Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,475,002 T→G 18.1% intergenic (‑775/+26) algD ← / ← PP_1289 GDP‑mannose 6‑dehydrogenase/DUF328 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,475,0020TG18.1% 82.9 / 16.2 44intergenic (‑775/+26)algD/PP_1289GDP‑mannose 6‑dehydrogenase/DUF328 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (15/21);  new base G (4/4);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

AAAAAAGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTG  >  NC_002947/1474856‑1475147
                                                                                                                                                  |                                                                                                                                                 
aaaaaaGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCTTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAg                                                                                                                                                >  1:206079/1‑150 (MQ=255)
aaaaaaGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAg                                                                                                                                                >  2:25262/1‑150 (MQ=255)
       gTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGCCCTCTTCGCGGCTGAAGCCGCGaa                                                                                                                                         <  2:161735/150‑1 (MQ=255)
            aaCGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAggg                                                                                                                                    >  1:745499/1‑150 (MQ=255)
              cGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGTCCTCTTCGCGGCTTCAGCCGCGAAGAGGGCg                                                                                                                                  <  2:741841/150‑1 (MQ=255)
                 tCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGa                                                                                                                               <  1:1190039/150‑1 (MQ=255)
                     aTGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAgg                                                                                                                           <  1:82343/150‑1 (MQ=255)
                      tGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTGAAGCCGCGAagag                                                                                                                                      >  1:245437/1‑138 (MQ=255)
                      tGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTGAAGCCGCGAagag                                                                                                                                      <  2:245437/138‑1 (MQ=255)
                                  aTAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCggg                                                                                                              <  2:288482/150‑1 (MQ=255)
                                      aGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGa                                                                                                          >  1:845190/1‑150 (MQ=255)
                                       gggAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACCCCGCGGAGTGag                                                                                                         <  1:25262/150‑2 (MQ=255)
                                             ggCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCa                                                                                                   <  1:943604/149‑1 (MQ=255)
                                                  cGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAac                                                                                              >  1:710713/1‑150 (MQ=255)
                                                          tGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATg                                                                                      <  2:845190/150‑1 (MQ=255)
                                                            gCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGt                                                                                    <  2:745499/150‑1 (MQ=255)
                                                              gTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTc                                                                                   <  1:692461/149‑1 (MQ=255)
                                                              gTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTc                                                                                   >  2:692461/1‑149 (MQ=255)
                                                                    ttCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTg                                                                            <  2:700963/150‑1 (MQ=255)
                                                                        tGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACt                                                                        >  2:745017/1‑150 (MQ=255)
                                                                               aTGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCt                                                                                  >  2:331184/1‑133 (MQ=255)
                                                                               aTGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCt                                                                                  <  1:331184/133‑1 (MQ=255)
                                                                                      tttGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGcc                                                                                                                                              >  2:878623/1‑66 (MQ=255)
                                                                                      tttGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGcc                                                                                                                                              <  1:878623/66‑1 (MQ=255)
                                                                                       ttGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtagt                                                         <  1:1054828/150‑1 (MQ=255)
                                                                                        ggCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCGTCGCGGCTTTAGCCGCGAAGAGGGCGTGACAGGCGCACTCCGCGGGGTGGTCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACGgtagta                                                        <  2:21396/149‑1 (MQ=255)
                                                                                          cAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCAAAAAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtagtagt                                                      >  1:131234/1‑150 (MQ=255)
                                                                                           aGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtagtagta                                                     <  2:1063850/150‑1 (MQ=255)
                                                                                                  ccTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGc                                                                                                                          <  2:1207814/74‑1 (MQ=255)
                                                                                                  ccTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGc                                                                                                                          >  1:1207814/1‑74 (MQ=255)
                                                                                                  ccTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCt                                                                                  >  2:236782/1‑114 (MQ=255)
                                                                                                  ccTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCt                                                                                  <  1:236782/114‑1 (MQ=255)
                                                                                                  ccTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTTGAGTAGTACCCCTGa                                              <  2:710713/150‑1 (MQ=255)
                                                                                                    tGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGc                                            >  2:251333/1‑150 (MQ=255)
                                                                                                                gagGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAac                                                                                              <  2:301387/88‑1 (MQ=255)
                                                                                                                gagGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAac                                                                                              >  1:301387/1‑88 (MQ=255)
                                                                                                                           cggcggCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCg                     >  2:365443/1‑150 (MQ=255)
                                                                                                                                     ccTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGAt           <  1:251333/150‑1 (MQ=255)
                                                                                                                                     ccTCTTCGCGGCTGAAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTgg                                                                           <  2:142458/86‑1 (MQ=255)
                                                                                                                                     ccTCTTCGCGGCTGAAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTgg                                                                           >  1:142458/1‑86 (MQ=255)
                                                                                                                                         ttCGCGGCTGAAGCCGCGAAGAGGTCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCgg                                                                  <  2:740507/91‑1 (MQ=38)
                                                                                                                                         ttCGCGGCTGAAGCCGCGAAGAGGTCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCgg                                                                  >  1:740507/1‑91 (MQ=38)
                                                                                                                                         ttCGCGGCTGAAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATgcgc       >  2:1171240/1‑150 (MQ=255)
                                                                                                                                              ggCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGctgctg  <  1:659692/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AAAAAAGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTG  >  NC_002947/1474856‑1475147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑