Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,647,612 G→A 100% D384D (GAC→GAT)  oprB‑II ← carbohydrate‑selective porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,647,6120GA100.0% 173.6 / NA 51D384D (GAC→GAT) oprB‑IIcarbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/27);  total (24/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGTCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGCCTGCACATAG  >  NC_002947/1647469‑1647756
                                                                                                                                               |                                                                                                                                                
ccATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATag                                                                                                                                            >  2:466669/1‑150 (MQ=255)
 cATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATaga                                                                                                                                           >  2:674663/1‑150 (MQ=255)
   tCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAACCCGGGTTGTCGTAATCATAGAgg                                                                                                                                         >  2:178451/1‑150 (MQ=255)
      aCCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCcg                                                                                                                                      >  1:788475/1‑150 (MQ=255)
         tGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCcggcg                                                                                                                                   <  1:754009/150‑1 (MQ=255)
               acCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTgg                                                                                                                             <  2:1174049/150‑1 (MQ=255)
                 cccgcccgGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGtt                                                                                                                           >  2:393846/1‑150 (MQ=255)
                 cccgcccgGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGCtt                                                                                                                           >  2:206670/1‑150 (MQ=255)
                     cccgGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGAcc                                                                                                                       <  1:717604/150‑1 (MQ=255)
                      ccgGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCa                                                                                                                      >  2:606785/1‑150 (MQ=255)
                               ggATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGt                                                                                                             >  1:575888/1‑150 (MQ=255)
                                       tGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAACCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGa                                                                                                     <  1:178451/150‑1 (MQ=255)
                                         cAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAggcgg                                                                                                                 <  1:375266/136‑1 (MQ=255)
                                         cAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAggcgg                                                                                                                 >  2:375266/1‑136 (MQ=255)
                                                 ggCGTAGCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGt                                                                                           >  2:46990/1‑150 (MQ=255)
                                                  gCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGtt                                                                                          <  2:426597/150‑1 (MQ=255)
                                                    gTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGa                                                                                        <  2:630739/150‑1 (MQ=255)
                                                    gTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTCAACCAggccgcc                                                                                                               >  2:994789/1‑123 (MQ=255)
                                                    gTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTCAACCAggccgcc                                                                                                               <  1:994789/127‑5 (MQ=255)
                                                              ccAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCAcgcg                                                                              <  1:674663/150‑1 (MQ=255)
                                                               cAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCgg                                                                             <  1:466669/150‑1 (MQ=255)
                                                                aGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCggg                                                                            >  2:1095245/1‑150 (MQ=255)
                                                                      ccAAGTGACTGCCGTAATACCGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTCGATCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAggg                                                                      <  2:470998/150‑1 (MQ=255)
                                                                       cAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCa                                                                                                                      >  1:354177/1‑101 (MQ=255)
                                                                       cAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCa                                                                                                                      <  2:354177/101‑1 (MQ=255)
                                                                       cAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGc                                                                     <  1:1095245/150‑1 (MQ=255)
                                                                         aGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGa                                                                   >  2:567290/1‑150 (MQ=255)
                                                                          gTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGACCGGGTTGTCGTAATCATAGATGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGaa                                                                  >  1:146538/1‑150 (MQ=255)
                                                                              aTGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCg                                                              <  1:1084412/150‑1 (MQ=255)
                                                                               tGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGa                                                             <  1:567290/150‑1 (MQ=255)
                                                                                gCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGAt                                                            <  2:619915/150‑1 (MQ=255)
                                                                                gCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGAt                                                            <  2:575888/150‑1 (MQ=255)
                                                                                  cGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGAc                                                                                                                        >  1:264789/1‑88 (MQ=255)
                                                                                  cGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGAc                                                                                                                        <  2:264789/88‑1 (MQ=255)
                                                                                          aGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTg                                                  <  1:911344/150‑1 (MQ=255)
                                                                                          aGCGCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGAACGCGGGCCAGGGCGAAACCGATGTCGTCCTTg                                                  >  2:203614/1‑150 (MQ=255)
                                                                                            cTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGc                                                                                                       >  1:1073973/1‑95 (MQ=255)
                                                                                            cTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGc                                                                                                       <  2:1073973/95‑1 (MQ=255)
                                                                                                gcgcTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCgg                                            <  1:909752/150‑1 (MQ=255)
                                                                                                 cgcTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCggg                                           <  2:146538/150‑1 (MQ=255)
                                                                                                          cGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTTGCGCGGGCATCGAAAg                                  <  1:393846/150‑1 (MQ=255)
                                                                                                                   gCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTTGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGa                         >  1:1159593/1‑150 (MQ=255)
                                                                                                                       tGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATAc                     <  2:788475/150‑1 (MQ=255)
                                                                                                                        ggCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATAcc                    >  1:522918/1‑150 (MQ=255)
                                                                                                                           aGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGt                 >  2:208549/1‑150 (MQ=255)
                                                                                                                            ggAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTc                >  2:792424/1‑150 (MQ=255)
                                                                                                                                 cccGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTcctgcc           >  1:895752/1‑150 (MQ=255)
                                                                                                                                 cccGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTcctgcc           <  1:46990/150‑1 (MQ=255)
                                                                                                                                  ccGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTcctgcct          <  2:729388/150‑1 (MQ=255)
                                                                                                                                          tCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGCCTGCACATAg  >  1:815632/1‑150 (MQ=255)
                                                                                                                                          tCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGCCTGCACATAg  <  1:208549/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CCATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGTCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGCCTGCACATAG  >  NC_002947/1647469‑1647756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑