Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,570,373 G→C 15.4% G381A (GGC→GCC)  pcaF‑I → beta‑ketoadipyl‑CoA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,570,3730GC15.4% 117.4 / 7.3 43G381A (GGC→GCC) pcaF‑Ibeta‑ketoadipyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base G (14/22);  new base C (3/4);  total (17/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGA  >  NC_002947/1570228‑1570516
                                                                                                                                                 |                                                                                                                                               
ctgcGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAATCCCTAATGGCGGTGCCATTCCCCTGTGCCACCTCCTGGGCATGAGCGGTGCACGCCTGGTACGGACTGCGTTGCACCACCTCGAGAAGAGTGGCGGTCGCAAGGCCCTg                                                                                                                                             <  2:846/150‑1 (MQ=255)
        gCTGGGTGTGGCTGACGATGCGCCGCAGGTAAACACTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTAACTGCGTTGAACCAGCTGGAGAAGGGTGGCGTGCGCAAGGGCCTGGTGACCAt                                                                                                                                     <  2:18511/150‑1 (MQ=255)
         cTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCCAGGCCCtt                                                                                                                                             <  1:1069057/141‑2 (MQ=255)
         cTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCCAGGCCCtt                                                                                                                                             >  2:1069057/1‑140 (MQ=255)
              tgtgGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCAtgtgtgt                                                                                                                               >  2:908405/1‑150 (MQ=255)
                tgGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTgg                                                                                                                             <  2:1093470/150‑1 (MQ=255)
                    tGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCAt                                                                                                                                     >  2:600212/1‑138 (MQ=255)
                    tGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCAt                                                                                                                                     <  1:600212/138‑1 (MQ=255)
                        gATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGc                                                                                                                     <  2:1195992/150‑1 (MQ=255)
                                       aaCCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTg                                                                                                      <  2:1009628/150‑1 (MQ=255)
                                            tAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCAt                                                                                                 >  1:1162379/1‑150 (MQ=255)
                                                 gcaccgCCATTGCCCTGGGCCGCCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGc                                                                                            <  2:330285/145‑1 (MQ=255)
                                                 gCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGc                                                                                            >  1:698301/1‑150 (MQ=255)
                                                       ccATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGttt                                                                                      <  1:177669/150‑1 (MQ=255)
                                                       ccATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGttt                                                                                      <  1:908405/150‑1 (MQ=255)
                                                        cATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTg                                                                                     >  1:674152/1‑150 (MQ=255)
                                                         aTTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGTGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGa                                                                                    <  2:376891/150‑1 (MQ=255)
                                                         aTTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCgg                                                                                                                      <  1:886890/116‑1 (MQ=255)
                                                         aTTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCgg                                                                                                                      >  2:886890/1‑116 (MQ=255)
                                                          ttGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGAc                                                                                   <  2:90119/150‑1 (MQ=255)
                                                          ttGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGAc                                                                                   >  2:150768/1‑150 (MQ=255)
                                                          ttGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGAc                                                                                   <  1:150768/150‑1 (MQ=255)
                                                              ccTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCCAGGCCCTTGCGAcc                                                                                                                                       >  1:515387/1‑94 (MQ=38)
                                                              ccTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCCAGGCCCTTGCGAcc                                                                                                                                       <  2:515387/94‑1 (MQ=38)
                                                              ccTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCCAGGCCCTTGCGAcc                                                                                                                                       <  2:515452/94‑1 (MQ=39)
                                                              ccTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCCAGGCCCTTGCGAcc                                                                                                                                       >  1:515452/1‑94 (MQ=39)
                                                                        ccccTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCctct                                                                     <  2:674152/150‑1 (MQ=255)
                                                                          ccTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTc                                                                   >  1:456505/1‑150 (MQ=255)
                                                                            tGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTCGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTcgc                                                                 <  2:698301/150‑1 (MQ=255)
                                                                                                cTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAg                                             <  2:320915/150‑1 (MQ=255)
                                                                                                 tGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAgg                                            <  1:89310/150‑1 (MQ=255)
                                                                                                    tACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGAt                                         >  2:253273/1‑150 (MQ=255)
                                                                                                                  cACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGgt                                                                                                                           >  2:60719/1‑54 (MQ=255)
                                                                                                                  cACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGgt                                                                                                                           <  1:60719/54‑1 (MQ=255)
                                                                                                                   aCCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCtg                          >  1:825104/1‑150 (MQ=255)
                                                                                                                        cTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCaa                                                <  1:1116903/123‑1 (MQ=255)
                                                                                                                        cTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCaa                                                >  2:1116903/1‑123 (MQ=255)
                                                                                                                           gagaAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCg                  <  1:978690/150‑1 (MQ=255)
                                                                                                                           gagaAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCg                  <  1:1128320/150‑1 (MQ=255)
                                                                                                                           gagaAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCg                  >  2:978690/1‑150 (MQ=255)
                                                                                                                                gagTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCt                                                                                 >  2:824610/1‑82 (MQ=255)
                                                                                                                                gagTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCt                                                                                 <  1:824610/82‑1 (MQ=255)
                                                                                                                                           gCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGTCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAaga  <  1:253273/150‑1 (MQ=255)
                                                                                                                                                 gCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCgg                 >  2:1126789/1‑129 (MQ=255)
                                                                                                                                                 gCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCgg                 <  1:1126789/129‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGA  >  NC_002947/1570228‑1570516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑