| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,505,553 | A→C | 12.3% | D219A (GAC→GCC) | petB → | cytochrome b |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,505,553 | 0 | A | C | 12.3% | 116.0 / 11.5 | 49 | D219A (GAC→GCC) | petB | cytochrome b |
| Reads supporting (aligned to +/- strand): ref base A (23/20); new base C (2/4); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.40e-01 | |||||||||||
TTCGTGGTGACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATGG > NC_002947/1505406‑1505693 | ttCGTGGTGACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACa > 2:727309/1‑150 (MQ=255) cGTGGTGACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATc < 1:727309/150‑1 (MQ=255) tggtgACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCag < 2:800028/150‑2 (MQ=255) ggtgACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCagg < 1:365259/150‑3 (MQ=255) tACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGaaa > 2:500212/1‑150 (MQ=255) tACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGaaa > 1:77014/1‑150 (MQ=255) tACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTATCGGTCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGaaa > 1:572050/1‑150 (MQ=255) tGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAgg > 1:676224/1‑150 (MQ=255) gATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGa < 2:207866/150‑1 (MQ=255) cATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGCACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACgg < 1:280466/150‑1 (MQ=255) ccTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGA‑AGCACACGTGAGAAAGGACGAAAACGGTATTcc > 1:604919/1‑150 (MQ=255) ccGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACAAAAACGGTATTCCGCTGGa < 2:676224/150‑1 (MQ=255) gCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATg > 1:347067/1‑150 (MQ=255) cttcttCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCag > 1:972122/1‑113 (MQ=255) cttcttCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCag < 2:972122/114‑2 (MQ=255) gCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCt > 1:9778/1‑137 (MQ=255) gCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCt < 2:9778/137‑1 (MQ=255) ccTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGtt < 2:347067/150‑1 (MQ=255) ccTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGAATGGTATTCCGtt > 2:510571/1‑150 (MQ=255) ccTCCATGTGGTTGCCCTGCCAATCGTCATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTTGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGtt < 1:436842/150‑1 (MQ=255) cTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGCTGGAGTCGACATCA‑AGAAGAGAGGTGCGGAGGTCATGAGTCCGCTGGATGGTATTCCGTTCCACCCGTACTACAcc < 1:510571/150‑1 (MQ=255) cTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACAcc > 2:857837/1‑150 (MQ=255) gCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGt > 1:1104423/1‑150 (MQ=255) aTCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCCTACTACACCGTTaaa < 1:339188/150‑1 (MQ=255) tGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCagg > 2:605138/1‑78 (MQ=255) tGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCagg < 1:605138/80‑3 (MQ=255) gtggtATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCgtggtg > 1:509573/1‑150 (MQ=255) tATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGgtgt > 1:40054/1‑150 (MQ=255) aTTGCACATCCTCGCCGTGCACGAAATGGGTTCGAACAACGCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCACTGAATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGCCATTGTCGGCGTGGTGGTGtt < 2:77014/150‑1 (MQ=255) cTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTg > 1:1186590/1‑150 (MQ=255) cACGAAGTGTGTTCGAACAACCCGGATGGCGTCGACATAA‑AGAATAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGcc < 2:509573/150‑1 (MQ=255) cACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTa < 1:511713/64‑1 (MQ=255) cACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTa > 2:511713/1‑64 (MQ=255) aCGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCTGCGGGGGGGTGTGCCTGTTCGTGTTCTGCGACg > 2:197592/1‑150 (MQ=255) gAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCgtg > 2:197689/1‑120 (MQ=255) gAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCgtg < 1:197689/120‑1 (MQ=255) aGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCgtggt < 1:857837/150‑1 (MQ=255) ggTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGttc < 2:40054/150‑1 (MQ=255) ttCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAATAAAGTTATCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGttctt > 1:676895/1‑150 (MQ=255) ttCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGttctt > 2:1029416/1‑150 (MQ=255) aacaacCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCgg > 2:178650/1‑104 (MQ=255) aacaacCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCgg < 1:178650/104‑1 (MQ=255) aacCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGAt < 2:236694/57‑1 (MQ=255) aacCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGAt > 1:236694/1‑57 (MQ=255) cccTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGa > 1:73053/1‑150 (MQ=255) ccTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGaa > 2:655440/1‑150 (MQ=255) gATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATg < 1:197592/150‑1 (MQ=255) aTGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATgg < 1:500212/150‑1 (MQ=255) aTGGCGGCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATgg < 2:1104423/150‑1 (MQ=255) | TTCGTGGTGACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCA‑AGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATGG > NC_002947/1505406‑1505693 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |