGCCGCCCAGcc                                                      <  2:388016/150‑1 (MQ=255)
                                                                                               aaaCCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTCCTCTCTGCACTCGGGTCGTAGACCCGCGTCACATCGCCGCCCAGcc                                                      >  1:1037136/1‑150 (MQ=255)
                                                                                                      ccAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCACATCGCCGCCCAGCCGGAAGCt                                               >  1:366488/1‑150 (MQ=255)
                                                                                                                    gAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTg                                                                                                    <  1:359555/83‑1 (MQ=255)
                                                                                                                    gAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTg                                                                                                    >  2:359555/1‑83 (MQ=255)
                                                                                                                        gCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCACATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTc                             <  2:366488/150‑1 (MQ=255)
                                                                                                                                   cccTGTGGGAGCGGGCAAGCGCGCTCCCACAGGTTTCGCCCCTGCCATGGGGGGCAATGAAATTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCACATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCt                  >  1:930158/1‑150 (MQ=255)
                                                                                                                                    ccTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGctctct                                                                                              >  2:33213/1‑73 (MQ=255)
                                                                                                                                    ccTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGctctct                                                                                              <  1:33213/73‑1 (MQ=255)
                                                                                                                                           gAGCGGGCTTGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCACATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCgtg          >  2:424782/1‑150 (MQ=255)
                                                                                                                                           gAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTa                                                                                <  1:1165142/80‑1 (MQ=255)
                                                                                                                                           gAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTa                                                                                >  2:1165142/1‑80 (MQ=255)
                                                                                                                                            aGCGGGCTTGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCACATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCgtgt         <  1:424782/150‑1 (MQ=255)
                                                                                                                                            aGCGGGCTTGCCCGCTACCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCACATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCgtgt         >  1:38862/1‑150 (MQ=255)
                                                                                                                                                   aaGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCACATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCGTGTCGCCAAc  >  2:716501/1‑150 (MQ=255)
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GAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTGGGAGCGGGCAAGCCCGCTCCCACAGGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGCACTCGGGTCGTAGACCCGCGTCACATCGCCGCCCAGCCGGAAGCTGTTGAACGGCTGCATGTCGTGCTTGCGCTCAAGCGTGTCGCCAAC  >  NC_002947/1462884‑1463180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑