| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 192,705 | C→A | 8.4% | E271D (GAG→GAT) | paxB ← | toxin secretion ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 192,705 | 0 | C | A | 8.4% | 137.1 / 2.2 | 47 | E271D (GAG→GAT) | paxB | toxin secretion ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (20/23); new base A (2/2); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
GCCCAGAGCCAGCGGGAAGGCGATGATCGGTATCCACACCAGGTGCCCGCCAATGATGGCGATGACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAGGTCGGTCTTCTTGCCGGCCAGGTCCAGGCACAGGCCGCGAAGGCCTTT > NC_002947/192558‑192848 | gCCCAGAGCCAGCGGGAAGGCGATGATCGGTATCCACACCAGGTGCCCGCCAATGATGGCGATGACGATCAAAATCGGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTc > 2:1182175/1‑150 (MQ=255) ccAGAGCCAGCGGGAAGGCGATGATCGGTATCCACACCAGGTGCCCGCCAATGATGGCGATGACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGt > 1:680500/1‑150 (MQ=255) gggAAGGCGATGATCGGTATCCACAACAGGTGCCCGCCAATGATGGCGATGACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTgg < 2:162633/150‑1 (MQ=255) ggAAGGCGATGATCGGTATCCACACCAGGGGCCCGCCAATGATGGCGATGACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTTAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTggg < 1:488598/150‑1 (MQ=255) ggCGATGATCGGTATCCACACCAGGTGCCCGCCAATGATGGCGATGACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCaaa < 2:627876/150‑1 (MQ=255) gCGATGATCGGTATCCACACCAGGTGCCCGCCAATGATGGCGATGACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAg < 2:277245/150‑1 (MQ=255) aGGTGCCCGCCAATGATGGCGATGACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGccggccgg < 2:760341/150‑1 (MQ=255) gCCAATGATGGCGATGACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCAt < 1:410836/150‑1 (MQ=255) gatgGCGATGACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACAt < 2:386555/150‑1 (MQ=255) cGATGACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCcga < 1:921887/150‑1 (MQ=255) gATGACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCGGGCCGGTACTTCATCGACATGCcgac > 1:157134/1‑150 (MQ=255) tGACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCcgacga < 2:798093/150‑1 (MQ=255) gACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGAt > 2:608307/1‑150 (MQ=255) aCGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAAATCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATa > 2:749868/1‑150 (MQ=255) aCGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAAATCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATa > 2:750127/1‑150 (MQ=255) tCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATc > 2:377576/1‑131 (MQ=255) tCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATc < 1:377576/131‑1 (MQ=255) aaaTCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCAGGCGATACGTTCaa > 1:258309/1‑150 (MQ=255) aGGATGGGAAAGTGCAGGTCTATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGGTCTGGGCAAAGCTGCTTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGc < 2:258309/150‑1 (MQ=255) gATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTa < 1:864973/98‑1 (MQ=255) gATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGACTa > 2:864973/1‑98 (MQ=255) tAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCg > 1:1140963/1‑150 (MQ=255) aaaGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCga < 1:1182175/150‑1 (MQ=255) aaaGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCga < 2:680500/150‑1 (MQ=255) aaaGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCga < 2:1140963/150‑1 (MQ=255) aaGGGCAGTTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCgag > 2:805936/1‑150 (MQ=255) aGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAAATCGTGAATGTTCTGGGAACCGCTGCCTACCCGCGCCTGCCTGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATc < 1:750127/150‑1 (MQ=255) aGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAAATCGTGAATGTTCTGGACTGAAGTTCCTACCCGTGCTCTTCCGTTCTTCATCTACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATc < 1:749868/150‑1 (MQ=255) gTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAg > 2:119578/1‑150 (MQ=255) gTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAg > 1:748908/1‑150 (MQ=255) tCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAgg < 2:748908/150‑1 (MQ=255) gATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTgg < 2:887229/67‑1 (MQ=255) gATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTgg > 1:887229/1‑67 (MQ=255) aTCAAGCTGGTCAGGGTCAGCGATGCGAGGAAGTCGAGCAGGCCGTGGAACTCGTGAATGTTCTGGTCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCCACATGACGACGATACGTTCAAACAGCGTTGCCGAGATGATCAggtcg < 2:157134/150‑1 (MQ=255) tCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCACACAGCGTTGCCGAGATGATCAggtcgg > 2:622975/1‑150 (MQ=255) ggCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAGGTCGGtct > 2:917081/1‑150 (MQ=255) gtcagggtcagCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAGGTCGGtctt < 1:9979/146‑1 (MQ=255) gtcagggtcagCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAGGTCGGtctt > 2:9979/1‑146 (MQ=255) tcagCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAGGTCGGTCTTCTTGCCGGCCa < 2:456966/150‑1 (MQ=255) tcagCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAGGTCGGTCTTCTTGCCGGCCa < 1:917081/150‑1 (MQ=255) gcgaggcgagGAAGACGCGCAGGCCCTGGAACTAGTGAACGTTCTGGGCCAAGCGGCCTACGCGCGCCGGCCGGCACTTCATCGAGATGACGACGAGACGTTCAAAAAACGTTGCCGAGATGATCAGGTCGGTCTTCTTGCCGGcatggc > 2:593994/1‑145 (MQ=255) ggcgaggAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAGGTCGGTCTTCTTGCCGGCCAGGTCCAg < 1:610461/150‑1 (MQ=255) tCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAGGTCGGtcttc < 2:732468/123‑1 (MQ=255) tCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAGGTCGGtcttc > 1:732468/1‑123 (MQ=255) gcAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAGGTCGGTCTTCTTGCCGGCCAGGTCCAGGCACAGGCCGCGaa > 1:424795/1‑150 (MQ=255) ccTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAGGTCGGTCTTCTTGCCGGCCAGGTCCAGGCACAGGCCGCGAAGGCCtt > 2:463423/1‑150 (MQ=255) cTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCGTCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAGGTCGGTCTTCTTGCCGGCCAGGTCCAGGCACAGGCCGCGAAGGCCttt > 2:1199252/1‑150 (MQ=255) | GCCCAGAGCCAGCGGGAAGGCGATGATCGGTATCCACACCAGGTGCCCGCCAATGATGGCGATGACGATCAAAATCAGGATGGTAAAGGGCAGGTCGATCAGGCTGGTCAGGGTCAGCGAGGCGAGGAAGTCGCGCAGGCCCTGGAACTCGTGAATGTTCTGGGCAAAGCTGCCTACCCGCGCCGGCCGGTACTTCATCGACATGCCGACGATACGTTCAAACAGCGTTGCCGAGATGATCAGGTCGGTCTTCTTGCCGGCCAGGTCCAGGCACAGGCCGCGAAGGCCTTT > NC_002947/192558‑192848 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |