Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	181,943	C→T	24.7%	noncoding (52/120 nt)	PP_5SB →	5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	181,943	0	C	T	24.7%	103.0 / 40.7	69	noncoding (52/120 nt)	PP_5SB	5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (26/26);  new base T (11/6);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-01

AAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCT  >  NC_002947/181795‑182090                                                                                                                                                     |                                                                                                                                                     aaaaCCCCTATCTGCACATGCAGGTAGGGGGTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTcagca                                                                                                                                                     >  1:729431/1‑150 (MQ=255)   aaCCCCTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGt                                                                                                                                                   >  2:621889/1‑150 (MQ=37)     ccccTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGa                                                                                                                                                 >  2:600642/1‑150 (MQ=37)      cccTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTAGCACGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGaa                                                                                                                                                >  2:164260/1‑150 (MQ=25)       ccTATCTGCACATGCAGGGAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCACGAAATAAGCAGTGaaa                                                                                                                                               >  2:535405/1‑150 (MQ=255)                      aGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCATGTTAATGCTACGGACACCCCAGCAAACAACACTTGTTCTCGAACTCAGAGCATAGACGCCGCCTGACCGCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGAt                                                                                                                                <  2:48531/150‑1 (MQ=12)                       gatAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATg                                                                                                                               <  1:627646/148‑1 (MQ=255)                            gggTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAgt                                                                                                                          >  1:955443/1‑150 (MQ=255)                               ttttGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGtgtg                                                                                                                       >  2:675767/1‑150 (MQ=255)                               ttttGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGtgtg                                                                                                                       >  2:702623/1‑150 (MQ=255)                                 ttGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTggg                                                                                                                     <  1:600642/150‑1 (MQ=255)                                 ttGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACCACCATAGAGCATTGGAACCCCCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTggg                                                                                                                     <  2:194894/150‑1 (MQ=255)                                       ttAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTcc                                                                                                               <  2:729431/150‑1 (MQ=255)                                               gAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTga                                                                                                       <  1:883325/150‑1 (MQ=255)                                               gAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTga                                                                                                       <  2:189324/150‑1 (MQ=255)                                                    aCAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCGTGTGAGAGTa                                                                                                  >  1:1140750/1‑150 (MQ=255)                                                     cAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTccc                                                                                                              >  1:331204/1‑137 (MQ=255)                                                     cAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTccc                                                                                                              <  2:331204/137‑1 (MQ=255)                                                      agagATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCGTGTGAGAGTAgg                                                                                                <  2:1140750/150‑1 (MQ=255)                                                          aTTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCAt                                                                                            >  2:921469/1‑150 (MQ=255)                                                                      gTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCa                                                                                <  1:355836/150‑1 (MQ=255)                                                                      gTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTAGAACCCCCGGAGCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCa                                                                                >  2:767292/1‑150 (MQ=255)                                                                        ccTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCaaa                                                                              <  1:702623/150‑1 (MQ=255)                                                                              gACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTATCCCCATGAGCGAGACGGTCATCGTGAAGATTCAAACCaaaa                                                                        >  2:1187881/1‑150 (MQ=14)                                                                                       gCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAGGATTCAAACCCAAAGCCCCTGTc                                                               >  2:484378/1‑150 (MQ=255)                                                                                             aGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcat                                                         <  1:268950‑M2/150‑24 (MQ=255)                                                                                              gAATTTCTTGACGACCAGAGAGCATTGGAACCACCTGATCCAATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATGGTCGAGATTCAAACCCCAAGCCCCTGTCTGCGAT‑g                                                       >  2:482961/1‑150 (MQ=17)                                                                                                 tttCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAg                                                    >  2:282742/1‑150 (MQ=255)                                                                                                     ttGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAg                                                <  1:164260/150‑1 (MQ=255)                                                                                                      tGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcgtgcgaagata                                                <  1:36993‑M2/150‑33 (MQ=255)                                                                                                       gacgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcgtgcgcagatag                                               >  2:990081‑M2/1‑117 (MQ=255)                                                                                                       gacgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgtgcagatag                                               >  1:606447‑M2/1‑117 (MQ=255)                                                                                                       gacgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAggg                                              <  1:282742/150‑1 (MQ=255)                                                                                                        acgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATTGTCAAGAGTCAtttcacaaaacccctacctgcgtgcgcagatagg                                              >  1:511708‑M2/1‑116 (MQ=255)                                                                                                         cgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcgtgcgcagataggg                                             >  1:458733‑M2/1‑115 (MQ=255)                                                                                                         cgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcgtgcgaagataggg                                             >  2:36993‑M2/1‑115 (MQ=255)                                                                                                         cgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagataggg                                             >  1:1120041‑M2/1‑115 (MQ=255)                                                                                                         cgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGc                                            <  1:921469/150‑1 (MQ=255)                                                                                                          gacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcgtgcgcagatagggg                                            <  2:4718‑M2/150‑37 (MQ=255)                                                                                                          gacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcacaaaacccctacctgcgtgcgcagatagg                                              >  1:998605‑M2/1‑114 (MQ=255)                                                                                                          gacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcacaaaacccctacctgcgtgcgcagatagg                                              <  2:998605‑M2/148‑35 (MQ=255)                                                                                                          gacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGACGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagatagggg                                            >  1:721055‑M2/1‑114 (MQ=255)                                                                                                           acCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagataggggt                                           >  2:268950‑M2/1‑113 (MQ=255)                                                                                                             cATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgtgcagatagggg                                            <  1:1158245‑M2/147‑37 (MQ=255)                                                                                                             cATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgtgcagatagggg                                            >  2:1158245‑M2/1‑111 (MQ=255)                                                                                                             cATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATC‑GCAGACAgg                                               <  2:995578/143‑1 (MQ=255)                                                                                                             cATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGC‑ATCGCAGACAgg                                               >  1:995578/1‑143 (MQ=255)                                                                                                                 gagCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAggg                                              >  1:838738/1‑140 (MQ=255)                                                                                                                 gagCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAggg                                              <  2:838738/140‑1 (MQ=255)                                                                                                                  agCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTc                                                                                 <  2:309164/105‑1 (MQ=33)                                                                                                                  agCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTc                                                                                 >  1:309164/1‑105 (MQ=33)                                                                                                                    cATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTc                                 <  1:329801/150‑1 (MQ=255)                                                                                                                       tGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCgg                              <  1:675767/150‑1 (MQ=255)                                                                                                                         gtgccacaTGAACGCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGt                            <  1:621889/147‑1 (MQ=255)                                                                                                                         gAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATc                                                                                           >  1:745532/1‑88 (MQ=35)                                                                                                                         gAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATc                                                                                           <  2:745532/88‑1 (MQ=35)                                                                                                                         gAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGt                            >  2:208455/1‑150 (MQ=255)                                                                                                                          aaCCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcgtgcgcagataggg                                             <  2:1072554‑M2/133‑36 (MQ=255)                                                                                                                          aaCCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcgtgcgcagataggg                                             >  1:1072554‑M2/1‑98 (MQ=255)                                                                                                                            ccaccTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTc                         >  1:1008771/1‑150 (MQ=255)                                                                                                                             caccTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAAc                                                                             >  2:1179767/1‑98 (MQ=38)                                                                                                                             caccTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAAc                                                                             <  1:1179767/98‑1 (MQ=38)                                                                                                                                cTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAata                     >  2:1011180/1‑150 (MQ=255)                                                                                                                                  gATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAgtgt                                                                                                                        <  2:953593/50‑1 (MQ=38)                                                                                                                                      ccatcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGtt               >  2:608529/1‑150 (MQ=255)                                                                                                                                      ccatcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACACAAAGCCCCTGTCTGC‑ATCGCAGACAg                                                <  1:982665/117‑1 (MQ=17)                                                                                                                                      ccatcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACACAAAGCCCCTGTCTGC‑ATCGCAGACAg                                                >  2:982665/1‑117 (MQ=17)                                                                                                                                         tcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAC‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGa            >  2:414345/1‑150 (MQ=255)                                                                                                                                                 tCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAg    <  1:767292/150‑1 (MQ=255)                                                                                                                                                 tCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAg    <  1:208455/150‑1 (MQ=255)                                                                                                                                                  cagcagTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGc   >  2:200102/1‑150 (MQ=255)                                                                                                                                                   agcagTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACATGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCt  >  1:1061021/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                     AAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCT  >  NC_002947/181795‑182090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑