| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,629,495 | G→T | 8.0% | Q321H (CAG→CAT) | mucB → | sigma factor AlgU regulator MucB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,629,495 | 0 | G | T | 8.0% | 153.6 / 3.0 | 50 | Q321H (CAG→CAT) | mucB | sigma factor AlgU regulator MucB |
| Reads supporting (aligned to +/- strand): ref base G (20/26); new base T (2/2); total (22/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
CTAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAGGGGTCTTT > NC_002947/1629355‑1629643 | cTAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGgcgc < 1:772797/150‑1 (MQ=255) ccGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCtttt > 2:1073011/1‑150 (MQ=255) tCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCATTACTGgcgcgcctgg < 2:1042833/144‑5 (MQ=255) tCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCATTACTGgcgcgcctgg > 1:1042833/1‑140 (MQ=255) tCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGCTGCTGGTTGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGTTGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGc < 1:979472/150‑1 (MQ=255) tctcGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCa > 1:370074/1‑150 (MQ=255) cacCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCACCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGc > 1:498801/1‑150 (MQ=255) cacCCAAGGGCAAAGTGATGGTGACCGTGGTGGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGc < 1:895/150‑1 (MQ=255) aGGGCAAAGTGATGGTTACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCtgaaa < 1:255073/150‑1 (MQ=255) aaaGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCtgaaatgaaa > 1:177928/1‑150 (MQ=255) aaGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCAAGCCAAGGCAGGTGGGCATACGCCTgaaatgaaat > 2:349296/1‑150 (MQ=255) gATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTaaa < 1:399785/150‑1 (MQ=255) gTGACTGTGGTTTTCGAAATTCCGCTTGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCAt < 2:671772/150‑1 (MQ=255) gTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGcc > 2:1088612/1‑106 (MQ=255) gTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGcc < 1:1088612/106‑1 (MQ=255) ggTGGTTGGCGAAATTGCGCTGGGTACGGCAGAACGCGTCGTCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCa < 2:370074/149‑1 (MQ=255) ggTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCGAgg < 1:252231/107‑1 (MQ=255) ggTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCGAgg > 2:252231/1‑107 (MQ=255) ggCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCaa > 2:1023585/1‑150 (MQ=255) ggCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCaa < 1:923491/150‑1 (MQ=255) cGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAg > 1:397272/1‑100 (MQ=255) cGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAg < 2:397272/100‑1 (MQ=255) cGCTGGGTACGGCAGAACGCGTCGCCCAGTGGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCa > 1:822886/1‑150 (MQ=255) gCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCaa > 1:864214/1‑150 (MQ=255) ggCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATc > 1:965331/1‑136 (MQ=255) ggCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATc < 2:965331/136‑1 (MQ=255) cAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGTCGCGACAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAg < 2:822886/150‑1 (MQ=255) cAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTTCCGAGCCAAGGCAGGTGGGCATACGCCTGAAAGGAACTTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAg < 1:930520/150‑1 (MQ=255) aaCGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCTGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTc < 2:498801/150‑1 (MQ=255) tCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCtgaaat > 1:29327/1‑94 (MQ=255) tCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCtgaaat < 2:29327/94‑1 (MQ=255) cccTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGc < 2:976162/84‑1 (MQ=255) cccTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGc > 1:976162/1‑84 (MQ=255) gTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAagag < 1:1023585/150‑1 (MQ=255) tGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGaa < 2:119358/142‑1 (MQ=255) tGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGaa > 1:119358/1‑142 (MQ=255) cccAGCATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCtttt < 1:492645/150‑1 (MQ=255) gATGCCCATGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCg < 1:1073011/150‑1 (MQ=255) cccccAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGtt < 2:1015043/148‑1 (MQ=255) ccAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCNAAGGNAGGNGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTtgag > 1:1015043/1‑148 (MQ=255) ccAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTAGGGATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCAATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTtgtg > 1:821911/1‑150 (MQ=255) cAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGc > 1:123400/1‑150 (MQ=255) aGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCg < 1:1092004/96‑1 (MQ=255) aGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCg > 2:1092004/1‑96 (MQ=255) gcgcgcCATTACTGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGc < 2:336230/140‑1 (MQ=255) gcgcgcCATTACTGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGc > 1:336230/1‑140 (MQ=255) ggggcCAGTTAGGGCGCGCTTTGGCGTGCCGAGCCAATGCAGGTGTGCATACGCCTGAAATGAAATTAGAGCATGGTCATTTGCAAGCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAggg < 2:123400/147‑1 (MQ=255) cAGTAATGGCGCGCTTTTGCGCGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTc < 1:1025707/98‑1 (MQ=255) cAGTAATGGCGCGCTTTTGCGCGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTc > 2:1025707/1‑98 (MQ=255) aGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAGGGGTCttt < 2:864214/150‑1 (MQ=255) | CTAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAGGGGTCTTT > NC_002947/1629355‑1629643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |