| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,718,111 | G→C | 13.4% | L256F (TTG→TTC) | rsmJ → | ribosomal RNA small subunit methyltransferase J |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,718,111 | 0 | G | C | 13.4% | 121.3 / 8.7 | 43 | L256F (TTG→TTC) | rsmJ | ribosomal RNA small subunit methyltransferase J |
| Reads supporting (aligned to +/- strand): ref base G (18/19); new base C (3/3); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCAGTAGTCA > NC_002947/1717962‑1718260 | ccGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTg < 1:1143512/150‑1 (MQ=255) cgcTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAgg < 2:862907/150‑1 (MQ=255) gctgctCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGc > 1:1207948/1‑150 (MQ=255) gctgctCGAAGCCGCTCCGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGc < 1:369722/150‑1 (MQ=255) ctCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGa < 2:424397/150‑1 (MQ=255) ctCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGCCttc < 1:791752/150‑3 (MQ=255) tCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGAc < 1:424386/150‑1 (MQ=255) aGCCGCTCTGGCGCTGGCAAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGc < 2:968983/150‑1 (MQ=255) tAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGa < 1:416347/113‑1 (MQ=255) tAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGa > 2:416347/1‑113 (MQ=255) aaGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCt > 1:477308/1‑150 (MQ=255) aaGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCt > 1:562411/1‑150 (MQ=255) cccACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCt > 2:1158129/1‑101 (MQ=255) cccACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCt < 1:1158129/101‑1 (MQ=255) ccACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGcc > 1:555327/1‑150 (MQ=255) ccACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGcc > 2:643364/1‑150 (MQ=255) ccACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGcc > 1:195007/1‑150 (MQ=255) ggCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAacac < 1:966847/150‑1 (MQ=255) cccGATCAACGACGGGCCGCAGCCGAGCCTTAGCTTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGa < 2:316185/149‑1 (MQ=255) ccGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGAc > 1:885173/1‑150 (MQ=255) cGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGcc > 2:803677/1‑86 (MQ=255) cGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGcc < 1:803677/86‑1 (MQ=255) atcGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCcgc < 1:489349/134‑1 (MQ=255) atcGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCcgc > 2:489349/1‑134 (MQ=255) ccGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATc < 1:643364/150‑1 (MQ=255) aGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGcgtc < 2:477308/150‑1 (MQ=255) aTAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGCCttc > 1:1044649/1‑60 (MQ=255) aTAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGCCttc < 2:1044649/62‑3 (MQ=255) tGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTgcgc < 2:195007/150‑1 (MQ=255) aGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGt < 2:562411/150‑1 (MQ=255) gTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTaa > 2:155019/1‑150 (MQ=255) aCATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAAc > 2:582857/1‑134 (MQ=255) aCATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAAc < 1:582857/134‑1 (MQ=255) cATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGAcc < 1:636512/150‑1 (MQ=255) aTCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAAt < 2:481628/145‑1 (MQ=255) aTCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAAt > 1:481628/1‑145 (MQ=255) tCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTg > 1:915074/1‑150 (MQ=255) ccGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTAtt > 2:1030497/1‑150 (MQ=255) cGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTAttt > 2:199631/1‑150 (MQ=255) gaagaaGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAAc > 1:116447/1‑124 (MQ=255) gaagaaGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAAc < 2:116447/124‑1 (MQ=255) tcagGCCTTCAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGc > 1:307406/3‑150 (MQ=255) gaagGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACaa < 2:836443/133‑1 (MQ=255) gaagGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACaa > 1:836443/1‑133 (MQ=255) gAAGGCCTGACATGACACTATATCGTGGGCTCGAACCATTGTAGCAGCCGCGGTAGGCGCGAACACGGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCAGTAGTCa > 2:919504/1‑150 (MQ=255) | CCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCAGTAGTCA > NC_002947/1717962‑1718260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |