| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,582,822 | T→A | 17.1% | intergenic (‑226/‑32) | ttgA ← / → ttgR | efflux pump periplasmic linker TtgA/HTH‑type transcriptional regulator TtgR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,582,822 | 0 | T | A | 17.1% | 94.6 / 15.7 | 41 | intergenic (‑226/‑32) | ttgA/ttgR | efflux pump periplasmic linker TtgA/HTH‑type transcriptional regulator TtgR |
| Reads supporting (aligned to +/- strand): ref base T (16/18); new base A (3/4); total (19/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01 | |||||||||||
AATAGCTTGCTAAGGAATATACTTACATTCATGGTTGTTTGTAAATACTGCTGGGTGGTACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCAGATCATCGAGGCGGCGGAAAGGGCCTTCTACAAGCGCGGGGTTGCGCGAACCACCCTGGCCGACATCGCCGAAC > NC_002947/1582682‑1582971 | aaTAGCTTGCTAAGGAATATACTTACATTCATGGTTGTTTGTAAATACTGCTGGGTGGTACTCGGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGAACGCGCAGCGCGTGCg < 1:43380/150‑1 (MQ=255) gCTAAGGAATATACTTACATTCATGGTTGTTTGTAAATACTGCTGGGTGGTACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGAt > 2:1039955/1‑150 (MQ=255) aGGAATATACTTACATTCATGGTTGTTTGTAAATACTGCTGGGTGGTACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTc > 1:1025789/1‑137 (MQ=255) aGGAATATACTTACATTCATGGTTGTTTGTAAATACTGCTGGGTGGTACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTc < 2:1025789/137‑1 (MQ=255) aaTATACTTACATTCATGGTTGTTTGTAAATACTGCTGGGTGGTACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTg > 2:933514/1‑150 (MQ=255) taCTTACATTCATGGTTGTTTGTAAATACTGCTGGGTGGTACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACt < 1:1039955/150‑1 (MQ=255) ttACATTCATGGTTGTTTGTAAATACTGCTGGGTGGTACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGcc < 2:957701/150‑1 (MQ=255) ttgtAAATACTGCTGGGTGGTACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCa > 1:631097/1‑150 (MQ=255) tgtAAATACTGCTGGGTGGTACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCaa < 1:648606/150‑1 (MQ=255) tgctgGGTGGTACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGc > 2:161998/1‑150 (MQ=255) gtggtACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAAc > 2:456644/1‑132 (MQ=255) gtggtACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAAc < 1:456644/132‑1 (MQ=255) gtggtACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGa > 1:953601/1‑150 (MQ=255) aGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGc < 1:490055/150‑1 (MQ=255) aGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCACat < 2:953601/150‑1 (MQ=255) tCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTc > 2:1098244/1‑109 (MQ=255) tCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTc < 1:1098244/109‑1 (MQ=255) tGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCAGATCATCGAggcggc < 2:480939/150‑1 (MQ=255) tGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGACGAACCAAAGAAAAAGCCCCGGCAATCCGTGCCCAGATCATCGAggcggc < 1:933514/150‑1 (MQ=255) aTTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGt > 1:350373/1‑128 (MQ=255) aTTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGt < 2:350373/128‑1 (MQ=255) aGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCAGATCATCGAGGCGGCGGaaa < 1:889689/150‑1 (MQ=255) gCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGccc > 1:58041/1‑113 (MQ=255) gCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGccc < 2:58041/113‑1 (MQ=255) gagaGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGt < 1:973335/116‑1 (MQ=255) gagaGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGt > 2:973335/1‑116 (MQ=255) tcgCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCAGATCATCGAGGCGGCGGAAAGGGCCTTCTACAAGCGCGGGGTTgcg > 2:365421/3‑150 (MQ=255) gTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCAGATCATCGAGGCGGCGGAAAGGGCCTTCTACAAGCGCGGGGTTgcg > 1:243225/1‑150 (MQ=255) gCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCAGATCATCGAGGCGGCGGAAAGGGCCTTCTACAAGCGCGGGGTTGcgcg > 1:137210/1‑150 (MQ=255) ccccGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAAc > 2:973608/1‑91 (MQ=255) ccccGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAAc < 1:973608/91‑1 (MQ=255) ttCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCAGATCATCGAGGCGGCGGAAAGGGCCTTCTACAAGCGCGGGGTTGCGCGAACCACCCTGGc < 1:161998/150‑1 (MQ=255) ggcccGAACGCGCAGCGCGTGCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAg > 2:1062831/3‑75 (MQ=37) ggcccGAACGCGCAGCGCGTGCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAg < 1:1062831/73‑1 (MQ=38) gcccGAACTCGCAGCGCGTGCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCAGatcatc < 1:961123/94‑1 (MQ=255) gcccGAACGCGCAGCGCGTGCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGccc > 2:487197/2‑87 (MQ=38) gcccGAACGCGCAGCGCGTGCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGccc < 1:487197/86‑1 (MQ=38) gcccGAACGCGCAGCGCGTGCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCAGatcatc > 2:961123/2‑95 (MQ=255) cccGCACGCGCTGCGCGTTCGGGACAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCAGATCATCGAGGCGGCGGAAAGGGCCTTCTACAAGCGCGGGGTTGCGCGAACCACCCTGGCCGa < 2:243225/150‑1 (MQ=255) cACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGTCATGGTCCGGCGAACCAAAGAAGAAGCACAGGAAACCCGTGCCCAGATCATCGAGGCGGCTGAAAGGGCCTTCTACAAGCGCGGGGTTGCGTGAACCACCCTGGCCGACATc < 2:480744/150‑1 (MQ=255) cTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCAGATCATCGAGGCGGCGGAAAGGGCCTTCTACAAGCGCGGGGTTGCGCGAACCACCCTGGCCGACATCGCCGaa > 1:823511/1‑150 (MQ=255) tGCGCGTCCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCAGATCATCGAGGCGCCGGAAAGGGCCTTCTACAAGCGCGGGGTTGCGCGAACCACCCTGGCCGACATCGCCGAAc < 2:199757/150‑1 (MQ=255) | AATAGCTTGCTAAGGAATATACTTACATTCATGGTTGTTTGTAAATACTGCTGGGTGGTACTCAGGTACAGCCTCAGCGCTTGATTAGCTTATCCGAGAGGCCCCGGCAGAGGTGCCCCCGGAGCGTTCCCCGCACGCGCTGCGCGTTCGGGCCAGATGAGGTTGTACTGCCATGGTCCGTCGAACCAAAGAAGAAGCCCAGGAAACCCGTGCCCAGATCATCGAGGCGGCGGAAAGGGCCTTCTACAAGCGCGGGGTTGCGCGAACCACCCTGGCCGACATCGCCGAAC > NC_002947/1582682‑1582971 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |