| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,594,292 | G→A | 9.5% | G391G (GGC→GGT) | kgtP ← | alpha‑ketoglutarate permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,594,292 | 0 | G | A | 9.5% | 170.5 / 7.6 | 63 | G391G (GGC→GGT) | kgtP | alpha‑ketoglutarate permease |
| Reads supporting (aligned to +/- strand): ref base G (30/27); new base A (3/3); total (33/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
TCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCA > NC_002947/1594149‑1594439 | tCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGCAccgccg < 1:873510/150‑1 (MQ=255) tGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTGGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGa > 1:666034/1‑150 (MQ=255) gTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATc > 2:242617/1‑150 (MQ=255) gTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATc < 1:882915/150‑1 (MQ=255) tAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCg > 1:4558/1‑150 (MQ=255) aaTCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGa < 2:607968/150‑1 (MQ=255) gAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTc < 1:471609/150‑1 (MQ=255) ttCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAgg > 2:48585/1‑135 (MQ=255) ttCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAgg < 1:48585/135‑1 (MQ=255) tCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAg > 1:1202631/1‑145 (MQ=255) tCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAg < 2:1202631/145‑1 (MQ=255) cATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAAc > 1:740407/1‑150 (MQ=255) tCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCa < 1:484739/150‑1 (MQ=255) gcgTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAggg < 1:1071999/127‑1 (MQ=255) gcgTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAggg > 2:1071999/1‑127 (MQ=255) gcgTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAgg > 2:194896/1‑150 (MQ=255) gTAAACCAGCAGCGAGCAGGCGATACGGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGgc > 1:660763/1‑150 (MQ=255) aaCCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGcgcg > 1:1186144/1‑150 (MQ=255) cagcagCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCTAACGCCCAGGGCGCGAAt > 1:124302/1‑150 (MQ=255) cagcGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTc < 1:242617/150‑1 (MQ=255) agcGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCg < 1:931031/150‑1 (MQ=255) agcGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCg < 2:666034/150‑1 (MQ=255) gcGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGc < 2:373882/126‑1 (MQ=255) gcGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGc > 1:373882/1‑126 (MQ=255) cAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAggg > 1:687573/1‑150 (MQ=255) cAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAggg < 2:1186144/150‑1 (MQ=255) gCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAAc < 1:157144/150‑1 (MQ=255) gCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCACGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAAc > 2:157144/1‑150 (MQ=255) aGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCgg < 2:687573/150‑1 (MQ=255) ggACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGa < 2:660763/149‑1 (MQ=255) tAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGaa > 1:992240/1‑125 (MQ=255) tAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGaa < 2:992240/125‑1 (MQ=255) aaGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATc > 1:785585/1‑150 (MQ=255) gCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGgcgc > 2:344299/1‑104 (MQ=255) gCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGgcgc < 1:344299/104‑1 (MQ=255) cctctttcCATCCCTGCGCTCGTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAg < 2:67026/144‑1 (MQ=255) ccGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTa > 1:1017765/1‑114 (MQ=255) ccGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTa < 2:1017765/114‑1 (MQ=255) ccGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTGGACCACGGCGTTGATCGAg > 2:793829/1‑150 (MQ=255) cGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGt < 1:539158/141‑1 (MQ=255) cGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGt > 2:539158/1‑141 (MQ=255) cGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAgg > 2:870905/1‑150 (MQ=255) aTCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCg > 1:853609/1‑150 (MQ=255) tCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGc > 1:773957/1‑150 (MQ=255) tCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGc < 1:194896/150‑1 (MQ=255) tctTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAAt < 1:52315/88‑1 (MQ=255) tctTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAAt > 2:52315/1‑88 (MQ=255) tctTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCg < 1:1084483/92‑1 (MQ=255) tctTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCg > 2:1084483/1‑92 (MQ=255) cacaGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAg > 2:26215/1‑123 (MQ=255) cacaGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAg < 1:26215/123‑1 (MQ=255) acaGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGNATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATTGAGGTATAGCCGCTTCCGATGCTCAGTGCgg > 1:853394/1‑150 (MQ=255) ggCCACGTATTCGGCGGTGCCGCCGAAGATCGACCCCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCAt < 2:4558/150‑1 (MQ=255) tcgatcttCGGCGGCACCGCCGAATATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGAt > 2:1150383/7‑150 (MQ=255) cACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGAt > 1:230038/1‑150 (MQ=255) aCGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATc > 2:178703/1‑150 (MQ=255) gatcttCGGCGGCACCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATTGAGGTATAg > 1:27983/5‑119 (MQ=255) gatcttCGGCGGCACCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAg < 2:27983/115‑1 (MQ=255) ttCGGCGGCACCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGtt < 1:708571/102‑1 (MQ=255) ttCGGCGGCACCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGtt > 2:708571/1‑102 (MQ=255) ggcggTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCGGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGtt < 1:814971/99‑1 (MQ=255) ggcggTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCGGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGtt > 2:814971/1‑99 (MQ=255) gTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGTCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCa < 2:124302/150‑1 (MQ=255) | TCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCA > NC_002947/1594149‑1594439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |