Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,489,377 A→G 6.7% intergenic (+169/‑34) ybgI → / → cysD metal‑binding hydrolase/oxidase/sulfate adenylyltransferase subunit 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,489,3770AG 6.7% 174.7 / 2.4 60intergenic (+169/‑34)ybgI/cysDmetal‑binding hydrolase/oxidase/sulfate adenylyltransferase subunit 2
Reads supporting (aligned to +/- strand):  ref base A (24/32);  new base G (2/2);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-01

GCGGGCCTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTACTCGATCGGCAAGGAC  >  NC_002947/1489229‑1489524
                                                                                                                                                    |                                                                                                                                                   
gcgGGCCTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAg                                                                                                                                                    <  2:591488/150‑1 (MQ=255)
   ggCCTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGcc                                                                                                                                                 >  2:761365/1‑150 (MQ=255)
      cTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTc                                                                                                                                              >  1:535975/1‑150 (MQ=255)
         aTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCAt                                                                                                                                           >  1:1000373/1‑150 (MQ=255)
             gAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGa                                                                                                                                       <  1:1009256/150‑1 (MQ=255)
                   gTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAg                                                                                                                                                    <  1:1073211/131‑1 (MQ=255)
                   gTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAg                                                                                                                                                    >  2:1073211/1‑131 (MQ=255)
                      cACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTc                                                                                                                              <  2:535975/150‑1 (MQ=255)
                      aaCCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTc                                                                                                                              <  1:1078988/149‑1 (MQ=255)
                       aCCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATTGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCg                                                                                                                             >  2:245972/1‑150 (MQ=255)
                         ccGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTg                                                                                                                           >  2:639146/1‑150 (MQ=255)
                          cgcgAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGa                                                                                                                          <  2:1146751/150‑1 (MQ=255)
                          cgcgAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGa                                                                                                                          <  2:778808/150‑1 (MQ=255)
                           gcgAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGttt                                                                                                                                 >  2:171511/1‑142 (MQ=255)
                           gcgAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGttt                                                                                                                                 <  1:171511/142‑1 (MQ=255)
                                gagGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATTGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGcc                                                                                                                    <  1:33997/150‑1 (MQ=255)
                                        cccTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCTGCGGGCCGtgttc                                                                                                                                           >  1:227454/1‑115 (MQ=255)
                                        cccTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCTGCGGGCCGtgttc                                                                                                                                           <  2:227454/119‑5 (MQ=255)
                                         ccTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCGATAAGATCGTTTTTTGTGAGGAGCCATGGTCGAc                                                                                                           >  1:1031279/1‑150 (MQ=255)
                                                gCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGACCTCGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGa                                                                                                    <  1:854588/150‑1 (MQ=255)
                                                       cccccAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACtt                                                                                             <  2:1054117/150‑1 (MQ=255)
                                                                       tAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCg                                                                             <  1:959767/150‑1 (MQ=255)
                                                                          aCTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGgag                                                                          >  2:255873/1‑150 (MQ=255)
                                                                                  gATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCca                                                                  <  1:245972/150‑1 (MQ=255)
                                                                                   aTCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCcac                                                                 >  2:784590/1‑150 (MQ=255)
                                                                                       agccagccTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCAcatca                                                             >  2:172692/1‑150 (MQ=255)
                                                                                        gccagccTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCAcatcat                                                            >  2:877774/1‑150 (MQ=255)
                                                                                           agccTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGgaga                                                                         <  1:187501/134‑1 (MQ=255)
                                                                                           agccTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGgaga                                                                         >  2:187501/1‑134 (MQ=255)
                                                                                             cctcctAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGc                                                                                     <  1:87732/120‑1 (MQ=255)
                                                                                             cctcctAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGc                                                                                     >  2:87732/1‑120 (MQ=255)
                                                                                             cctcctAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGc                                                                       >  2:214166/1‑134 (MQ=255)
                                                                                             cctcctAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGc                                                                       <  1:214166/134‑1 (MQ=255)
                                                                                              ctcctAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGa                                                      <  2:191325/150‑1 (MQ=255)
                                                                                                 ctAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTCGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGt                                                   <  2:850064/150‑1 (MQ=255)
                                                                                                 ctAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGGCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTTACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGt                                                   <  1:864437/150‑1 (MQ=255)
                                                                                                 ctAAATAGAAGCAGCTACTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGt                                                   >  2:748953/1‑150 (MQ=255)
                                                                                                  tAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTg                                                  <  1:784590/150‑1 (MQ=255)
                                                                                                   aaaTAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTgg                                                 >  2:571851/1‑150 (MQ=255)
                                                                                                   aaaTAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTgg                                                 >  2:572346/1‑150 (MQ=255)
                                                                                                          agcagcTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGa                                          <  2:1000373/150‑1 (MQ=255)
                                                                                                              gctgctGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTc                                      >  2:1149904/1‑150 (MQ=255)
                                                                                                                  ctgTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCt                                                                                    <  2:667576/100‑1 (MQ=255)
                                                                                                                  ctgTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCt                                                                                    >  1:667576/1‑100 (MQ=255)
                                                                                                                    gtgATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAAc                                >  2:36287/1‑150 (MQ=255)
                                                                                                                           aGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGa                         <  1:255873/150‑1 (MQ=255)
                                                                                                                            gTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGAt                        <  1:572346/150‑1 (MQ=255)
                                                                                                                            gTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGAt                        >  1:1171671/1‑150 (MQ=255)
                                                                                                                            gTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGAt                        <  1:571851/150‑1 (MQ=255)
                                                                                                                             tGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGATCGCCGCCGAGTTCGACAACCCGGTGATg                       <  1:521829/150‑1 (MQ=255)
                                                                                                                              ggCTCGGTCGAGCACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGc                      <  2:1031279/150‑1 (MQ=255)
                                                                                                                               gctcgctcGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGAc                                   >  2:1034679/1‑136 (MQ=255)
                                                                                                                               gctcgctcGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGAc                                   <  1:1034679/136‑1 (MQ=255)
                                                                                                                                   gctcgAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTAc                 <  1:639146/150‑1 (MQ=255)
                                                                                                                                     tcgAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACaaa                                                                                                        >  1:879303/1‑61 (MQ=255)
                                                                                                                                     tcgAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACaaa                                                                                                        <  2:879303/61‑1 (MQ=255)
                                                                                                                                        aaCACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTACTCGAt            <  2:718678/150‑1 (MQ=255)
                                                                                                                                         ggacGGCCTGCGGGCCGTCCATAAGACCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTACTCGATc           >  1:175474/3‑150 (MQ=255)
                                                                                                                                          gacGGCCTGCGGGCCGTCCATAAGACCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTACTCGATCg          <  2:175474/149‑1 (MQ=255)
                                                                                                                                          cacGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTACTCGATCg          <  1:172692/150‑1 (MQ=255)
                                                                                                                                                  ggaGGCCGTCTATAGGGTCGTGTTTTGTGGGTAGCCGTGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGGGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTACTCGATCGGCAAGGAc  <  1:508273/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCGGGCCTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTACTCGATCGGCAAGGAC  >  NC_002947/1489229‑1489524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑