| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,503,475 | A→C | 13.5% | E90A (GAG→GCG) ‡ | rplM → | 50S ribosomal protein L13 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,503,475 | 0 | A | C | 13.5% | 119.6 / 14.3 | 52 | E90A (GAG→GCG) ‡ | rplM | 50S ribosomal protein L13 |
| Reads supporting (aligned to +/- strand): ref base A (24/21); new base C (3/4); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01 | |||||||||||
AACCAGAATACACCCCTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTCGCGACATGTACCGCAAGCTGAAAGTGTACGCGGGTGCTGCTCACCCACACACTGCTCAGCAGC > NC_002947/1503328‑1503615 | aaCCAGAATACACCCCTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCgaga > 2:334323/1‑150 (MQ=255) aCCAGAATACACCCCTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGaa < 2:1095345/150‑1 (MQ=255) aGAATACACCCCTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGtt > 1:129196/1‑150 (MQ=255) aaTACACCCCTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCTCGAAGTTGa < 1:245987/150‑1 (MQ=255) tACACCCCTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATc < 2:129196/150‑1 (MQ=255) acCCCTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGa > 1:382505/1‑145 (MQ=255) acCCCTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGa < 2:382505/145‑1 (MQ=255) acCCCTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGcc > 2:946180/1‑150 (MQ=255) cccTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCCCTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCaa < 2:1175786/150‑1 (MQ=255) cccTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCTCGAAGTTGAtttcctt < 2:509143/150‑7 (MQ=255) cccTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCTCGAAGTTGAtttcctt < 2:1112593/150‑7 (MQ=255) cccTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCaa > 2:202210/1‑150 (MQ=255) ccTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAg > 2:276595/1‑150 (MQ=255) cTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGa > 1:582171/1‑150 (MQ=255) gtcgtTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCga > 2:468669/1‑107 (MQ=255) gtcgtTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCga < 1:468669/107‑1 (MQ=255) tcgtTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCgg > 2:972857/1‑150 (MQ=255) ttATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCa > 1:436572/1‑150 (MQ=255) aTCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAgg > 1:672275/1‑118 (MQ=255) aTCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAgg < 2:672275/118‑1 (MQ=255) aaCGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGc < 1:939926/150‑1 (MQ=255) gCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCAtg > 2:44545/1‑150 (MQ=255) atgTTCGTGTGACTGGTGACAAGTCTTCCGACAAAATGTACTACTACCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTGCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGACTa < 2:149603/148‑1 (MQ=255) gTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCTCGAAGTTGAtttcc > 1:1150521/1‑95 (MQ=255) gTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCTCGAAGTTGAtttcc < 2:1150521/99‑5 (MQ=255) gTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTAATACCAAACAGGTTTCCCAGGCGGTATCAAGTAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAg > 1:328817/1‑150 (MQ=255) cGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAAc < 1:276595/150‑1 (MQ=255) aCTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTg < 1:334323/150‑1 (MQ=255) cTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTgg > 2:667356/1‑150 (MQ=255) tGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTggg < 1:44545/150‑1 (MQ=255) gCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTcgc < 1:946180/150‑1 (MQ=255) ccAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAAccc > 1:678373/1‑139 (MQ=255) ccAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAAccc < 2:678373/139‑1 (MQ=255) ccAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTggg > 2:1158799/1‑145 (MQ=255) ccAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTggg < 1:1158799/145‑1 (MQ=255) gACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTCGCGACATGTACCGc > 1:397187/1‑150 (MQ=255) gACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTCGCGACATGTACCGc > 1:182082/1‑150 (MQ=255) aCAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGc > 2:966698/1‑110 (MQ=255) aCAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGc < 1:966698/110‑1 (MQ=255) gTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTAt < 1:71097/84‑1 (MQ=255) gTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTAt > 2:71097/1‑84 (MQ=255) ctactCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTCGCGACATGTACCGCAAGCTGAAAgt > 2:1181451/1‑150 (MQ=255) ctCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCg < 1:939874/115‑1 (MQ=255) ctCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCg > 2:939874/1‑115 (MQ=255) tCCCACTCCGGTTTCCCAGGCGGTATCAAGGTAATAGATTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTCGCGACATGTACCGCAAGCTGAAAGTGTAc < 2:400888/150‑1 (MQ=255) tCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTCGCGACATGTACCGCAAGCTGAAAGTGTAc < 1:939434/150‑1 (MQ=255) ccACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTCGCGACATGTACCGCAAGCTGAAAGTGTAcgc > 2:291134/1‑150 (MQ=255) ccACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTGCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTCGCGACATGTACCGCAAGCTGAAAGTGTAcga > 1:400888/1‑149 (MQ=255) cACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGCTCGCGACATGTACCGCAAGCTGAAAGTGTAcgcg > 1:503957/1‑150 (MQ=255) cAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTCGCGACATGTACCGCAAGCTGAAAGTGTACGCGGGTGCTGCTCAcc < 1:972857/150‑1 (MQ=255) aaGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCt < 1:843949/89‑1 (MQ=255) aaGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCt > 2:843949/1‑89 (MQ=255) aGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTCGCGACATGTACCGCAAGCTGAAAGTGTACGCGGGTGCTGCTCACCCACACACTGCTc < 1:668574/150‑1 (MQ=255) gaTCAACTTCTCGAAGTTGATCGCCAAGGCCCCTGAGCGTGGGGGCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTCGCGACATGTACCGCAAGCTGAAAGTGTACGCGGGTGCTGCTCACCCACACACTGCTcagca > 2:834790/2‑150 (MQ=255) tCAACTTCTCGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTCGCGACATGTACCGCAAGCTGAAAGTGTACGCGGGTGCTGCTCACCCACACACTGCTCagcagc > 2:821650/1‑150 (MQ=255) | AACCAGAATACACCCCTCACGTTGACACCGGCGACTACATCGTCGTTATCAACGCCGAGCAGGTTCGTGTGACTGGTGCCAAGTCTTCCGACAAAATGTACTACTCCCACTCCGGTTTCCCAGGCGGTATCAAGGAAATCAACTTCGAGAAGTTGATCGCCAAGGCCCCTGAGCGTGTTATCGAAACTGCGGTCAAAGGCATGCTGCCTAAGAACCCGCTGGGTCGCGACATGTACCGCAAGCTGAAAGTGTACGCGGGTGCTGCTCACCCACACACTGCTCAGCAGC > NC_002947/1503328‑1503615 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |