Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,497,846 C→A 18.0% L143M (CTG→ATG)  PP_1310 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,497,8460CA18.0% 87.9 / 10.1 38L143M (CTG→ATG) PP_1310hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (16/15);  new base A (3/4);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

CTGCGTGCCAGGCACCCCGGGCTACCGCTGGTGCTGATGGGCTTTTCGTTCGGTGGTTTCGTTGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTCCCCATGAGCTGCTGAAAGTGGCAGAATGCGGACAC  >  NC_002947/1497702‑1497995
                                                                                                                                                |                                                                                                                                                     
cTGCGTGCCAGGCACCCCGGGCTACCGCTGGTGCTGATGGGCGTTTCGTTCGGTGGTTTCGTTGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCAGCTGTTCATGATCGCCCCGGCGGTCAGGCGCATGAcc                                                                                                                                                  <  2:950038/150‑1 (MQ=255)
 tGCGTGCCAGGCACCCCGGGCTACCGCTGGTGCTGATGGGCTTTTCGTTCGGTGGTTTCGTTGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGAccg                                                                                                                                                 >  2:610033/1‑150 (MQ=255)
     tGCCAGGCACCCCGGGCTACCGCTGGTGCTGATGGGCTTTTCGTTCGGTGGTTTCGTTGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCAGGCGCATGAccgccgg                                                                                                                                             <  2:964818/150‑2 (MQ=255)
             aCCCCGGGCTACCGCTGGTGCTGATGGGCTTTTCGTTCGGTGGTTTCGTTGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGa                                                                                                                                     >  2:1014083/1‑150 (MQ=255)
             aCCCCGGGCTACCGCTGGTGCTGATGGGCTTTTCGTTCGGTGGTTTCGTTGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGa                                                                                                                                     <  1:415840/150‑1 (MQ=255)
                                  tGATGGGCTTTTCGTTCGGTGGTTTCGTTGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCa                                                                                                                >  2:1156381/1‑150 (MQ=255)
                                    aTGGGCTTTTCGTTCGGTGGTTTCGTTGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCTATGCCGCAGCGCTGCCCGCTCAcc                                                                                                              >  1:284713/1‑150 (MQ=255)
                                       ggCTTTTCGTTCGGTGGTTTCGTTGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCgtg                                                                                                           <  1:325401/150‑1 (MQ=255)
                                                         ttCGTTGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCTATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGAt                                                                                         <  2:284713/150‑1 (MQ=255)
                                                            gTTGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCAGGCGCATGAccgccgg                                                                                                                                             >  1:488851/1‑94 (MQ=255)
                                                            gTTGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCAGGCGCATGAccgccgg                                                                                                                                             <  2:488851/95‑2 (MQ=255)
                                                             ttGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAg                                                                                     >  1:437431/1‑150 (MQ=255)
                                                               gCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGCCCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCGCACCGTGGTGCAGCCGGACGCCGATGAAgtc                                                                                   <  2:740298/150‑1 (MQ=255)
                                                                    caGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTcgc                                                                              >  1:51196/1‑150 (MQ=255)
                                                                      gCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCgcgc                                                                            <  1:1148935/150‑1 (MQ=255)
                                                                                      tGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGa                                                            >  1:415180/1‑150 (MQ=255)
                                                                                        aaaCTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAAt                                                          <  1:1040136/150‑1 (MQ=255)
                                                                                           cTGCAGGCGTCGAGTTGCAGCACCTGTTCACGCTTCCGATCTCTGCGACTCGCCGGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGt                                                       <  1:870882/150‑1 (MQ=255)
                                                                                                   gTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGc                                               >  2:405411/1‑150 (MQ=255)
                                                                                                    tCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGcc                                              <  2:415180/150‑1 (MQ=255)
                                                                                                         ttGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTcg                                         <  2:51196/150‑1 (MQ=255)
                                                                                                             agcaCCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTc                                     <  1:610033/150‑1 (MQ=255)
                                                                                                              gcaCCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTcc                                    >  2:242093/1‑150 (MQ=255)
                                                                                                               caCCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGAt                                                                                         <  2:552636/96‑1 (MQ=255)
                                                                                                               caCCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGAt                                                                                         >  1:552636/1‑96 (MQ=255)
                                                                                                                    gTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTCCCCATGa                              <  1:1014083/150‑1 (MQ=255)
                                                                                                                     ttCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTCCCCATGAg                             >  1:490167/1‑150 (MQ=255)
                                                                                                                        aTGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTCCCCATGAgctg                          >  2:840508/1‑150 (MQ=255)
                                                                                                                             cGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTCCCCATGAGCTGCTGaa                     >  1:6386/1‑150 (MQ=255)
                                                                                                                                  cggcggTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTCCCCATGAGCTGCTGAAAGTgg                <  1:1156381/150‑1 (MQ=255)
                                                                                                                                   ggcggTCAGGCGCATGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTcgc                                        >  2:603397/1‑125 (MQ=255)
                                                                                                                                   ggcggTCAGGCGCATGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTcgc                                        <  1:603397/125‑1 (MQ=255)
                                                                                                                                   ggcggTCAGGCGCATGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTCCCCATGAGCTGCTGAAAGTGGc               >  1:614892/1‑150 (MQ=255)
                                                                                                                                    gcggTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTCCCCATGa                              <  1:582033/134‑1 (MQ=255)
                                                                                                                                    gcggTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTCCCCATGa                              >  2:582033/1‑134 (MQ=255)
                                                                                                                                        tCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAgtcgtc                                                                                >  2:270894/1‑80 (MQ=255)
                                                                                                                                        tCATGCCCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAgtcgtc                                                                                <  1:270894/80‑1 (MQ=255)
                                                                                                                                          aTGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTCCCCATGAGCTGCTGAAAGTGGCAGAATGc        >  1:735103/1‑150 (MQ=255)
                                                                                                                                             ccccTGACCTCCGAATCCCCGACGACGCAGCGGTGCCCGCTCACCGTCGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACTAATGGTCCGACAGCCTGTCGCGTCCCCATGAGCTGCTGAAAGTGGCAGAATGCGGa     <  2:423468/148‑1 (MQ=255)
                                                                                                                                                cTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTCCCCATGAGCTGCTGAAAGTGGCAGAATGCGGacac  <  2:437431/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
CTGCGTGCCAGGCACCCCGGGCTACCGCTGGTGCTGATGGGCTTTTCGTTCGGTGGTTTCGTTGCCACCAGCCTGGCCGGGCGCCTTGAAACTGCAGGCGTCGAGTTGCAGCACCTGTTCATGATCGCCCCGGCGGTCATGCGCCTGACCGCCGAATTCCCGATGCCGCAGCGCTGCCCGCTCACCGTGGTGCAGCCGGACGCCGATGAAGTCGTCGCGCCGCAGCTGGTTTACGAATGGTCCGACAGCCTGTCGCGTCCCCATGAGCTGCTGAAAGTGGCAGAATGCGGACAC  >  NC_002947/1497702‑1497995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑