| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,632,814 | A→T | 8.6% | Y509F (TAT→TTT) | lepA → | elongation factor 4 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,632,814 | 0 | A | T | 8.6% | 183.5 / 7.1 | 58 | Y509F (TAT→TTT) | lepA | elongation factor 4 |
| Reads supporting (aligned to +/- strand): ref base A (28/25); new base T (2/3); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CCTCAAATCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGC > NC_002947/1632668‑1632962 | ccTCAAATCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATaa > 2:978012/1‑150 (MQ=255) cccaGCCGCGGCTATGCTTCGCTGGATTATCCTTTCTATCGCTACCAGTGGGCGAACCTGGTCAAGCTTGACGTGCTGATCAACGGCGACAAGTTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTTTATAGG‑CCgcgct < 2:250061/149‑2 (MQ=255) accaGCAGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCg > 2:140443/1‑150 (MQ=255) caGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGtt < 2:1109564/150‑1 (MQ=255) gcgGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGtt < 2:118236/145‑1 (MQ=255) gcgGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGtt > 1:118236/1‑145 (MQ=255) tATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGagaag > 2:717905/1‑150 (MQ=255) ttCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGaa > 2:1183330/1‑150 (MQ=255) cTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGaa < 1:13453/150‑1 (MQ=255) tGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAAc > 2:906205/1‑150 (MQ=255) ggATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACt < 1:1183330/150‑1 (MQ=255) ttATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑t < 1:140443/150‑1 (MQ=255) ttATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑t < 2:64346/150‑1 (MQ=255) ttATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑t < 1:906205/150‑1 (MQ=255) tttCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTTTATAGG‑CCgcg > 2:956227/1‑114 (MQ=255) tttCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTTTATAGG‑CCgcg < 1:956227/116‑3 (MQ=255) tCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGAt > 1:17788/1‑150 (MQ=255) tACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAGAGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTc < 1:616388/150‑1 (MQ=255) aCCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAgg > 1:192035/1‑126 (MQ=255) aCCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAgg < 2:192035/126‑1 (MQ=255) aGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGagaaga < 1:30691/117‑1 (MQ=255) aGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGagaaga > 2:30691/1‑117 (MQ=255) gTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑Tcc > 1:714882/1‑131 (MQ=255) gTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑Tcc < 2:714882/131‑1 (MQ=255) gTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGAGGCCTATAAAGG‑CCGTGCGTTGACCgag < 2:575837/112‑1 (MQ=255) gTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGAGGCCTATAAAGG‑CCGTGCGTTGACCgag > 1:575837/1‑112 (MQ=255) ccAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAg > 2:1127880/1‑90 (MQ=255) ccAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAg < 1:1127880/90‑1 (MQ=255) ccAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTc > 1:943239/1‑129 (MQ=255) ccAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTc < 2:943239/129‑1 (MQ=255) cAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGAt > 2:490538/1‑150 (MQ=255) aaCCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGAGCGAG‑AATGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATc > 1:616694/1‑150 (MQ=255) gTCAAGCTGGACGTGCTGATCAACGGCGACCAGGTCGATTCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGT‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCa < 2:230527/150‑1 (MQ=255) tCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAg < 1:717905/150‑1 (MQ=255) gCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCAt > 2:908489/1‑150 (MQ=255) gcGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCGGATGTTCGACGTAGCGATCCAGGc < 1:828232/138‑1 (MQ=255) gcGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGc > 2:828232/2‑139 (MQ=255) gATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGAt < 1:490538/150‑1 (MQ=255) aTCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCAACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATc < 2:354509/150‑1 (MQ=255) aTCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGAACCGCGACAACGCGGCCGATAAAGG‑CCGTGCGTTGCCCGGGAAGTTTAATTAACTGA‑TTCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATc > 1:574617/1‑150 (MQ=255) aTCAACGGCGACAAGGTCGATGCCCTGGCATAGAACGTGCACAGCGACAACGCGGCCGACACAGG‑CCGTTCGATGCCCCAGACGAT‑AACCACGCGAGTGCCTCGGCAGATGTTCGACGTAGGGCTCCAGGCAGCCATTGGCGGCCAGATc > 1:574814/1‑150 (MQ=255) cAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCg > 2:1197586/1‑102 (MQ=255) cAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCg < 1:1197586/102‑1 (MQ=255) aCGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTgg > 2:71014/1‑136 (MQ=255) aCGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTgg < 1:71014/136‑1 (MQ=255) gCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCa < 2:425640/125‑1 (MQ=255) gCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCa > 1:425640/1‑125 (MQ=255) aaGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACa > 2:335398/1‑150 (MQ=255) gTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAAcc > 2:848528/1‑150 (MQ=255) tGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGc < 2:764360/102‑1 (MQ=255) tGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGc > 1:764360/1‑102 (MQ=255) cccTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCGATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAgg < 1:978012/150‑1 (MQ=255) ccTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGgc > 2:714760/1‑150 (MQ=255) tCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCagaaaga > 1:52651/1‑150 (MQ=255) aCCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTAc > 2:498170/1‑150 (MQ=255) gcacgGCCTTTATAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCagaaaga < 2:601230/132‑1 (MQ=255) gcacgGCCTTTATAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCagaaaga > 1:601230/4‑135 (MQ=255) aCGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGt < 1:714760/150‑1 (MQ=255) tataAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTAcggc < 2:574814/150‑1 (MQ=255) tataAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTAcggc < 2:574617/150‑1 (MQ=255) | CCTCAAATCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGG‑CCGTGCGTTGACCGAGAAGATGAAGGAACTGA‑TCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGC > NC_002947/1632668‑1632962 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |